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Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study

[Image: see text] The purpose of this study was to evaluate the effects of surface properties of bone implants coated with hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) on platelets and macrophages upon implant installation and compare them to grit-blasted Ti and Thermanox used as a control...

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Autores principales: Fernandes, Kelly R., Zhang, Yang, Magri, Angela M. P., Renno, Ana C. M., van den Beucken, Jeroen J. J. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727470/
https://www.ncbi.nlm.nih.gov/pubmed/29250594
http://dx.doi.org/10.1021/acsbiomaterials.7b00679
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author Fernandes, Kelly R.
Zhang, Yang
Magri, Angela M. P.
Renno, Ana C. M.
van den Beucken, Jeroen J. J. P.
author_facet Fernandes, Kelly R.
Zhang, Yang
Magri, Angela M. P.
Renno, Ana C. M.
van den Beucken, Jeroen J. J. P.
author_sort Fernandes, Kelly R.
collection PubMed
description [Image: see text] The purpose of this study was to evaluate the effects of surface properties of bone implants coated with hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) on platelets and macrophages upon implant installation and compare them to grit-blasted Ti and Thermanox used as a control. Surface properties were characterized using scanning electron microscopy, profilometry, crystallography, Fourier transform infrared spectroscopy, and coating stability. For platelets, platelet adherence and morphology were assessed. For macrophages, morphology, proliferation, and polarization were evaluated. Surface characterization showed similar roughness of ∼2.5 μm for grit-blasted Ti discs, both with and without coating. Coating stability assessment showed substantial dissolution of HA and β-TCP coatings. Platelet adherence was significantly higher for grit-blasted Ti, Ti-HA, and Ti-β-TCP coatings compared to that of cell culture control Thermanox. Macrophage cultures revealed a decreased proliferation on both HA and β-TCP coated discs compared to both Thermanox and grit-blasted Ti. In contrast, secretion of pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine TGF-β were marginal for grit-blasted Ti and Thermanox, while a coating-dependent increased secretion of pro- and anti-inflammatory cytokines was observed for HA and β-TCP coatings. The results demonstrated a significantly upregulated pro-inflammatory and anti-inflammatory cytokine secretion and marker gene expression of macrophages on HA and β-TCP coatings. Furthermore, HA induced an earlier M1 macrophage polarization but more M2 phenotype potency than β-TCP. In conclusion, our data showed that material surface affects the behaviors of first cell types attached to implants. Due to the demonstrated crucial roles of platelets and macrophages in bone healing and implant integration, this information will greatly aid the design of metallic implants for a higher rate of success in patients.
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spelling pubmed-57274702017-12-14 Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study Fernandes, Kelly R. Zhang, Yang Magri, Angela M. P. Renno, Ana C. M. van den Beucken, Jeroen J. J. P. ACS Biomater Sci Eng [Image: see text] The purpose of this study was to evaluate the effects of surface properties of bone implants coated with hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) on platelets and macrophages upon implant installation and compare them to grit-blasted Ti and Thermanox used as a control. Surface properties were characterized using scanning electron microscopy, profilometry, crystallography, Fourier transform infrared spectroscopy, and coating stability. For platelets, platelet adherence and morphology were assessed. For macrophages, morphology, proliferation, and polarization were evaluated. Surface characterization showed similar roughness of ∼2.5 μm for grit-blasted Ti discs, both with and without coating. Coating stability assessment showed substantial dissolution of HA and β-TCP coatings. Platelet adherence was significantly higher for grit-blasted Ti, Ti-HA, and Ti-β-TCP coatings compared to that of cell culture control Thermanox. Macrophage cultures revealed a decreased proliferation on both HA and β-TCP coated discs compared to both Thermanox and grit-blasted Ti. In contrast, secretion of pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine TGF-β were marginal for grit-blasted Ti and Thermanox, while a coating-dependent increased secretion of pro- and anti-inflammatory cytokines was observed for HA and β-TCP coatings. The results demonstrated a significantly upregulated pro-inflammatory and anti-inflammatory cytokine secretion and marker gene expression of macrophages on HA and β-TCP coatings. Furthermore, HA induced an earlier M1 macrophage polarization but more M2 phenotype potency than β-TCP. In conclusion, our data showed that material surface affects the behaviors of first cell types attached to implants. Due to the demonstrated crucial roles of platelets and macrophages in bone healing and implant integration, this information will greatly aid the design of metallic implants for a higher rate of success in patients. American Chemical Society 2017-11-07 2017-12-11 /pmc/articles/PMC5727470/ /pubmed/29250594 http://dx.doi.org/10.1021/acsbiomaterials.7b00679 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Fernandes, Kelly R.
Zhang, Yang
Magri, Angela M. P.
Renno, Ana C. M.
van den Beucken, Jeroen J. J. P.
Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study
title Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study
title_full Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study
title_fullStr Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study
title_full_unstemmed Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study
title_short Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study
title_sort biomaterial property effects on platelets and macrophages: an in vitro study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727470/
https://www.ncbi.nlm.nih.gov/pubmed/29250594
http://dx.doi.org/10.1021/acsbiomaterials.7b00679
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