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Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation

BACKGROUND: Although previous reports have revealed the therapeutic potential of stem cell transplantation in diabetic polyneuropathy, the effects of cell transplantation on long-term diabetic polyneuropathy have not been investigated. In this study, we investigated whether the transplantation of de...

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Autores principales: Omi, Maiko, Hata, Masaki, Nakamura, Nobuhisa, Miyabe, Megumi, Ozawa, Shogo, Nukada, Hitoshi, Tsukamoto, Masami, Sango, Kazunori, Himeno, Tatsuhito, Kamiya, Hideki, Nakamura, Jiro, Takebe, Jun, Matsubara, Tatsuaki, Naruse, Keiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729514/
https://www.ncbi.nlm.nih.gov/pubmed/29237486
http://dx.doi.org/10.1186/s13287-017-0729-5
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author Omi, Maiko
Hata, Masaki
Nakamura, Nobuhisa
Miyabe, Megumi
Ozawa, Shogo
Nukada, Hitoshi
Tsukamoto, Masami
Sango, Kazunori
Himeno, Tatsuhito
Kamiya, Hideki
Nakamura, Jiro
Takebe, Jun
Matsubara, Tatsuaki
Naruse, Keiko
author_facet Omi, Maiko
Hata, Masaki
Nakamura, Nobuhisa
Miyabe, Megumi
Ozawa, Shogo
Nukada, Hitoshi
Tsukamoto, Masami
Sango, Kazunori
Himeno, Tatsuhito
Kamiya, Hideki
Nakamura, Jiro
Takebe, Jun
Matsubara, Tatsuaki
Naruse, Keiko
author_sort Omi, Maiko
collection PubMed
description BACKGROUND: Although previous reports have revealed the therapeutic potential of stem cell transplantation in diabetic polyneuropathy, the effects of cell transplantation on long-term diabetic polyneuropathy have not been investigated. In this study, we investigated whether the transplantation of dental pulp stem cells (DPSCs) ameliorated long-term diabetic polyneuropathy in streptozotocin (STZ)-induced diabetic rats. METHODS: Forty-eight weeks after STZ injection, we transplanted DPSCs into the unilateral hindlimb skeletal muscles. Four weeks after DPSC transplantation (i.e., 52 weeks after STZ injection) the effects of DPSC transplantation on diabetic polyneuropathy were assessed. RESULTS: STZ-induced diabetic rats showed significant reductions in the sciatic motor/sensory nerve conduction velocity, increases in the current perception threshold, and decreases in capillary density in skeletal muscles and intra-epidermal nerve fiber density compared with normal rats, all of which were ameliorated by DPSC transplantation. Furthermore, sural nerve morphometrical analysis revealed that the transplantation of DPSCs significantly increased the myelin thickness and area. DPSC-conditioned media promoted the neurite outgrowth of dorsal root ganglion neurons and increased the viability and myelin-related protein expression of Schwann cells. CONCLUSIONS: These results indicated that the transplantation of DPSCs contributed to the neurophysiological and neuropathological recovery from a long duration of diabetic polyneuropathy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-017-0729-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-57295142017-12-18 Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation Omi, Maiko Hata, Masaki Nakamura, Nobuhisa Miyabe, Megumi Ozawa, Shogo Nukada, Hitoshi Tsukamoto, Masami Sango, Kazunori Himeno, Tatsuhito Kamiya, Hideki Nakamura, Jiro Takebe, Jun Matsubara, Tatsuaki Naruse, Keiko Stem Cell Res Ther Research BACKGROUND: Although previous reports have revealed the therapeutic potential of stem cell transplantation in diabetic polyneuropathy, the effects of cell transplantation on long-term diabetic polyneuropathy have not been investigated. In this study, we investigated whether the transplantation of dental pulp stem cells (DPSCs) ameliorated long-term diabetic polyneuropathy in streptozotocin (STZ)-induced diabetic rats. METHODS: Forty-eight weeks after STZ injection, we transplanted DPSCs into the unilateral hindlimb skeletal muscles. Four weeks after DPSC transplantation (i.e., 52 weeks after STZ injection) the effects of DPSC transplantation on diabetic polyneuropathy were assessed. RESULTS: STZ-induced diabetic rats showed significant reductions in the sciatic motor/sensory nerve conduction velocity, increases in the current perception threshold, and decreases in capillary density in skeletal muscles and intra-epidermal nerve fiber density compared with normal rats, all of which were ameliorated by DPSC transplantation. Furthermore, sural nerve morphometrical analysis revealed that the transplantation of DPSCs significantly increased the myelin thickness and area. DPSC-conditioned media promoted the neurite outgrowth of dorsal root ganglion neurons and increased the viability and myelin-related protein expression of Schwann cells. CONCLUSIONS: These results indicated that the transplantation of DPSCs contributed to the neurophysiological and neuropathological recovery from a long duration of diabetic polyneuropathy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-017-0729-5) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-13 /pmc/articles/PMC5729514/ /pubmed/29237486 http://dx.doi.org/10.1186/s13287-017-0729-5 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Omi, Maiko
Hata, Masaki
Nakamura, Nobuhisa
Miyabe, Megumi
Ozawa, Shogo
Nukada, Hitoshi
Tsukamoto, Masami
Sango, Kazunori
Himeno, Tatsuhito
Kamiya, Hideki
Nakamura, Jiro
Takebe, Jun
Matsubara, Tatsuaki
Naruse, Keiko
Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation
title Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation
title_full Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation
title_fullStr Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation
title_full_unstemmed Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation
title_short Transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation
title_sort transplantation of dental pulp stem cells improves long-term diabetic polyneuropathy together with improvement of nerve morphometrical evaluation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729514/
https://www.ncbi.nlm.nih.gov/pubmed/29237486
http://dx.doi.org/10.1186/s13287-017-0729-5
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