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Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology

Particles of cowpea mosaic virus (CPMV) have enjoyed considerable success as nanoparticles. The development of a system for producing empty virus-like particles (eVLPs) of the virus, which are non-infectious and have the potential to be loaded with heterologous material, has increased the number of...

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Autores principales: Meshcheriakova, Yulia, Durrant, Alex, Hesketh, Emma L., Ranson, Neil A., Lomonossoff, George P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730940/
https://www.ncbi.nlm.nih.gov/pubmed/29101307
http://dx.doi.org/10.1042/BST20160312
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author Meshcheriakova, Yulia
Durrant, Alex
Hesketh, Emma L.
Ranson, Neil A.
Lomonossoff, George P.
author_facet Meshcheriakova, Yulia
Durrant, Alex
Hesketh, Emma L.
Ranson, Neil A.
Lomonossoff, George P.
author_sort Meshcheriakova, Yulia
collection PubMed
description Particles of cowpea mosaic virus (CPMV) have enjoyed considerable success as nanoparticles. The development of a system for producing empty virus-like particles (eVLPs) of the virus, which are non-infectious and have the potential to be loaded with heterologous material, has increased the number of possible applications for CPMV-based particles. However, for this potential to be realised, it was essential to demonstrate that eVLPs were accurate surrogates for natural virus particles, and this information was provided by high-resolution cryo-EM studies of eVLPs. This demonstration has enabled the approaches developed for the production of modified particles developed with natural CPMV particles to be applied to eVLPs. Furthermore, a combination of cryo-EM and mutagenic studies allowed the development of particles which are permeable but which could still assemble efficiently. These particles were shown to be loadable with cobalt, indicating that they can, indeed, be used as nano-containers.
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spelling pubmed-57309402017-12-19 Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology Meshcheriakova, Yulia Durrant, Alex Hesketh, Emma L. Ranson, Neil A. Lomonossoff, George P. Biochem Soc Trans Review Articles Particles of cowpea mosaic virus (CPMV) have enjoyed considerable success as nanoparticles. The development of a system for producing empty virus-like particles (eVLPs) of the virus, which are non-infectious and have the potential to be loaded with heterologous material, has increased the number of possible applications for CPMV-based particles. However, for this potential to be realised, it was essential to demonstrate that eVLPs were accurate surrogates for natural virus particles, and this information was provided by high-resolution cryo-EM studies of eVLPs. This demonstration has enabled the approaches developed for the production of modified particles developed with natural CPMV particles to be applied to eVLPs. Furthermore, a combination of cryo-EM and mutagenic studies allowed the development of particles which are permeable but which could still assemble efficiently. These particles were shown to be loadable with cobalt, indicating that they can, indeed, be used as nano-containers. Portland Press Ltd. 2017-12-15 2017-11-03 /pmc/articles/PMC5730940/ /pubmed/29101307 http://dx.doi.org/10.1042/BST20160312 Text en © 2017 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review Articles
Meshcheriakova, Yulia
Durrant, Alex
Hesketh, Emma L.
Ranson, Neil A.
Lomonossoff, George P.
Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology
title Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology
title_full Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology
title_fullStr Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology
title_full_unstemmed Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology
title_short Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology
title_sort combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730940/
https://www.ncbi.nlm.nih.gov/pubmed/29101307
http://dx.doi.org/10.1042/BST20160312
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