Cargando…
Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues
BACKGROUND: To investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for translational activity. Furthermore, we developed an oligonucleotide based microa...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729415/ https://www.ncbi.nlm.nih.gov/pubmed/29255478 http://dx.doi.org/10.1186/s13007-017-0257-4 |
_version_ | 1783286190219198464 |
---|---|
author | Hameed, Muhammad Waqar Juszczak, Ilona Bock, Ralph van Dongen, Joost Thomas |
author_facet | Hameed, Muhammad Waqar Juszczak, Ilona Bock, Ralph van Dongen, Joost Thomas |
author_sort | Hameed, Muhammad Waqar |
collection | PubMed |
description | BACKGROUND: To investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for translational activity. Furthermore, we developed an oligonucleotide based microarray platform to determine the level of Nicotiana tabacum and Arabidopsis thaliana mitochondrial mRNA. RESULTS: Microarray analysis of free and polysomal mRNAs was used to characterize differences in the levels of free transcripts and ribosome-bound mRNAs in various organs of tobacco plants. We have observed higher mitochondrial transcript levels in young leaves, flowers and floral buds as compared to fully expanded leaves and roots. A similar pattern of abundance was observed for ribosome-bound mitochondrial mRNAs in these tissues. However, the accumulation of the mitochondrial protein COX2 was found to be inversely related to that of its ribosome-bound mRNA. CONCLUSIONS: Our results indicate that the association of mitochondrial mRNAs to ribosomes is largely determined by the total transcript level of a gene. However, at least for Cox2, we demonstrated that the level of ribosome-bound mRNA is not reflected by the amount of COX2 protein. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13007-017-0257-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5729415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57294152017-12-18 Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues Hameed, Muhammad Waqar Juszczak, Ilona Bock, Ralph van Dongen, Joost Thomas Plant Methods Methodology BACKGROUND: To investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for translational activity. Furthermore, we developed an oligonucleotide based microarray platform to determine the level of Nicotiana tabacum and Arabidopsis thaliana mitochondrial mRNA. RESULTS: Microarray analysis of free and polysomal mRNAs was used to characterize differences in the levels of free transcripts and ribosome-bound mRNAs in various organs of tobacco plants. We have observed higher mitochondrial transcript levels in young leaves, flowers and floral buds as compared to fully expanded leaves and roots. A similar pattern of abundance was observed for ribosome-bound mitochondrial mRNAs in these tissues. However, the accumulation of the mitochondrial protein COX2 was found to be inversely related to that of its ribosome-bound mRNA. CONCLUSIONS: Our results indicate that the association of mitochondrial mRNAs to ribosomes is largely determined by the total transcript level of a gene. However, at least for Cox2, we demonstrated that the level of ribosome-bound mRNA is not reflected by the amount of COX2 protein. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13007-017-0257-4) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-13 /pmc/articles/PMC5729415/ /pubmed/29255478 http://dx.doi.org/10.1186/s13007-017-0257-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Methodology Hameed, Muhammad Waqar Juszczak, Ilona Bock, Ralph van Dongen, Joost Thomas Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues |
title | Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues |
title_full | Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues |
title_fullStr | Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues |
title_full_unstemmed | Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues |
title_short | Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues |
title_sort | comparison of mitochondrial gene expression and polysome loading in different tobacco tissues |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729415/ https://www.ncbi.nlm.nih.gov/pubmed/29255478 http://dx.doi.org/10.1186/s13007-017-0257-4 |
work_keys_str_mv | AT hameedmuhammadwaqar comparisonofmitochondrialgeneexpressionandpolysomeloadingindifferenttobaccotissues AT juszczakilona comparisonofmitochondrialgeneexpressionandpolysomeloadingindifferenttobaccotissues AT bockralph comparisonofmitochondrialgeneexpressionandpolysomeloadingindifferenttobaccotissues AT vandongenjoostthomas comparisonofmitochondrialgeneexpressionandpolysomeloadingindifferenttobaccotissues |