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Epithelial stem cell culture: modeling human disease and applications for regenerative medicine

The inability to maintain the immaturity of stem cell populations in vitro restricts the long-term expansion of various types of human epithelial stem cells. However, recent technical advances in epithelial stem cell culture have led to the development of novel in vitro strategies for regenerating e...

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Detalles Bibliográficos
Autores principales: Yamamoto, Yusuke, Ochiya, Takahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725889/
https://www.ncbi.nlm.nih.gov/pubmed/29259702
http://dx.doi.org/10.1186/s41232-017-0034-9
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author Yamamoto, Yusuke
Ochiya, Takahiro
author_facet Yamamoto, Yusuke
Ochiya, Takahiro
author_sort Yamamoto, Yusuke
collection PubMed
description The inability to maintain the immaturity of stem cell populations in vitro restricts the long-term expansion of various types of human epithelial stem cells. However, recent technical advances in epithelial stem cell culture have led to the development of novel in vitro strategies for regenerating epithelial tissues and for closely mimicking human diseases such as cancer and inflammation. Specifically, improvements in culture conditions provided by small molecules in combination with three-dimensional (3D) culture approaches have facilitated the establishment of in vitro systems that recapitulate biological properties in epithelial organs, and these systems may be used to model disease. In this review article, we describe the biological significance of technical improvements in the development of these methods, focusing on human epithelial cells, including stratified and columnar epithelial cells. We also discuss the potential and future perspectives of this technology, which is only beginning to be explored.
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spelling pubmed-57258892017-12-19 Epithelial stem cell culture: modeling human disease and applications for regenerative medicine Yamamoto, Yusuke Ochiya, Takahiro Inflamm Regen Review The inability to maintain the immaturity of stem cell populations in vitro restricts the long-term expansion of various types of human epithelial stem cells. However, recent technical advances in epithelial stem cell culture have led to the development of novel in vitro strategies for regenerating epithelial tissues and for closely mimicking human diseases such as cancer and inflammation. Specifically, improvements in culture conditions provided by small molecules in combination with three-dimensional (3D) culture approaches have facilitated the establishment of in vitro systems that recapitulate biological properties in epithelial organs, and these systems may be used to model disease. In this review article, we describe the biological significance of technical improvements in the development of these methods, focusing on human epithelial cells, including stratified and columnar epithelial cells. We also discuss the potential and future perspectives of this technology, which is only beginning to be explored. BioMed Central 2017-02-06 /pmc/articles/PMC5725889/ /pubmed/29259702 http://dx.doi.org/10.1186/s41232-017-0034-9 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Yamamoto, Yusuke
Ochiya, Takahiro
Epithelial stem cell culture: modeling human disease and applications for regenerative medicine
title Epithelial stem cell culture: modeling human disease and applications for regenerative medicine
title_full Epithelial stem cell culture: modeling human disease and applications for regenerative medicine
title_fullStr Epithelial stem cell culture: modeling human disease and applications for regenerative medicine
title_full_unstemmed Epithelial stem cell culture: modeling human disease and applications for regenerative medicine
title_short Epithelial stem cell culture: modeling human disease and applications for regenerative medicine
title_sort epithelial stem cell culture: modeling human disease and applications for regenerative medicine
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725889/
https://www.ncbi.nlm.nih.gov/pubmed/29259702
http://dx.doi.org/10.1186/s41232-017-0034-9
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