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Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity

BACKGROUND: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. OBJECTIVE: To charact...

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Autores principales: Jacobsen, Bruno Barcellos, Leopoldo, Ana Paula Lima, Cordeiro, Jóctan Pimentel, de Campos, Dijon Henrique Salomé, do Nascimento, André Ferreira, Sugizaki, Mário Mateus, Cicogna, Antônio Carlos, Padovani, Carlos Roberto, Leopoldo, André Soares
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Cardiologia - SBC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729779/
https://www.ncbi.nlm.nih.gov/pubmed/29069204
http://dx.doi.org/10.5935/abc.20170151
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author Jacobsen, Bruno Barcellos
Leopoldo, Ana Paula Lima
Cordeiro, Jóctan Pimentel
de Campos, Dijon Henrique Salomé
do Nascimento, André Ferreira
Sugizaki, Mário Mateus
Cicogna, Antônio Carlos
Padovani, Carlos Roberto
Leopoldo, André Soares
author_facet Jacobsen, Bruno Barcellos
Leopoldo, Ana Paula Lima
Cordeiro, Jóctan Pimentel
de Campos, Dijon Henrique Salomé
do Nascimento, André Ferreira
Sugizaki, Mário Mateus
Cicogna, Antônio Carlos
Padovani, Carlos Roberto
Leopoldo, André Soares
author_sort Jacobsen, Bruno Barcellos
collection PubMed
description BACKGROUND: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. OBJECTIVE: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca(2+) handling in short-term exposure to obesity was verified. METHODS: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca(2+) handling proteins were determined by Western Blot. RESULTS: The initial moment of obesity occurred at the 3(rd) week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm(2)/s and HF: 68.0 ± 17.0 g/mm(2)/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca(2+) handling proteins. CONCLUSION: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca(2+) handling.
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spelling pubmed-57297792017-12-18 Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity Jacobsen, Bruno Barcellos Leopoldo, Ana Paula Lima Cordeiro, Jóctan Pimentel de Campos, Dijon Henrique Salomé do Nascimento, André Ferreira Sugizaki, Mário Mateus Cicogna, Antônio Carlos Padovani, Carlos Roberto Leopoldo, André Soares Arq Bras Cardiol Original Articles BACKGROUND: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. OBJECTIVE: To characterize the initial moment of obesity and investigate both metabolic and cardiac parameters. In addition, the role of Ca(2+) handling in short-term exposure to obesity was verified. METHODS: Thirty-day-old male Wistar rats were randomized into two groups (n = 19 each): control (C; standard diet) and high-fat diet (HF, unsaturated high-fat diet). The initial moment of obesity was defined by weekly measurement of body weight (BW) complemented by adiposity index (AI). Cardiac remodelling was assessed by morphological, histological, echocardiographic and papillary muscle analysis. Ca(2+) handling proteins were determined by Western Blot. RESULTS: The initial moment of obesity occurred at the 3(rd) week. Compared with C rats, the HF rats had higher final BW (4%), body fat (20%), AI (14.5%), insulin levels (39.7%), leptin (62.4%) and low-density lipoprotein cholesterol (15.5%) but did not exhibit alterations in systolic blood pressure. Echocardiographic evaluation did not show alterations in cardiac parameters. In the HF group, muscles were observed to increase their +dT/dt (C: 52.6 ± 9.0 g/mm(2)/s and HF: 68.0 ± 17.0 g/mm(2)/s; p < 0.05). In addition, there was no changes in the cardiac expression of Ca(2+) handling proteins. CONCLUSION: The initial moment of obesity promotes alterations to hormonal and lipid profiles without cardiac damage or changes in Ca(2+) handling. Sociedade Brasileira de Cardiologia - SBC 2017-11 /pmc/articles/PMC5729779/ /pubmed/29069204 http://dx.doi.org/10.5935/abc.20170151 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Jacobsen, Bruno Barcellos
Leopoldo, Ana Paula Lima
Cordeiro, Jóctan Pimentel
de Campos, Dijon Henrique Salomé
do Nascimento, André Ferreira
Sugizaki, Mário Mateus
Cicogna, Antônio Carlos
Padovani, Carlos Roberto
Leopoldo, André Soares
Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title_full Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title_fullStr Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title_full_unstemmed Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title_short Cardiac, Metabolic and Molecular Profiles of Sedentary Rats in the Initial Moment of Obesity
title_sort cardiac, metabolic and molecular profiles of sedentary rats in the initial moment of obesity
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729779/
https://www.ncbi.nlm.nih.gov/pubmed/29069204
http://dx.doi.org/10.5935/abc.20170151
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