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Genome-wide analysis of Atlantic salmon (Salmo salar) mucin genes and their role as biomarkers

The aim of this study was to identify potential mucin genes in the Atlantic salmon genome and evaluate tissue-specific distribution and transcriptional regulation in response to aquaculture-relevant stress conditions in post-smolts. Seven secreted gel-forming mucin genes were identified based on sev...

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Autores principales: Sveen, Lene Rydal, Grammes, Fabian Thomas, Ytteborg, Elisabeth, Takle, Harald, Jørgensen, Sven Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5728529/
https://www.ncbi.nlm.nih.gov/pubmed/29236729
http://dx.doi.org/10.1371/journal.pone.0189103
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author Sveen, Lene Rydal
Grammes, Fabian Thomas
Ytteborg, Elisabeth
Takle, Harald
Jørgensen, Sven Martin
author_facet Sveen, Lene Rydal
Grammes, Fabian Thomas
Ytteborg, Elisabeth
Takle, Harald
Jørgensen, Sven Martin
author_sort Sveen, Lene Rydal
collection PubMed
description The aim of this study was to identify potential mucin genes in the Atlantic salmon genome and evaluate tissue-specific distribution and transcriptional regulation in response to aquaculture-relevant stress conditions in post-smolts. Seven secreted gel-forming mucin genes were identified based on several layers of evidence; annotation, transcription, phylogeny and domain structure. Two genes were annotated as muc2 and five genes as muc5. The muc2 genes were predominantly transcribed in the intestinal region while the different genes in the muc5 family were mainly transcribed in either skin, gill or pyloric caeca. In order to investigate transcriptional regulation of mucins during stress conditions, two controlled experiments were conducted. In the first experiment, handling stress induced mucin transcription in the gill, while transcription decreased in the skin and intestine. In the second experiment, long term intensive rearing conditions (fish biomass ~125 kg/m(3)) interrupted by additional confinement led to increased transcription of mucin genes in the skin at one, seven and fourteen days post-confinement.
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spelling pubmed-57285292017-12-22 Genome-wide analysis of Atlantic salmon (Salmo salar) mucin genes and their role as biomarkers Sveen, Lene Rydal Grammes, Fabian Thomas Ytteborg, Elisabeth Takle, Harald Jørgensen, Sven Martin PLoS One Research Article The aim of this study was to identify potential mucin genes in the Atlantic salmon genome and evaluate tissue-specific distribution and transcriptional regulation in response to aquaculture-relevant stress conditions in post-smolts. Seven secreted gel-forming mucin genes were identified based on several layers of evidence; annotation, transcription, phylogeny and domain structure. Two genes were annotated as muc2 and five genes as muc5. The muc2 genes were predominantly transcribed in the intestinal region while the different genes in the muc5 family were mainly transcribed in either skin, gill or pyloric caeca. In order to investigate transcriptional regulation of mucins during stress conditions, two controlled experiments were conducted. In the first experiment, handling stress induced mucin transcription in the gill, while transcription decreased in the skin and intestine. In the second experiment, long term intensive rearing conditions (fish biomass ~125 kg/m(3)) interrupted by additional confinement led to increased transcription of mucin genes in the skin at one, seven and fourteen days post-confinement. Public Library of Science 2017-12-13 /pmc/articles/PMC5728529/ /pubmed/29236729 http://dx.doi.org/10.1371/journal.pone.0189103 Text en © 2017 Sveen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sveen, Lene Rydal
Grammes, Fabian Thomas
Ytteborg, Elisabeth
Takle, Harald
Jørgensen, Sven Martin
Genome-wide analysis of Atlantic salmon (Salmo salar) mucin genes and their role as biomarkers
title Genome-wide analysis of Atlantic salmon (Salmo salar) mucin genes and their role as biomarkers
title_full Genome-wide analysis of Atlantic salmon (Salmo salar) mucin genes and their role as biomarkers
title_fullStr Genome-wide analysis of Atlantic salmon (Salmo salar) mucin genes and their role as biomarkers
title_full_unstemmed Genome-wide analysis of Atlantic salmon (Salmo salar) mucin genes and their role as biomarkers
title_short Genome-wide analysis of Atlantic salmon (Salmo salar) mucin genes and their role as biomarkers
title_sort genome-wide analysis of atlantic salmon (salmo salar) mucin genes and their role as biomarkers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5728529/
https://www.ncbi.nlm.nih.gov/pubmed/29236729
http://dx.doi.org/10.1371/journal.pone.0189103
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