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Epithelial stem cell culture: modeling human disease and applications for regenerative medicine
The inability to maintain the immaturity of stem cell populations in vitro restricts the long-term expansion of various types of human epithelial stem cells. However, recent technical advances in epithelial stem cell culture have led to the development of novel in vitro strategies for regenerating e...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725889/ https://www.ncbi.nlm.nih.gov/pubmed/29259702 http://dx.doi.org/10.1186/s41232-017-0034-9 |
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author | Yamamoto, Yusuke Ochiya, Takahiro |
author_facet | Yamamoto, Yusuke Ochiya, Takahiro |
author_sort | Yamamoto, Yusuke |
collection | PubMed |
description | The inability to maintain the immaturity of stem cell populations in vitro restricts the long-term expansion of various types of human epithelial stem cells. However, recent technical advances in epithelial stem cell culture have led to the development of novel in vitro strategies for regenerating epithelial tissues and for closely mimicking human diseases such as cancer and inflammation. Specifically, improvements in culture conditions provided by small molecules in combination with three-dimensional (3D) culture approaches have facilitated the establishment of in vitro systems that recapitulate biological properties in epithelial organs, and these systems may be used to model disease. In this review article, we describe the biological significance of technical improvements in the development of these methods, focusing on human epithelial cells, including stratified and columnar epithelial cells. We also discuss the potential and future perspectives of this technology, which is only beginning to be explored. |
format | Online Article Text |
id | pubmed-5725889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57258892017-12-19 Epithelial stem cell culture: modeling human disease and applications for regenerative medicine Yamamoto, Yusuke Ochiya, Takahiro Inflamm Regen Review The inability to maintain the immaturity of stem cell populations in vitro restricts the long-term expansion of various types of human epithelial stem cells. However, recent technical advances in epithelial stem cell culture have led to the development of novel in vitro strategies for regenerating epithelial tissues and for closely mimicking human diseases such as cancer and inflammation. Specifically, improvements in culture conditions provided by small molecules in combination with three-dimensional (3D) culture approaches have facilitated the establishment of in vitro systems that recapitulate biological properties in epithelial organs, and these systems may be used to model disease. In this review article, we describe the biological significance of technical improvements in the development of these methods, focusing on human epithelial cells, including stratified and columnar epithelial cells. We also discuss the potential and future perspectives of this technology, which is only beginning to be explored. BioMed Central 2017-02-06 /pmc/articles/PMC5725889/ /pubmed/29259702 http://dx.doi.org/10.1186/s41232-017-0034-9 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Yamamoto, Yusuke Ochiya, Takahiro Epithelial stem cell culture: modeling human disease and applications for regenerative medicine |
title | Epithelial stem cell culture: modeling human disease and applications for regenerative medicine |
title_full | Epithelial stem cell culture: modeling human disease and applications for regenerative medicine |
title_fullStr | Epithelial stem cell culture: modeling human disease and applications for regenerative medicine |
title_full_unstemmed | Epithelial stem cell culture: modeling human disease and applications for regenerative medicine |
title_short | Epithelial stem cell culture: modeling human disease and applications for regenerative medicine |
title_sort | epithelial stem cell culture: modeling human disease and applications for regenerative medicine |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725889/ https://www.ncbi.nlm.nih.gov/pubmed/29259702 http://dx.doi.org/10.1186/s41232-017-0034-9 |
work_keys_str_mv | AT yamamotoyusuke epithelialstemcellculturemodelinghumandiseaseandapplicationsforregenerativemedicine AT ochiyatakahiro epithelialstemcellculturemodelinghumandiseaseandapplicationsforregenerativemedicine |