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An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity
The process of RNA splicing influences many physiological processes, including plant immunity. However, how plant parasites manipulate host RNA splicing process remains unknown. Here we demonstrate that PsAvr3c, an avirulence effector from oomycete plant pathogen Phytophthora sojae, physically binds...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727057/ https://www.ncbi.nlm.nih.gov/pubmed/29233978 http://dx.doi.org/10.1038/s41467-017-02233-5 |
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author | Huang, Jie Gu, Lianfeng Zhang, Ying Yan, Tingxiu Kong, Guanghui Kong, Liang Guo, Baodian Qiu, Min Wang, Yang Jing, Maofeng Xing, Weiman Ye, Wenwu Wu, Zhe Zhang, Zhengguang Zheng, Xiaobo Gijzen, Mark Wang, Yuanchao Dong, Suomeng |
author_facet | Huang, Jie Gu, Lianfeng Zhang, Ying Yan, Tingxiu Kong, Guanghui Kong, Liang Guo, Baodian Qiu, Min Wang, Yang Jing, Maofeng Xing, Weiman Ye, Wenwu Wu, Zhe Zhang, Zhengguang Zheng, Xiaobo Gijzen, Mark Wang, Yuanchao Dong, Suomeng |
author_sort | Huang, Jie |
collection | PubMed |
description | The process of RNA splicing influences many physiological processes, including plant immunity. However, how plant parasites manipulate host RNA splicing process remains unknown. Here we demonstrate that PsAvr3c, an avirulence effector from oomycete plant pathogen Phytophthora sojae, physically binds to and stabilizes soybean serine/lysine/arginine-rich proteins GmSKRPs. The SKRPs are novel proteins that associate with a complex that contains plant spliceosome components, and are negative regulators of plant immunity. Analysis by RNA-seq data indicates that alternative splicing of pre-mRNAs from 401 soybean genes, including defense-related genes, is altered in GmSKRP1 and PsAvr3c overexpressing lines compared to control plants. Representative splicing events mediated by GmSKRP1 and PsAvr3c are tested by infection assays or by transient expression in soybean plants. Our results show that plant pathogen effectors can reprogram host pre-mRNA splicing to promote disease, and we propose that pathogens evolved such strategies to defeat host immune systems. |
format | Online Article Text |
id | pubmed-5727057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57270572017-12-14 An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity Huang, Jie Gu, Lianfeng Zhang, Ying Yan, Tingxiu Kong, Guanghui Kong, Liang Guo, Baodian Qiu, Min Wang, Yang Jing, Maofeng Xing, Weiman Ye, Wenwu Wu, Zhe Zhang, Zhengguang Zheng, Xiaobo Gijzen, Mark Wang, Yuanchao Dong, Suomeng Nat Commun Article The process of RNA splicing influences many physiological processes, including plant immunity. However, how plant parasites manipulate host RNA splicing process remains unknown. Here we demonstrate that PsAvr3c, an avirulence effector from oomycete plant pathogen Phytophthora sojae, physically binds to and stabilizes soybean serine/lysine/arginine-rich proteins GmSKRPs. The SKRPs are novel proteins that associate with a complex that contains plant spliceosome components, and are negative regulators of plant immunity. Analysis by RNA-seq data indicates that alternative splicing of pre-mRNAs from 401 soybean genes, including defense-related genes, is altered in GmSKRP1 and PsAvr3c overexpressing lines compared to control plants. Representative splicing events mediated by GmSKRP1 and PsAvr3c are tested by infection assays or by transient expression in soybean plants. Our results show that plant pathogen effectors can reprogram host pre-mRNA splicing to promote disease, and we propose that pathogens evolved such strategies to defeat host immune systems. Nature Publishing Group UK 2017-12-12 /pmc/articles/PMC5727057/ /pubmed/29233978 http://dx.doi.org/10.1038/s41467-017-02233-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commonslicense, unless indicated otherwise in a credit line to the material. If material is not included in the article’sCreative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Huang, Jie Gu, Lianfeng Zhang, Ying Yan, Tingxiu Kong, Guanghui Kong, Liang Guo, Baodian Qiu, Min Wang, Yang Jing, Maofeng Xing, Weiman Ye, Wenwu Wu, Zhe Zhang, Zhengguang Zheng, Xiaobo Gijzen, Mark Wang, Yuanchao Dong, Suomeng An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity |
title | An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity |
title_full | An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity |
title_fullStr | An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity |
title_full_unstemmed | An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity |
title_short | An oomycete plant pathogen reprograms host pre-mRNA splicing to subvert immunity |
title_sort | oomycete plant pathogen reprograms host pre-mrna splicing to subvert immunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727057/ https://www.ncbi.nlm.nih.gov/pubmed/29233978 http://dx.doi.org/10.1038/s41467-017-02233-5 |
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