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Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins

The goal of the present study was to determine whether peptidylarginine deiminase PAD2 and PAD4 enzymes are present in Balb/c mouse salivary glands and whether they are able to citrullinate Ro and La ribonucleoproteins. Salivary glands from Balb/c mice were cultured in DMEM and supplemented with one...

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Autores principales: Rodríguez-Rodríguez, Mayra, Herrera-Esparza, Rafael, Bollain y Goytia, Juan-José, Pérez-Pérez, María-Elena, Pacheco-Tovar, Deyanira, Murillo-Vázquez, Jessica, Pacheco-Tovar, Guadalupe, Avalos-Díaz, Esperanza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727760/
https://www.ncbi.nlm.nih.gov/pubmed/29318161
http://dx.doi.org/10.1155/2017/8959687
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author Rodríguez-Rodríguez, Mayra
Herrera-Esparza, Rafael
Bollain y Goytia, Juan-José
Pérez-Pérez, María-Elena
Pacheco-Tovar, Deyanira
Murillo-Vázquez, Jessica
Pacheco-Tovar, Guadalupe
Avalos-Díaz, Esperanza
author_facet Rodríguez-Rodríguez, Mayra
Herrera-Esparza, Rafael
Bollain y Goytia, Juan-José
Pérez-Pérez, María-Elena
Pacheco-Tovar, Deyanira
Murillo-Vázquez, Jessica
Pacheco-Tovar, Guadalupe
Avalos-Díaz, Esperanza
author_sort Rodríguez-Rodríguez, Mayra
collection PubMed
description The goal of the present study was to determine whether peptidylarginine deiminase PAD2 and PAD4 enzymes are present in Balb/c mouse salivary glands and whether they are able to citrullinate Ro and La ribonucleoproteins. Salivary glands from Balb/c mice were cultured in DMEM and supplemented with one of the following stimulants: ATP, LPS, TNF, IFNγ, or IL-6. A control group without stimulant was also evaluated. PAD2, PAD4, citrullinated peptides, Ro60, and La were detected by immunohistochemistry and double immunofluorescence. PAD2 and PAD4 mRNAs and protein expression were detected by qPCR and Western blot analysis. PAD activity was assessed using an antigen capture enzyme-linked immunosorbent assay. LPS, ATP, and TNF triggered PAD2 and PAD4 expression; in contrast, no expression was detected in the control group (p < 0.001). PAD transcription slightly increased in response to stimulation. Additionally, PAD2/4 activity modified the arginine residues of a reporter protein (fibrinogen) in vitro. PADs citrullinated Ro60 and La ribonucleoproteins in vivo. Molecular stimulants induced apoptosis in ductal cells and the externalization of Ro60 and La ribonucleoproteins onto apoptotic membranes. PAD enzymes citrullinate Ro and La ribonucleoproteins, and this experimental approach may facilitate our understanding of the role of posttranslational modifications in the pathophysiology of Sjögren's syndrome.
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spelling pubmed-57277602018-01-09 Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins Rodríguez-Rodríguez, Mayra Herrera-Esparza, Rafael Bollain y Goytia, Juan-José Pérez-Pérez, María-Elena Pacheco-Tovar, Deyanira Murillo-Vázquez, Jessica Pacheco-Tovar, Guadalupe Avalos-Díaz, Esperanza J Immunol Res Research Article The goal of the present study was to determine whether peptidylarginine deiminase PAD2 and PAD4 enzymes are present in Balb/c mouse salivary glands and whether they are able to citrullinate Ro and La ribonucleoproteins. Salivary glands from Balb/c mice were cultured in DMEM and supplemented with one of the following stimulants: ATP, LPS, TNF, IFNγ, or IL-6. A control group without stimulant was also evaluated. PAD2, PAD4, citrullinated peptides, Ro60, and La were detected by immunohistochemistry and double immunofluorescence. PAD2 and PAD4 mRNAs and protein expression were detected by qPCR and Western blot analysis. PAD activity was assessed using an antigen capture enzyme-linked immunosorbent assay. LPS, ATP, and TNF triggered PAD2 and PAD4 expression; in contrast, no expression was detected in the control group (p < 0.001). PAD transcription slightly increased in response to stimulation. Additionally, PAD2/4 activity modified the arginine residues of a reporter protein (fibrinogen) in vitro. PADs citrullinated Ro60 and La ribonucleoproteins in vivo. Molecular stimulants induced apoptosis in ductal cells and the externalization of Ro60 and La ribonucleoproteins onto apoptotic membranes. PAD enzymes citrullinate Ro and La ribonucleoproteins, and this experimental approach may facilitate our understanding of the role of posttranslational modifications in the pathophysiology of Sjögren's syndrome. Hindawi 2017 2017-11-26 /pmc/articles/PMC5727760/ /pubmed/29318161 http://dx.doi.org/10.1155/2017/8959687 Text en Copyright © 2017 Mayra Rodríguez-Rodríguez et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rodríguez-Rodríguez, Mayra
Herrera-Esparza, Rafael
Bollain y Goytia, Juan-José
Pérez-Pérez, María-Elena
Pacheco-Tovar, Deyanira
Murillo-Vázquez, Jessica
Pacheco-Tovar, Guadalupe
Avalos-Díaz, Esperanza
Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins
title Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins
title_full Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins
title_fullStr Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins
title_full_unstemmed Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins
title_short Activation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteins
title_sort activation of peptidylarginine deiminase in the salivary glands of balb/c mice drives the citrullination of ro and la ribonucleoproteins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727760/
https://www.ncbi.nlm.nih.gov/pubmed/29318161
http://dx.doi.org/10.1155/2017/8959687
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