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Computational identification of microRNAs and their targets in liver cirrhosis

Previous studies have revealed that the deregulation of circulating miRNAs is associated with liver cirrhosis. The present study aimed to identify reliable candidate biomarkers to improve the early detection of liver cirrhosis. An integrated analysis of expression profiles of microRNAs (miRNAs/miRs)...

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Autores principales: Du, Hongbo, Yu, Hao, Yang, Yuying, Song, Yuanyuan, Wang, Fei, Li, Shangheng, Jiang, Yuyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727606/
https://www.ncbi.nlm.nih.gov/pubmed/29250171
http://dx.doi.org/10.3892/ol.2017.7252
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author Du, Hongbo
Yu, Hao
Yang, Yuying
Song, Yuanyuan
Wang, Fei
Li, Shangheng
Jiang, Yuyong
author_facet Du, Hongbo
Yu, Hao
Yang, Yuying
Song, Yuanyuan
Wang, Fei
Li, Shangheng
Jiang, Yuyong
author_sort Du, Hongbo
collection PubMed
description Previous studies have revealed that the deregulation of circulating miRNAs is associated with liver cirrhosis. The present study aimed to identify reliable candidate biomarkers to improve the early detection of liver cirrhosis. An integrated analysis of expression profiles of microRNAs (miRNAs/miRs) and mRNAs in liver cirrhosis tissues from the GEO database was performed. Next, the regulatory targets of the differentially expressed miRNAs in liver cirrhosis tissues were predicted. In addition, a regulatory network of miRNA-target genes was constructed. A total of 4 eligible mRNA expression profiling studies and 2 miRNA expression profiling studies met the inclusion criteria, and were thus included. A total of 48 differentially expressed miRNAs and 1,773 differentially expressed genes were identified in liver cirrhosis tissues compared with normal tissues. There were 240 miRNA-target pairs whose expression was negatively correlated. In the miRNA-target regulatory network, overexpression of miR-21 and miR-199a-3p was suggested to be closely associated with the progression of liver cirrhosis. In addition, functional enrichment analysis of the target genes indicated that cell cycle was the most significantly enriched pathway, and the dysregulation of leukemia inhibitory factor, cancerous inhibitor of protein phosphatase 2A and retinoblastoma-associated protein 1 clearly suggested their importance in the development of liver cirrhosis. We hypothesized that miR-21 and miR-199a-3p may be promising non-invasive diagnostic biomarkers for the early diagnosis of liver cirrhosis. The miRNA-target regulatory network may provide additional insight into the current data regarding the role of miRNAs in liver cirrhosis.
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spelling pubmed-57276062017-12-17 Computational identification of microRNAs and their targets in liver cirrhosis Du, Hongbo Yu, Hao Yang, Yuying Song, Yuanyuan Wang, Fei Li, Shangheng Jiang, Yuyong Oncol Lett Articles Previous studies have revealed that the deregulation of circulating miRNAs is associated with liver cirrhosis. The present study aimed to identify reliable candidate biomarkers to improve the early detection of liver cirrhosis. An integrated analysis of expression profiles of microRNAs (miRNAs/miRs) and mRNAs in liver cirrhosis tissues from the GEO database was performed. Next, the regulatory targets of the differentially expressed miRNAs in liver cirrhosis tissues were predicted. In addition, a regulatory network of miRNA-target genes was constructed. A total of 4 eligible mRNA expression profiling studies and 2 miRNA expression profiling studies met the inclusion criteria, and were thus included. A total of 48 differentially expressed miRNAs and 1,773 differentially expressed genes were identified in liver cirrhosis tissues compared with normal tissues. There were 240 miRNA-target pairs whose expression was negatively correlated. In the miRNA-target regulatory network, overexpression of miR-21 and miR-199a-3p was suggested to be closely associated with the progression of liver cirrhosis. In addition, functional enrichment analysis of the target genes indicated that cell cycle was the most significantly enriched pathway, and the dysregulation of leukemia inhibitory factor, cancerous inhibitor of protein phosphatase 2A and retinoblastoma-associated protein 1 clearly suggested their importance in the development of liver cirrhosis. We hypothesized that miR-21 and miR-199a-3p may be promising non-invasive diagnostic biomarkers for the early diagnosis of liver cirrhosis. The miRNA-target regulatory network may provide additional insight into the current data regarding the role of miRNAs in liver cirrhosis. D.A. Spandidos 2017-12 2017-10-23 /pmc/articles/PMC5727606/ /pubmed/29250171 http://dx.doi.org/10.3892/ol.2017.7252 Text en Copyright: © Du et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Du, Hongbo
Yu, Hao
Yang, Yuying
Song, Yuanyuan
Wang, Fei
Li, Shangheng
Jiang, Yuyong
Computational identification of microRNAs and their targets in liver cirrhosis
title Computational identification of microRNAs and their targets in liver cirrhosis
title_full Computational identification of microRNAs and their targets in liver cirrhosis
title_fullStr Computational identification of microRNAs and their targets in liver cirrhosis
title_full_unstemmed Computational identification of microRNAs and their targets in liver cirrhosis
title_short Computational identification of microRNAs and their targets in liver cirrhosis
title_sort computational identification of micrornas and their targets in liver cirrhosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727606/
https://www.ncbi.nlm.nih.gov/pubmed/29250171
http://dx.doi.org/10.3892/ol.2017.7252
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