Cargando…

An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity

The process of RNA splicing influences many physiological processes, including plant immunity. However, how plant parasites manipulate host RNA splicing process remains unknown. Here we demonstrate that PsAvr3c, an avirulence effector from oomycete plant pathogen Phytophthora sojae, physically binds...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Jie, Gu, Lianfeng, Zhang, Ying, Yan, Tingxiu, Kong, Guanghui, Kong, Liang, Guo, Baodian, Qiu, Min, Wang, Yang, Jing, Maofeng, Xing, Weiman, Ye, Wenwu, Wu, Zhe, Zhang, Zhengguang, Zheng, Xiaobo, Gijzen, Mark, Wang, Yuanchao, Dong, Suomeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727057/
https://www.ncbi.nlm.nih.gov/pubmed/29233978
http://dx.doi.org/10.1038/s41467-017-02233-5
_version_ 1783285794187771904
author Huang, Jie
Gu, Lianfeng
Zhang, Ying
Yan, Tingxiu
Kong, Guanghui
Kong, Liang
Guo, Baodian
Qiu, Min
Wang, Yang
Jing, Maofeng
Xing, Weiman
Ye, Wenwu
Wu, Zhe
Zhang, Zhengguang
Zheng, Xiaobo
Gijzen, Mark
Wang, Yuanchao
Dong, Suomeng
author_facet Huang, Jie
Gu, Lianfeng
Zhang, Ying
Yan, Tingxiu
Kong, Guanghui
Kong, Liang
Guo, Baodian
Qiu, Min
Wang, Yang
Jing, Maofeng
Xing, Weiman
Ye, Wenwu
Wu, Zhe
Zhang, Zhengguang
Zheng, Xiaobo
Gijzen, Mark
Wang, Yuanchao
Dong, Suomeng
author_sort Huang, Jie
collection PubMed
description The process of RNA splicing influences many physiological processes, including plant immunity. However, how plant parasites manipulate host RNA splicing process remains unknown. Here we demonstrate that PsAvr3c, an avirulence effector from oomycete plant pathogen Phytophthora sojae, physically binds to and stabilizes soybean serine/lysine/arginine-rich proteins GmSKRPs. The SKRPs are novel proteins that associate with a complex that contains plant spliceosome components, and are negative regulators of plant immunity. Analysis by RNA-seq data indicates that alternative splicing of pre-mRNAs from 401 soybean genes, including defense-related genes, is altered in GmSKRP1 and PsAvr3c overexpressing lines compared to control plants. Representative splicing events mediated by GmSKRP1 and PsAvr3c are tested by infection assays or by transient expression in soybean plants. Our results show that plant pathogen effectors can reprogram host pre-mRNA splicing to promote disease, and we propose that pathogens evolved such strategies to defeat host immune systems.
format Online
Article
Text
id pubmed-5727057
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-57270572017-12-14 An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity Huang, Jie Gu, Lianfeng Zhang, Ying Yan, Tingxiu Kong, Guanghui Kong, Liang Guo, Baodian Qiu, Min Wang, Yang Jing, Maofeng Xing, Weiman Ye, Wenwu Wu, Zhe Zhang, Zhengguang Zheng, Xiaobo Gijzen, Mark Wang, Yuanchao Dong, Suomeng Nat Commun Article The process of RNA splicing influences many physiological processes, including plant immunity. However, how plant parasites manipulate host RNA splicing process remains unknown. Here we demonstrate that PsAvr3c, an avirulence effector from oomycete plant pathogen Phytophthora sojae, physically binds to and stabilizes soybean serine/lysine/arginine-rich proteins GmSKRPs. The SKRPs are novel proteins that associate with a complex that contains plant spliceosome components, and are negative regulators of plant immunity. Analysis by RNA-seq data indicates that alternative splicing of pre-mRNAs from 401 soybean genes, including defense-related genes, is altered in GmSKRP1 and PsAvr3c overexpressing lines compared to control plants. Representative splicing events mediated by GmSKRP1 and PsAvr3c are tested by infection assays or by transient expression in soybean plants. Our results show that plant pathogen effectors can reprogram host pre-mRNA splicing to promote disease, and we propose that pathogens evolved such strategies to defeat host immune systems. Nature Publishing Group UK 2017-12-12 /pmc/articles/PMC5727057/ /pubmed/29233978 http://dx.doi.org/10.1038/s41467-017-02233-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commonslicense, unless indicated otherwise in a credit line to the material. If material is not included in the article’sCreative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Huang, Jie
Gu, Lianfeng
Zhang, Ying
Yan, Tingxiu
Kong, Guanghui
Kong, Liang
Guo, Baodian
Qiu, Min
Wang, Yang
Jing, Maofeng
Xing, Weiman
Ye, Wenwu
Wu, Zhe
Zhang, Zhengguang
Zheng, Xiaobo
Gijzen, Mark
Wang, Yuanchao
Dong, Suomeng
An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity
title An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity
title_full An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity
title_fullStr An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity
title_full_unstemmed An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity
title_short An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity
title_sort oomycete plant pathogen reprograms host pre-mrna splicing to subvert immunity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727057/
https://www.ncbi.nlm.nih.gov/pubmed/29233978
http://dx.doi.org/10.1038/s41467-017-02233-5
work_keys_str_mv AT huangjie anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT gulianfeng anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT zhangying anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT yantingxiu anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT kongguanghui anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT kongliang anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT guobaodian anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT qiumin anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT wangyang anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT jingmaofeng anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT xingweiman anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT yewenwu anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT wuzhe anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT zhangzhengguang anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT zhengxiaobo anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT gijzenmark anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT wangyuanchao anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT dongsuomeng anoomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT huangjie oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT gulianfeng oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT zhangying oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT yantingxiu oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT kongguanghui oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT kongliang oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT guobaodian oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT qiumin oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT wangyang oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT jingmaofeng oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT xingweiman oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT yewenwu oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT wuzhe oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT zhangzhengguang oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT zhengxiaobo oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT gijzenmark oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT wangyuanchao oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity
AT dongsuomeng oomyceteplantpathogenreprogramshostpremrnasplicingtosubvertimmunity