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One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles
MS2 phage-like particles (MS2 PLP) are artificially constructed pseudo-viral particles derived from bacteriophage MS2. They are able to carry a specific single stranded RNA (ssRNA) sequence of choice inside their capsid, thus protecting it against the effects of ubiquitous nucleases. Such particles...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727534/ https://www.ncbi.nlm.nih.gov/pubmed/29235545 http://dx.doi.org/10.1038/s41598-017-17951-5 |
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author | Mikel, Pavel Vasickova, Petra Kralik, Petr |
author_facet | Mikel, Pavel Vasickova, Petra Kralik, Petr |
author_sort | Mikel, Pavel |
collection | PubMed |
description | MS2 phage-like particles (MS2 PLP) are artificially constructed pseudo-viral particles derived from bacteriophage MS2. They are able to carry a specific single stranded RNA (ssRNA) sequence of choice inside their capsid, thus protecting it against the effects of ubiquitous nucleases. Such particles are able to mimic ssRNA viruses and, thus, may serve as the process control for molecular detection and quantification of such agents in several kinds of matrices, vaccines and vaccine candidates, drug delivery systems, and systems for the display of immunologically active peptides or nanomachines. Currently, there are several different in vivo plasmid-driven packaging systems for production of MS2 PLP. In order to combine all the advantages of the available systems and to upgrade and simplify the production and purification of MS2 PLP, a one-plasmid double-expression His-tag system was designed. The described system utilizes a unique fusion insertional mutation enabling purification of particles using His-tag affinity. Using this new production system, highly pure MS2 PLP can be quickly produced and purified by a fast performance liquid chromatography (FPLC) approach. The system can be easily adapted to produce other MS2 PLP with different properties. |
format | Online Article Text |
id | pubmed-5727534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57275342017-12-18 One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles Mikel, Pavel Vasickova, Petra Kralik, Petr Sci Rep Article MS2 phage-like particles (MS2 PLP) are artificially constructed pseudo-viral particles derived from bacteriophage MS2. They are able to carry a specific single stranded RNA (ssRNA) sequence of choice inside their capsid, thus protecting it against the effects of ubiquitous nucleases. Such particles are able to mimic ssRNA viruses and, thus, may serve as the process control for molecular detection and quantification of such agents in several kinds of matrices, vaccines and vaccine candidates, drug delivery systems, and systems for the display of immunologically active peptides or nanomachines. Currently, there are several different in vivo plasmid-driven packaging systems for production of MS2 PLP. In order to combine all the advantages of the available systems and to upgrade and simplify the production and purification of MS2 PLP, a one-plasmid double-expression His-tag system was designed. The described system utilizes a unique fusion insertional mutation enabling purification of particles using His-tag affinity. Using this new production system, highly pure MS2 PLP can be quickly produced and purified by a fast performance liquid chromatography (FPLC) approach. The system can be easily adapted to produce other MS2 PLP with different properties. Nature Publishing Group UK 2017-12-13 /pmc/articles/PMC5727534/ /pubmed/29235545 http://dx.doi.org/10.1038/s41598-017-17951-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Mikel, Pavel Vasickova, Petra Kralik, Petr One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles |
title | One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles |
title_full | One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles |
title_fullStr | One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles |
title_full_unstemmed | One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles |
title_short | One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles |
title_sort | one-plasmid double-expression his-tag system for rapid production and easy purification of ms2 phage-like particles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727534/ https://www.ncbi.nlm.nih.gov/pubmed/29235545 http://dx.doi.org/10.1038/s41598-017-17951-5 |
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