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A positive-feedback loop between tumour infiltrating activated Treg cells and type 2-skewed macrophages is essential for progression of laryngeal squamous cell carcinoma

BACKGROUND: Foxp3(+) regulatory T (Treg) cells and M2 macrophages are associated with increased tumour progression. However, the interaction between Treg cells and M2 macrophages remains unclear. METHODS: The expression of FoxP3 and CD163 was detected by immunohistochemistry in 65 cases of laryngeal...

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Autores principales: Sun, Wei, Wei, Fan-Qin, Li, Wei-Jin, Wei, Jia-Wei, Zhong, Hua, Wen, Yi-Hui, Lei, Wen-Bin, Chen, Lin, Li, Hang, Lin, Han-Qing, Iqbal, Muhammad, Wen, Wei-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729431/
https://www.ncbi.nlm.nih.gov/pubmed/28949956
http://dx.doi.org/10.1038/bjc.2017.329
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author Sun, Wei
Wei, Fan-Qin
Li, Wei-Jin
Wei, Jia-Wei
Zhong, Hua
Wen, Yi-Hui
Lei, Wen-Bin
Chen, Lin
Li, Hang
Lin, Han-Qing
Iqbal, Muhammad
Wen, Wei-Ping
author_facet Sun, Wei
Wei, Fan-Qin
Li, Wei-Jin
Wei, Jia-Wei
Zhong, Hua
Wen, Yi-Hui
Lei, Wen-Bin
Chen, Lin
Li, Hang
Lin, Han-Qing
Iqbal, Muhammad
Wen, Wei-Ping
author_sort Sun, Wei
collection PubMed
description BACKGROUND: Foxp3(+) regulatory T (Treg) cells and M2 macrophages are associated with increased tumour progression. However, the interaction between Treg cells and M2 macrophages remains unclear. METHODS: The expression of FoxP3 and CD163 was detected by immunohistochemistry in 65 cases of laryngeal squamous cell carcinoma (LSCC). In vitro, the generation of activated Treg (aTreg) cells and M2 macrophages by interactions with their precursor cells were analysed by flow cytometry and ELISA. In vivo, the antitumour effects were assessed by combined targeting aTreg cells and M2 macrophages, and intratumoural immunocytes were analysed by flow cytometry. RESULTS: In LSCC tissue, accumulation of aTreg cells and M2 macrophages predicted a poor prognosis and were positively associated with each other. In vitro, aTreg cells were induced from CD4(+)CD25(−) T cells by cancer cell-activated M2-like macrophages. Consequently, these aTreg cells skewed the differentiation of monocytes towards an M2-like phenotype, thereby forming a positive-feedback loop. Combined targeting aTreg cells and M2 macrophages led to potent antitumour immunity in vivo. CONCLUSIONS: The positive-feedback loop between aTreg cells and M2 macrophages is essential to maintain or promote immunosuppression in the tumour microenvironment and may be a potential therapeutic target to inhibit tumour progression.
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spelling pubmed-57294312018-11-21 A positive-feedback loop between tumour infiltrating activated Treg cells and type 2-skewed macrophages is essential for progression of laryngeal squamous cell carcinoma Sun, Wei Wei, Fan-Qin Li, Wei-Jin Wei, Jia-Wei Zhong, Hua Wen, Yi-Hui Lei, Wen-Bin Chen, Lin Li, Hang Lin, Han-Qing Iqbal, Muhammad Wen, Wei-Ping Br J Cancer Translational Therapeutics BACKGROUND: Foxp3(+) regulatory T (Treg) cells and M2 macrophages are associated with increased tumour progression. However, the interaction between Treg cells and M2 macrophages remains unclear. METHODS: The expression of FoxP3 and CD163 was detected by immunohistochemistry in 65 cases of laryngeal squamous cell carcinoma (LSCC). In vitro, the generation of activated Treg (aTreg) cells and M2 macrophages by interactions with their precursor cells were analysed by flow cytometry and ELISA. In vivo, the antitumour effects were assessed by combined targeting aTreg cells and M2 macrophages, and intratumoural immunocytes were analysed by flow cytometry. RESULTS: In LSCC tissue, accumulation of aTreg cells and M2 macrophages predicted a poor prognosis and were positively associated with each other. In vitro, aTreg cells were induced from CD4(+)CD25(−) T cells by cancer cell-activated M2-like macrophages. Consequently, these aTreg cells skewed the differentiation of monocytes towards an M2-like phenotype, thereby forming a positive-feedback loop. Combined targeting aTreg cells and M2 macrophages led to potent antitumour immunity in vivo. CONCLUSIONS: The positive-feedback loop between aTreg cells and M2 macrophages is essential to maintain or promote immunosuppression in the tumour microenvironment and may be a potential therapeutic target to inhibit tumour progression. Nature Publishing Group 2017-11-21 2017-09-26 /pmc/articles/PMC5729431/ /pubmed/28949956 http://dx.doi.org/10.1038/bjc.2017.329 Text en Copyright © 2017 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/4.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Translational Therapeutics
Sun, Wei
Wei, Fan-Qin
Li, Wei-Jin
Wei, Jia-Wei
Zhong, Hua
Wen, Yi-Hui
Lei, Wen-Bin
Chen, Lin
Li, Hang
Lin, Han-Qing
Iqbal, Muhammad
Wen, Wei-Ping
A positive-feedback loop between tumour infiltrating activated Treg cells and type 2-skewed macrophages is essential for progression of laryngeal squamous cell carcinoma
title A positive-feedback loop between tumour infiltrating activated Treg cells and type 2-skewed macrophages is essential for progression of laryngeal squamous cell carcinoma
title_full A positive-feedback loop between tumour infiltrating activated Treg cells and type 2-skewed macrophages is essential for progression of laryngeal squamous cell carcinoma
title_fullStr A positive-feedback loop between tumour infiltrating activated Treg cells and type 2-skewed macrophages is essential for progression of laryngeal squamous cell carcinoma
title_full_unstemmed A positive-feedback loop between tumour infiltrating activated Treg cells and type 2-skewed macrophages is essential for progression of laryngeal squamous cell carcinoma
title_short A positive-feedback loop between tumour infiltrating activated Treg cells and type 2-skewed macrophages is essential for progression of laryngeal squamous cell carcinoma
title_sort positive-feedback loop between tumour infiltrating activated treg cells and type 2-skewed macrophages is essential for progression of laryngeal squamous cell carcinoma
topic Translational Therapeutics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729431/
https://www.ncbi.nlm.nih.gov/pubmed/28949956
http://dx.doi.org/10.1038/bjc.2017.329
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