Structural insight into catalytic mechanism of PET hydrolase

PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and...

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Detalles Bibliográficos
Autores principales: Han, Xu, Liu, Weidong, Huang, Jian-Wen, Ma, Jiantao, Zheng, Yingying, Ko, Tzu-Ping, Xu, Limin, Cheng, Ya-Shan, Chen, Chun-Chi, Guo, Rey-Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727383/
https://www.ncbi.nlm.nih.gov/pubmed/29235460
http://dx.doi.org/10.1038/s41467-017-02255-z
Descripción
Sumario:PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.

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