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How zebrafish research has helped in understanding thyroid diseases
Next-generation sequencing technologies have revolutionized the identification of disease-causing genes, accelerating the discovery of new mutations and new candidate genes for thyroid diseases. To face this flow of novel genetic information, it is important to have suitable animal models to study t...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000 Research Limited
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730863/ https://www.ncbi.nlm.nih.gov/pubmed/29263787 http://dx.doi.org/10.12688/f1000research.12142.1 |
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author | Marelli, Federica Persani, Luca |
author_facet | Marelli, Federica Persani, Luca |
author_sort | Marelli, Federica |
collection | PubMed |
description | Next-generation sequencing technologies have revolutionized the identification of disease-causing genes, accelerating the discovery of new mutations and new candidate genes for thyroid diseases. To face this flow of novel genetic information, it is important to have suitable animal models to study the mechanisms regulating thyroid development and thyroid hormone availability and activity. Zebrafish ( Danio rerio), with its rapid external embryonic development, has been extensively used in developmental biology. To date, almost all of the components of the zebrafish thyroid axis have been characterized and are structurally and functionally comparable with those of higher vertebrates. The availability of transgenic fluorescent zebrafish lines allows the real-time analysis of thyroid organogenesis and its alterations. Transient morpholino-knockdown is a solution to silence the expression of a gene of interest and promptly obtain insights on its contribution during the development of the zebrafish thyroid axis. The recently available tools for targeted stable gene knockout have further increased the value of zebrafish to the study of thyroid disease. All of the reported zebrafish models can also be used to screen small compounds and to test new drugs and may allow the establishment of experimental proof of concept to plan subsequent clinical trials. |
format | Online Article Text |
id | pubmed-5730863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-57308632017-12-19 How zebrafish research has helped in understanding thyroid diseases Marelli, Federica Persani, Luca F1000Res Review Next-generation sequencing technologies have revolutionized the identification of disease-causing genes, accelerating the discovery of new mutations and new candidate genes for thyroid diseases. To face this flow of novel genetic information, it is important to have suitable animal models to study the mechanisms regulating thyroid development and thyroid hormone availability and activity. Zebrafish ( Danio rerio), with its rapid external embryonic development, has been extensively used in developmental biology. To date, almost all of the components of the zebrafish thyroid axis have been characterized and are structurally and functionally comparable with those of higher vertebrates. The availability of transgenic fluorescent zebrafish lines allows the real-time analysis of thyroid organogenesis and its alterations. Transient morpholino-knockdown is a solution to silence the expression of a gene of interest and promptly obtain insights on its contribution during the development of the zebrafish thyroid axis. The recently available tools for targeted stable gene knockout have further increased the value of zebrafish to the study of thyroid disease. All of the reported zebrafish models can also be used to screen small compounds and to test new drugs and may allow the establishment of experimental proof of concept to plan subsequent clinical trials. F1000 Research Limited 2017-12-14 /pmc/articles/PMC5730863/ /pubmed/29263787 http://dx.doi.org/10.12688/f1000research.12142.1 Text en Copyright: © 2017 Marelli F and Persani L http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Marelli, Federica Persani, Luca How zebrafish research has helped in understanding thyroid diseases |
title | How zebrafish research has helped in understanding thyroid diseases |
title_full | How zebrafish research has helped in understanding thyroid diseases |
title_fullStr | How zebrafish research has helped in understanding thyroid diseases |
title_full_unstemmed | How zebrafish research has helped in understanding thyroid diseases |
title_short | How zebrafish research has helped in understanding thyroid diseases |
title_sort | how zebrafish research has helped in understanding thyroid diseases |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730863/ https://www.ncbi.nlm.nih.gov/pubmed/29263787 http://dx.doi.org/10.12688/f1000research.12142.1 |
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