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Sexual recombination and increased mutation rate expedite evolution of Escherichia coli in varied fitness landscapes
Sexual recombination and mutation rate are theorized to play different roles in adaptive evolution depending on the fitness landscape; however, direct experimental support is limited. Here we examine how these factors affect the rate of adaptation utilizing a “genderless” strain of Escherichia coli...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727395/ https://www.ncbi.nlm.nih.gov/pubmed/29235478 http://dx.doi.org/10.1038/s41467-017-02323-4 |
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author | Peabody V, George L. Li, Hao Kao, Katy C. |
author_facet | Peabody V, George L. Li, Hao Kao, Katy C. |
author_sort | Peabody V, George L. |
collection | PubMed |
description | Sexual recombination and mutation rate are theorized to play different roles in adaptive evolution depending on the fitness landscape; however, direct experimental support is limited. Here we examine how these factors affect the rate of adaptation utilizing a “genderless” strain of Escherichia coli capable of continuous in situ sexual recombination. The results show that the populations with increased mutation rate, and capable of sexual recombination, outperform all the other populations. We further characterize two sexual and two asexual populations with increased mutation rate and observe maintenance of beneficial mutations in the sexual populations through mutational sweeps. Furthermore, we experimentally identify the molecular signature of a mating event within the sexual population that combines two beneficial mutations to generate a fitter progeny; this evidence suggests that the recombination event partially alleviates clonal interference. We present additional data suggesting that stochasticity plays an important role in the combinations of mutations observed. |
format | Online Article Text |
id | pubmed-5727395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57273952017-12-14 Sexual recombination and increased mutation rate expedite evolution of Escherichia coli in varied fitness landscapes Peabody V, George L. Li, Hao Kao, Katy C. Nat Commun Article Sexual recombination and mutation rate are theorized to play different roles in adaptive evolution depending on the fitness landscape; however, direct experimental support is limited. Here we examine how these factors affect the rate of adaptation utilizing a “genderless” strain of Escherichia coli capable of continuous in situ sexual recombination. The results show that the populations with increased mutation rate, and capable of sexual recombination, outperform all the other populations. We further characterize two sexual and two asexual populations with increased mutation rate and observe maintenance of beneficial mutations in the sexual populations through mutational sweeps. Furthermore, we experimentally identify the molecular signature of a mating event within the sexual population that combines two beneficial mutations to generate a fitter progeny; this evidence suggests that the recombination event partially alleviates clonal interference. We present additional data suggesting that stochasticity plays an important role in the combinations of mutations observed. Nature Publishing Group UK 2017-12-13 /pmc/articles/PMC5727395/ /pubmed/29235478 http://dx.doi.org/10.1038/s41467-017-02323-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Peabody V, George L. Li, Hao Kao, Katy C. Sexual recombination and increased mutation rate expedite evolution of Escherichia coli in varied fitness landscapes |
title | Sexual recombination and increased mutation rate expedite evolution of Escherichia coli in varied fitness landscapes |
title_full | Sexual recombination and increased mutation rate expedite evolution of Escherichia coli in varied fitness landscapes |
title_fullStr | Sexual recombination and increased mutation rate expedite evolution of Escherichia coli in varied fitness landscapes |
title_full_unstemmed | Sexual recombination and increased mutation rate expedite evolution of Escherichia coli in varied fitness landscapes |
title_short | Sexual recombination and increased mutation rate expedite evolution of Escherichia coli in varied fitness landscapes |
title_sort | sexual recombination and increased mutation rate expedite evolution of escherichia coli in varied fitness landscapes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727395/ https://www.ncbi.nlm.nih.gov/pubmed/29235478 http://dx.doi.org/10.1038/s41467-017-02323-4 |
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