Cargando…
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
BACKGROUND: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. OBJECTIVE: To charact...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Cardiologia - SBC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729779/ https://www.ncbi.nlm.nih.gov/pubmed/29069204 http://dx.doi.org/10.5935/abc.20170151 |
_version_ | 1783286248997126144 |
---|---|
author | Jacobsen, Bruno Barcellos Leopoldo, Ana Paula Lima Cordeiro, Jóctan Pimentel de Campos, Dijon Henrique Salomé do Nascimento, André Ferreira Sugizaki, Mário Mateus Cicogna, Antônio Carlos Padovani, Carlos Roberto Leopoldo, André Soares |
author_facet | Jacobsen, Bruno Barcellos Leopoldo, Ana Paula Lima Cordeiro, Jóctan Pimentel de Campos, Dijon Henrique Salomé do Nascimento, André Ferreira Sugizaki, Mário Mateus Cicogna, Antônio Carlos Padovani, Carlos Roberto Leopoldo, André Soares |
author_sort | Jacobsen, Bruno Barcellos |
collection | PubMed |
description | BACKGROUND: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. OBJECTIVE: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca(2+) handling in short-term exposure to obesity was verified. METHODS: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca(2+) handling proteins were determined by Western Blot. RESULTS: The initial moment of obesity occurred at the 3(rd) week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm(2)/s and HF: 68.0 ± 17.0 g/mm(2)/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca(2+) handling proteins. CONCLUSION: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca(2+) handling. |
format | Online Article Text |
id | pubmed-5729779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Sociedade Brasileira de Cardiologia - SBC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57297792017-12-18 Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity Jacobsen, Bruno Barcellos Leopoldo, Ana Paula Lima Cordeiro, Jóctan Pimentel de Campos, Dijon Henrique Salomé do Nascimento, André Ferreira Sugizaki, Mário Mateus Cicogna, Antônio Carlos Padovani, Carlos Roberto Leopoldo, André Soares Arq Bras Cardiol Original Articles BACKGROUND: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. OBJECTIVE: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca(2+) handling in short-term exposure to obesity was verified. METHODS: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca(2+) handling proteins were determined by Western Blot. RESULTS: The initial moment of obesity occurred at the 3(rd) week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm(2)/s and HF: 68.0 ± 17.0 g/mm(2)/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca(2+) handling proteins. CONCLUSION: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca(2+) handling. Sociedade Brasileira de Cardiologia - SBC 2017-11 /pmc/articles/PMC5729779/ /pubmed/29069204 http://dx.doi.org/10.5935/abc.20170151 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Jacobsen, Bruno Barcellos Leopoldo, Ana Paula Lima Cordeiro, Jóctan Pimentel de Campos, Dijon Henrique Salomé do Nascimento, André Ferreira Sugizaki, Mário Mateus Cicogna, Antônio Carlos Padovani, Carlos Roberto Leopoldo, André Soares Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity |
title | Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the
Initial Moment of Obesity |
title_full | Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the
Initial Moment of Obesity |
title_fullStr | Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the
Initial Moment of Obesity |
title_full_unstemmed | Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the
Initial Moment of Obesity |
title_short | Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the
Initial Moment of Obesity |
title_sort | cardiac, metabolic and molecular profiles of sedentary rats in the
initial moment of obesity |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729779/ https://www.ncbi.nlm.nih.gov/pubmed/29069204 http://dx.doi.org/10.5935/abc.20170151 |
work_keys_str_mv | AT jacobsenbrunobarcellos cardiacmetabolicandmolecularprofilesofsedentaryratsintheinitialmomentofobesity AT leopoldoanapaulalima cardiacmetabolicandmolecularprofilesofsedentaryratsintheinitialmomentofobesity AT cordeirojoctanpimentel cardiacmetabolicandmolecularprofilesofsedentaryratsintheinitialmomentofobesity AT decamposdijonhenriquesalome cardiacmetabolicandmolecularprofilesofsedentaryratsintheinitialmomentofobesity AT donascimentoandreferreira cardiacmetabolicandmolecularprofilesofsedentaryratsintheinitialmomentofobesity AT sugizakimariomateus cardiacmetabolicandmolecularprofilesofsedentaryratsintheinitialmomentofobesity AT cicognaantoniocarlos cardiacmetabolicandmolecularprofilesofsedentaryratsintheinitialmomentofobesity AT padovanicarlosroberto cardiacmetabolicandmolecularprofilesofsedentaryratsintheinitialmomentofobesity AT leopoldoandresoares cardiacmetabolicandmolecularprofilesofsedentaryratsintheinitialmomentofobesity |