Cargando…
The role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves
Glycoalkaloids are toxic compounds that are synthesized by many Solanum species. Glycoalkaloid biosynthesis is influenced by plant genetic and environmental conditions. Although many studies have shown that light is an important factor affecting glycoalkaloid biosynthesis, the specific mechanism is...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5728552/ https://www.ncbi.nlm.nih.gov/pubmed/29236780 http://dx.doi.org/10.1371/journal.pone.0189481 |
_version_ | 1783286043952283648 |
---|---|
author | Wang, Cui-Cui Sulli, Maria Fu, Da-Qi |
author_facet | Wang, Cui-Cui Sulli, Maria Fu, Da-Qi |
author_sort | Wang, Cui-Cui |
collection | PubMed |
description | Glycoalkaloids are toxic compounds that are synthesized by many Solanum species. Glycoalkaloid biosynthesis is influenced by plant genetic and environmental conditions. Although many studies have shown that light is an important factor affecting glycoalkaloid biosynthesis, the specific mechanism is currently unknown. Chlorophyll and carotenoid biosynthesis depend on light signal transduction and share some intermediate metabolites with the glycoalkaloid biosynthetic pathway. Here, we used virus-induced gene silencing to silence genes encoding phytoene desaturase (PDS) and magnesium chelatase (CHLI and CHLH) to reduce chlorophyll and carotenoid levels in eggplant leaves. Quantification of carotenoid and chlorophyll levels is analyzed by LC/PDA/APCI/MS and semipolar metabolite profiling by LC/HESI/MS. Notably, the resulting lines showed decreases in glycoalkaloid production. We further found that the expression of some genes involved in the production of glycoalkaloids and other metabolites were suppressed in these silenced lines. Our results indicate that photosynthetic pigment accumulation affects steroidal glycoalkaloid biosynthesis in eggplant leaves. This finding lays the foundation for reducing the levels of endogenous antinutritional compounds in crops. |
format | Online Article Text |
id | pubmed-5728552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57285522017-12-22 The role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves Wang, Cui-Cui Sulli, Maria Fu, Da-Qi PLoS One Research Article Glycoalkaloids are toxic compounds that are synthesized by many Solanum species. Glycoalkaloid biosynthesis is influenced by plant genetic and environmental conditions. Although many studies have shown that light is an important factor affecting glycoalkaloid biosynthesis, the specific mechanism is currently unknown. Chlorophyll and carotenoid biosynthesis depend on light signal transduction and share some intermediate metabolites with the glycoalkaloid biosynthetic pathway. Here, we used virus-induced gene silencing to silence genes encoding phytoene desaturase (PDS) and magnesium chelatase (CHLI and CHLH) to reduce chlorophyll and carotenoid levels in eggplant leaves. Quantification of carotenoid and chlorophyll levels is analyzed by LC/PDA/APCI/MS and semipolar metabolite profiling by LC/HESI/MS. Notably, the resulting lines showed decreases in glycoalkaloid production. We further found that the expression of some genes involved in the production of glycoalkaloids and other metabolites were suppressed in these silenced lines. Our results indicate that photosynthetic pigment accumulation affects steroidal glycoalkaloid biosynthesis in eggplant leaves. This finding lays the foundation for reducing the levels of endogenous antinutritional compounds in crops. Public Library of Science 2017-12-13 /pmc/articles/PMC5728552/ /pubmed/29236780 http://dx.doi.org/10.1371/journal.pone.0189481 Text en © 2017 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Cui-Cui Sulli, Maria Fu, Da-Qi The role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves |
title | The role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves |
title_full | The role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves |
title_fullStr | The role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves |
title_full_unstemmed | The role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves |
title_short | The role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves |
title_sort | role of phytochromes in regulating biosynthesis of sterol glycoalkaloid in eggplant leaves |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5728552/ https://www.ncbi.nlm.nih.gov/pubmed/29236780 http://dx.doi.org/10.1371/journal.pone.0189481 |
work_keys_str_mv | AT wangcuicui theroleofphytochromesinregulatingbiosynthesisofsterolglycoalkaloidineggplantleaves AT sullimaria theroleofphytochromesinregulatingbiosynthesisofsterolglycoalkaloidineggplantleaves AT fudaqi theroleofphytochromesinregulatingbiosynthesisofsterolglycoalkaloidineggplantleaves AT wangcuicui roleofphytochromesinregulatingbiosynthesisofsterolglycoalkaloidineggplantleaves AT sullimaria roleofphytochromesinregulatingbiosynthesisofsterolglycoalkaloidineggplantleaves AT fudaqi roleofphytochromesinregulatingbiosynthesisofsterolglycoalkaloidineggplantleaves |