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Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics

To investigate the kinetics of Cas9-mediated double strand break generation and repair in vivo, we developed two new tools. The first, chemically inducible Cas9 (ciCas9), is a rapidly-activated, single-component Cas9 variant engineered using a novel domain replacement strategy. ciCas9 can be activat...

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Detalles Bibliográficos
Autores principales:	Rose, John C., Stephany, Jason J., Valente, William J., Trevillian, Bridget M., Dang, Ha V., Bielas, Jason H., Maly, Dustin J., Fowler, Douglas M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730411/ https://www.ncbi.nlm.nih.gov/pubmed/28737741 http://dx.doi.org/10.1038/nmeth.4368

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