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Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)

BACKGROUND: Transcriptomic profiling of ex vivo cultured human limbal epithelial stem cells (hLESCs) will foster better understanding of corneal physiology and novel treatment paradigms to limbal stem cell deficiency (LSCD). However, currently such profiling studies are hampered due to difficulties...

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Detalles Bibliográficos
Autores principales:	Liu, Lei, Nielsen, Frederik Mølgaard, Riis, Simone Elkjær, Emmersen, Jeppe, Fink, Trine, Hjortdal, Jesper Østergaard, Bath, Chris, Zachar, Vladimir
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727887/ https://www.ncbi.nlm.nih.gov/pubmed/29255379 http://dx.doi.org/10.1186/s12575-017-0065-2

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