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Cell cycle synchronisation of Trypanosoma brucei by centrifugal counter-flow elutriation reveals the timing of nuclear and kinetoplast DNA replication

We report an optimised centrifugal counter-flow elutriation protocol for the rapid and direct isolation of G1 cell cycle synchronised populations of both the procyclic and bloodstream form stages of Trypanosoma brucei that yields viable and proliferative cells. The high quality of the synchronisatio...

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Detalles Bibliográficos
Autores principales:	Benz, Corinna, Dondelinger, Frank, McKean, Paul G., Urbaniak, Michael D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730572/ https://www.ncbi.nlm.nih.gov/pubmed/29242601 http://dx.doi.org/10.1038/s41598-017-17779-z

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