A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a ‘self-cleaving’ GFP-expression plasmid

The efficacies of guide RNAs (gRNAs), the short RNA molecules that bind to and determine the sequence specificity of the Streptococcus pyogenes Cas9 nuclease, to mediate DNA cleavage vary dramatically. Thus, the selection of appropriate target sites, and hence spacer sequence, is critical for most a...

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Detalles Bibliográficos
Autores principales: Tálas, András, Kulcsár, Péter István, Weinhardt, Nóra, Borsy, Adrienn, Tóth, Eszter, Szebényi, Kornélia, Krausz, Sarah Laura, Huszár, Krisztina, Vida, István, Sturm, Ádám, Gordos, Bianka, Hoffmann, Orsolya Ivett, Bencsura, Petra, Nyeste, Antal, Ligeti, Zoltán, Fodor, Elfrieda, Welker, Ervin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5726473/
https://www.ncbi.nlm.nih.gov/pubmed/28679166
http://dx.doi.org/10.1093/dnares/dsx029

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