Cargando…
Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins
The goal of the present study was to determine whether peptidylarginine deiminase PAD2 and PAD4 enzymes are present in Balb/c mouse salivary glands and whether they are able to citrullinate Ro and La ribonucleoproteins. Salivary glands from Balb/c mice were cultured in DMEM and supplemented with one...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727760/ https://www.ncbi.nlm.nih.gov/pubmed/29318161 http://dx.doi.org/10.1155/2017/8959687 |
_version_ | 1783285950231609344 |
---|---|
author | Rodríguez-Rodríguez, Mayra Herrera-Esparza, Rafael Bollain y Goytia, Juan-José Pérez-Pérez, María-Elena Pacheco-Tovar, Deyanira Murillo-Vázquez, Jessica Pacheco-Tovar, Guadalupe Avalos-Díaz, Esperanza |
author_facet | Rodríguez-Rodríguez, Mayra Herrera-Esparza, Rafael Bollain y Goytia, Juan-José Pérez-Pérez, María-Elena Pacheco-Tovar, Deyanira Murillo-Vázquez, Jessica Pacheco-Tovar, Guadalupe Avalos-Díaz, Esperanza |
author_sort | Rodríguez-Rodríguez, Mayra |
collection | PubMed |
description | The goal of the present study was to determine whether peptidylarginine deiminase PAD2 and PAD4 enzymes are present in Balb/c mouse salivary glands and whether they are able to citrullinate Ro and La ribonucleoproteins. Salivary glands from Balb/c mice were cultured in DMEM and supplemented with one of the following stimulants: ATP, LPS, TNF, IFNγ, or IL-6. A control group without stimulant was also evaluated. PAD2, PAD4, citrullinated peptides, Ro60, and La were detected by immunohistochemistry and double immunofluorescence. PAD2 and PAD4 mRNAs and protein expression were detected by qPCR and Western blot analysis. PAD activity was assessed using an antigen capture enzyme-linked immunosorbent assay. LPS, ATP, and TNF triggered PAD2 and PAD4 expression; in contrast, no expression was detected in the control group (p < 0.001). PAD transcription slightly increased in response to stimulation. Additionally, PAD2/4 activity modified the arginine residues of a reporter protein (fibrinogen) in vitro. PADs citrullinated Ro60 and La ribonucleoproteins in vivo. Molecular stimulants induced apoptosis in ductal cells and the externalization of Ro60 and La ribonucleoproteins onto apoptotic membranes. PAD enzymes citrullinate Ro and La ribonucleoproteins, and this experimental approach may facilitate our understanding of the role of posttranslational modifications in the pathophysiology of Sjögren's syndrome. |
format | Online Article Text |
id | pubmed-5727760 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-57277602018-01-09 Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins Rodríguez-Rodríguez, Mayra Herrera-Esparza, Rafael Bollain y Goytia, Juan-José Pérez-Pérez, María-Elena Pacheco-Tovar, Deyanira Murillo-Vázquez, Jessica Pacheco-Tovar, Guadalupe Avalos-Díaz, Esperanza J Immunol Res Research Article The goal of the present study was to determine whether peptidylarginine deiminase PAD2 and PAD4 enzymes are present in Balb/c mouse salivary glands and whether they are able to citrullinate Ro and La ribonucleoproteins. Salivary glands from Balb/c mice were cultured in DMEM and supplemented with one of the following stimulants: ATP, LPS, TNF, IFNγ, or IL-6. A control group without stimulant was also evaluated. PAD2, PAD4, citrullinated peptides, Ro60, and La were detected by immunohistochemistry and double immunofluorescence. PAD2 and PAD4 mRNAs and protein expression were detected by qPCR and Western blot analysis. PAD activity was assessed using an antigen capture enzyme-linked immunosorbent assay. LPS, ATP, and TNF triggered PAD2 and PAD4 expression; in contrast, no expression was detected in the control group (p < 0.001). PAD transcription slightly increased in response to stimulation. Additionally, PAD2/4 activity modified the arginine residues of a reporter protein (fibrinogen) in vitro. PADs citrullinated Ro60 and La ribonucleoproteins in vivo. Molecular stimulants induced apoptosis in ductal cells and the externalization of Ro60 and La ribonucleoproteins onto apoptotic membranes. PAD enzymes citrullinate Ro and La ribonucleoproteins, and this experimental approach may facilitate our understanding of the role of posttranslational modifications in the pathophysiology of Sjögren's syndrome. Hindawi 2017 2017-11-26 /pmc/articles/PMC5727760/ /pubmed/29318161 http://dx.doi.org/10.1155/2017/8959687 Text en Copyright © 2017 Mayra Rodríguez-Rodríguez et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Rodríguez-Rodríguez, Mayra Herrera-Esparza, Rafael Bollain y Goytia, Juan-José Pérez-Pérez, María-Elena Pacheco-Tovar, Deyanira Murillo-Vázquez, Jessica Pacheco-Tovar, Guadalupe Avalos-Díaz, Esperanza Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins |
title | Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins |
title_full | Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins |
title_fullStr | Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins |
title_full_unstemmed | Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins |
title_short | Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins |
title_sort | activation of peptidylarginine deiminase in the salivary glands of balb/c mice drives the citrullination of ro and la ribonucleoproteins |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727760/ https://www.ncbi.nlm.nih.gov/pubmed/29318161 http://dx.doi.org/10.1155/2017/8959687 |
work_keys_str_mv | AT rodriguezrodriguezmayra activationofpeptidylargininedeiminaseinthesalivaryglandsofbalbcmicedrivesthecitrullinationofroandlaribonucleoproteins AT herreraesparzarafael activationofpeptidylargininedeiminaseinthesalivaryglandsofbalbcmicedrivesthecitrullinationofroandlaribonucleoproteins AT bollainygoytiajuanjose activationofpeptidylargininedeiminaseinthesalivaryglandsofbalbcmicedrivesthecitrullinationofroandlaribonucleoproteins AT perezperezmariaelena activationofpeptidylargininedeiminaseinthesalivaryglandsofbalbcmicedrivesthecitrullinationofroandlaribonucleoproteins AT pachecotovardeyanira activationofpeptidylargininedeiminaseinthesalivaryglandsofbalbcmicedrivesthecitrullinationofroandlaribonucleoproteins AT murillovazquezjessica activationofpeptidylargininedeiminaseinthesalivaryglandsofbalbcmicedrivesthecitrullinationofroandlaribonucleoproteins AT pachecotovarguadalupe activationofpeptidylargininedeiminaseinthesalivaryglandsofbalbcmicedrivesthecitrullinationofroandlaribonucleoproteins AT avalosdiazesperanza activationofpeptidylargininedeiminaseinthesalivaryglandsofbalbcmicedrivesthecitrullinationofroandlaribonucleoproteins |