Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016
Noroviruses are the leading cause of acute gastroenteritis (AGE) in all age groups worldwide. Despite the high genetic diversity of noroviruses, most AGE outbreaks are caused by a single norovirus genotype: GII.4. Since 1995, several different variants of norovirus GII.4 have been associated with pa...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5728567/ https://www.ncbi.nlm.nih.gov/pubmed/29236779 http://dx.doi.org/10.1371/journal.pone.0189504 |
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author | Barreira, Débora Maria Pires Gonçalves Fumian, Túlio Machado Tonini, Marco André Loureiro Volpini, Lays Paula Bondi Santos, Rodrigo Pratte Ribeiro, Anézia Lima Chaves Leite, José Paulo Gagliardi Souza, Márcia Terezinha Baroni de Moraes e Brasil, Patrícia da Cunha, Denise Cotrim Miagostovich, Marize Pereira Spano, Liliana Cruz |
author_facet | Barreira, Débora Maria Pires Gonçalves Fumian, Túlio Machado Tonini, Marco André Loureiro Volpini, Lays Paula Bondi Santos, Rodrigo Pratte Ribeiro, Anézia Lima Chaves Leite, José Paulo Gagliardi Souza, Márcia Terezinha Baroni de Moraes e Brasil, Patrícia da Cunha, Denise Cotrim Miagostovich, Marize Pereira Spano, Liliana Cruz |
author_sort | Barreira, Débora Maria Pires Gonçalves |
collection | PubMed |
description | Noroviruses are the leading cause of acute gastroenteritis (AGE) in all age groups worldwide. Despite the high genetic diversity of noroviruses, most AGE outbreaks are caused by a single norovirus genotype: GII.4. Since 1995, several different variants of norovirus GII.4 have been associated with pandemics, with each variant circulating for 3 to 8 years. The Sydney_2012 variant was first reported in Australia and then in other countries. A new variant, GII.P16-GII.4, was recently described in Japan and South Korea and then in the USA, France, Germany and England. In our study, 190 faecal specimens were collected from children admitted to a paediatric hospital and a public health facility during a surveillance study of sporadic cases of AGE conducted between January 2015 and July 2016. The norovirus was detected by RT-qPCR in 51 samples (26.8%), and in 37 of them (72.5%), the ORF1-2 junction was successfully sequenced. The new recombinant GII.P16-GII.4 Sydney was revealed for the first time in Brazil in 2016 and predominated among other strains (9 GII.Pe-GII.4, 3 GII.P17-GII.17, 1 GII.Pg-GII.1, 1 GII.P16-GII.3 and 1 GII.PNA-GII.4). The epidemiological significance of this new recombinant is still unknown, but continuous surveillance studies may evaluate its impact on the population, its potential to replace the first recombinant GII.Pe-GII.4 Sydney 2012 variant, and the emergence of new recombinant forms of GII.P16. |
format | Online Article Text |
id | pubmed-5728567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57285672017-12-22 Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016 Barreira, Débora Maria Pires Gonçalves Fumian, Túlio Machado Tonini, Marco André Loureiro Volpini, Lays Paula Bondi Santos, Rodrigo Pratte Ribeiro, Anézia Lima Chaves Leite, José Paulo Gagliardi Souza, Márcia Terezinha Baroni de Moraes e Brasil, Patrícia da Cunha, Denise Cotrim Miagostovich, Marize Pereira Spano, Liliana Cruz PLoS One Research Article Noroviruses are the leading cause of acute gastroenteritis (AGE) in all age groups worldwide. Despite the high genetic diversity of noroviruses, most AGE outbreaks are caused by a single norovirus genotype: GII.4. Since 1995, several different variants of norovirus GII.4 have been associated with pandemics, with each variant circulating for 3 to 8 years. The Sydney_2012 variant was first reported in Australia and then in other countries. A new variant, GII.P16-GII.4, was recently described in Japan and South Korea and then in the USA, France, Germany and England. In our study, 190 faecal specimens were collected from children admitted to a paediatric hospital and a public health facility during a surveillance study of sporadic cases of AGE conducted between January 2015 and July 2016. The norovirus was detected by RT-qPCR in 51 samples (26.8%), and in 37 of them (72.5%), the ORF1-2 junction was successfully sequenced. The new recombinant GII.P16-GII.4 Sydney was revealed for the first time in Brazil in 2016 and predominated among other strains (9 GII.Pe-GII.4, 3 GII.P17-GII.17, 1 GII.Pg-GII.1, 1 GII.P16-GII.3 and 1 GII.PNA-GII.4). The epidemiological significance of this new recombinant is still unknown, but continuous surveillance studies may evaluate its impact on the population, its potential to replace the first recombinant GII.Pe-GII.4 Sydney 2012 variant, and the emergence of new recombinant forms of GII.P16. Public Library of Science 2017-12-13 /pmc/articles/PMC5728567/ /pubmed/29236779 http://dx.doi.org/10.1371/journal.pone.0189504 Text en © 2017 Barreira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Barreira, Débora Maria Pires Gonçalves Fumian, Túlio Machado Tonini, Marco André Loureiro Volpini, Lays Paula Bondi Santos, Rodrigo Pratte Ribeiro, Anézia Lima Chaves Leite, José Paulo Gagliardi Souza, Márcia Terezinha Baroni de Moraes e Brasil, Patrícia da Cunha, Denise Cotrim Miagostovich, Marize Pereira Spano, Liliana Cruz Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016 |
title | Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016 |
title_full | Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016 |
title_fullStr | Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016 |
title_full_unstemmed | Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016 |
title_short | Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016 |
title_sort | detection and molecular characterization of the novel recombinant norovirus gii.p16-gii.4 sydney in southeastern brazil in 2016 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5728567/ https://www.ncbi.nlm.nih.gov/pubmed/29236779 http://dx.doi.org/10.1371/journal.pone.0189504 |
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