Cargando…

Factors affecting the bacterial community composition and heterotrophic production of Columbia River estuarine turbidity maxima

Estuarine turbidity maxima (ETM) function as hotspots of microbial activity and diversity in estuaries, yet, little is known about the temporal and spatial variability in ETM bacterial community composition. To determine which environmental factors affect ETM bacterial populations in the Columbia Ri...

Descripción completa

Detalles Bibliográficos
Autores principales: Herfort, Lydie, Crump, Byron C., Fortunato, Caroline S., McCue, Lee Ann, Campbell, Victoria, Simon, Holly M., Baptista, António M., Zuber, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727365/
https://www.ncbi.nlm.nih.gov/pubmed/28782284
http://dx.doi.org/10.1002/mbo3.522
_version_ 1783285864554561536
author Herfort, Lydie
Crump, Byron C.
Fortunato, Caroline S.
McCue, Lee Ann
Campbell, Victoria
Simon, Holly M.
Baptista, António M.
Zuber, Peter
author_facet Herfort, Lydie
Crump, Byron C.
Fortunato, Caroline S.
McCue, Lee Ann
Campbell, Victoria
Simon, Holly M.
Baptista, António M.
Zuber, Peter
author_sort Herfort, Lydie
collection PubMed
description Estuarine turbidity maxima (ETM) function as hotspots of microbial activity and diversity in estuaries, yet, little is known about the temporal and spatial variability in ETM bacterial community composition. To determine which environmental factors affect ETM bacterial populations in the Columbia River estuary, we analyzed ETM bacterial community composition (Sanger sequencing and amplicon pyrosequencing of 16S rRNA gene) and bulk heterotrophic production ((3)H‐leucine incorporation rates). We collected water 20 times to cover five ETM events and obtained 42 samples characterized by different salinities, turbidities, seasons, coastal regimes (upwelling vs. downwelling), locations, and particle size. Spring and summer populations were distinct. All May samples had similar bacterial community composition despite having different salinities (1–24 PSU), but summer non‐ETM bacteria separated into marine, freshwater, and brackish assemblages. Summer ETM bacterial communities varied depending on coastal upwelling or downwelling conditions and on the sampling site location with respect to tidal intrusion during the previous neap tide. In contrast to ETM, whole (>0.2 μm) and free‐living (0.2–3 μm) assemblages of non‐ETM waters were similar to each other, indicating that particle‐attached (>3 μm) non‐ETM bacteria do not develop a distinct community. Brackish water type (ETM or non‐ETM) is thus a major factor affecting particle‐attached bacterial communities. Heterotrophic production was higher in particle‐attached than free‐living fractions in all brackish waters collected throughout the water column during the rise to decline of turbidity through an ETM event (i.e., ETM‐impacted waters). However, free‐living communities showed higher productivity prior to or after an ETM event (i.e., non‐ETM‐impacted waters). This study has thus found that Columbia River ETM bacterial communities vary based on seasons, salinity, sampling location, and particle size, with the existence of three particle types characterized by different bacterial communities in ETM, ETM‐impacted, and non‐ETM‐impacted brackish waters. Taxonomic analysis suggests that ETM key biological function is to remineralize organic matter.
format Online
Article
Text
id pubmed-5727365
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-57273652017-12-18 Factors affecting the bacterial community composition and heterotrophic production of Columbia River estuarine turbidity maxima Herfort, Lydie Crump, Byron C. Fortunato, Caroline S. McCue, Lee Ann Campbell, Victoria Simon, Holly M. Baptista, António M. Zuber, Peter Microbiologyopen Original Research Estuarine turbidity maxima (ETM) function as hotspots of microbial activity and diversity in estuaries, yet, little is known about the temporal and spatial variability in ETM bacterial community composition. To determine which environmental factors affect ETM bacterial populations in the Columbia River estuary, we analyzed ETM bacterial community composition (Sanger sequencing and amplicon pyrosequencing of 16S rRNA gene) and bulk heterotrophic production ((3)H‐leucine incorporation rates). We collected water 20 times to cover five ETM events and obtained 42 samples characterized by different salinities, turbidities, seasons, coastal regimes (upwelling vs. downwelling), locations, and particle size. Spring and summer populations were distinct. All May samples had similar bacterial community composition despite having different salinities (1–24 PSU), but summer non‐ETM bacteria separated into marine, freshwater, and brackish assemblages. Summer ETM bacterial communities varied depending on coastal upwelling or downwelling conditions and on the sampling site location with respect to tidal intrusion during the previous neap tide. In contrast to ETM, whole (>0.2 μm) and free‐living (0.2–3 μm) assemblages of non‐ETM waters were similar to each other, indicating that particle‐attached (>3 μm) non‐ETM bacteria do not develop a distinct community. Brackish water type (ETM or non‐ETM) is thus a major factor affecting particle‐attached bacterial communities. Heterotrophic production was higher in particle‐attached than free‐living fractions in all brackish waters collected throughout the water column during the rise to decline of turbidity through an ETM event (i.e., ETM‐impacted waters). However, free‐living communities showed higher productivity prior to or after an ETM event (i.e., non‐ETM‐impacted waters). This study has thus found that Columbia River ETM bacterial communities vary based on seasons, salinity, sampling location, and particle size, with the existence of three particle types characterized by different bacterial communities in ETM, ETM‐impacted, and non‐ETM‐impacted brackish waters. Taxonomic analysis suggests that ETM key biological function is to remineralize organic matter. John Wiley and Sons Inc. 2017-08-06 /pmc/articles/PMC5727365/ /pubmed/28782284 http://dx.doi.org/10.1002/mbo3.522 Text en © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Herfort, Lydie
Crump, Byron C.
Fortunato, Caroline S.
McCue, Lee Ann
Campbell, Victoria
Simon, Holly M.
Baptista, António M.
Zuber, Peter
Factors affecting the bacterial community composition and heterotrophic production of Columbia River estuarine turbidity maxima
title Factors affecting the bacterial community composition and heterotrophic production of Columbia River estuarine turbidity maxima
title_full Factors affecting the bacterial community composition and heterotrophic production of Columbia River estuarine turbidity maxima
title_fullStr Factors affecting the bacterial community composition and heterotrophic production of Columbia River estuarine turbidity maxima
title_full_unstemmed Factors affecting the bacterial community composition and heterotrophic production of Columbia River estuarine turbidity maxima
title_short Factors affecting the bacterial community composition and heterotrophic production of Columbia River estuarine turbidity maxima
title_sort factors affecting the bacterial community composition and heterotrophic production of columbia river estuarine turbidity maxima
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727365/
https://www.ncbi.nlm.nih.gov/pubmed/28782284
http://dx.doi.org/10.1002/mbo3.522
work_keys_str_mv AT herfortlydie factorsaffectingthebacterialcommunitycompositionandheterotrophicproductionofcolumbiariverestuarineturbiditymaxima
AT crumpbyronc factorsaffectingthebacterialcommunitycompositionandheterotrophicproductionofcolumbiariverestuarineturbiditymaxima
AT fortunatocarolines factorsaffectingthebacterialcommunitycompositionandheterotrophicproductionofcolumbiariverestuarineturbiditymaxima
AT mccueleeann factorsaffectingthebacterialcommunitycompositionandheterotrophicproductionofcolumbiariverestuarineturbiditymaxima
AT campbellvictoria factorsaffectingthebacterialcommunitycompositionandheterotrophicproductionofcolumbiariverestuarineturbiditymaxima
AT simonhollym factorsaffectingthebacterialcommunitycompositionandheterotrophicproductionofcolumbiariverestuarineturbiditymaxima
AT baptistaantoniom factorsaffectingthebacterialcommunitycompositionandheterotrophicproductionofcolumbiariverestuarineturbiditymaxima
AT zuberpeter factorsaffectingthebacterialcommunitycompositionandheterotrophicproductionofcolumbiariverestuarineturbiditymaxima