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Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly

Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to eval...

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Autores principales: Mendes, G.A., Haag, T., Trott, G., Rech, C.G.S.L., Ferreira, N.P., Oliveira, M.C., Kohek, M.B., Pereira-Lima, J.F.S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731331/
https://www.ncbi.nlm.nih.gov/pubmed/29267504
http://dx.doi.org/10.1590/1414-431X20176808
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author Mendes, G.A.
Haag, T.
Trott, G.
Rech, C.G.S.L.
Ferreira, N.P.
Oliveira, M.C.
Kohek, M.B.
Pereira-Lima, J.F.S.
author_facet Mendes, G.A.
Haag, T.
Trott, G.
Rech, C.G.S.L.
Ferreira, N.P.
Oliveira, M.C.
Kohek, M.B.
Pereira-Lima, J.F.S.
author_sort Mendes, G.A.
collection PubMed
description Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to evaluate E-cadherin, Slug and neural cell adhesion molecule (NCAM) expression in GH-secreting pituitary adenomas and its relationship to tumor invasiveness. A cross–sectional study of patients who underwent hypophysectomy due to GH-secreting pituitary adenoma from April 2007 to December 2014 was carried out. The medical records were reviewed to collect clinical data. Immediately after surgery, tumor samples were frozen in liquid nitrogen and stored in a biofreezer at –80°C for assessment of E-cadherin 1 (CDH1), SLUG (SNAI2), and NCAM (NCAM1) by real-time PCR. The samples were fixed in formalin and embedded in paraffin for immunohistochemical analysis of E-cadherin and NCAM. Thirty-five patients with acromegaly were included in the study. Of these, 65.7% had invasive tumors. Immunohistochemically, E-cadherin was expressed in 96.7% of patients, and NCAM in 80% of patients. There was no statistically significant relationship between tumor grade or invasiveness and immunohistochemical expression of these markers. Regarding gene expression, 50% of cases expressed CDH1, none expressed SNAI2, and 53.3% expressed NCAM1. There was no statistically significant relationship between tumor grade or invasiveness and gene expression of CDH1, SNAI2, and NCAM1. The absence of Slug overexpression and of E-cadherin and NCAM suppression suggests that expression of these markers is not associated with tumor invasiveness in GH-secreting pituitary adenomas.
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spelling pubmed-57313312017-12-28 Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly Mendes, G.A. Haag, T. Trott, G. Rech, C.G.S.L. Ferreira, N.P. Oliveira, M.C. Kohek, M.B. Pereira-Lima, J.F.S. Braz J Med Biol Res Research Articles Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to evaluate E-cadherin, Slug and neural cell adhesion molecule (NCAM) expression in GH-secreting pituitary adenomas and its relationship to tumor invasiveness. A cross–sectional study of patients who underwent hypophysectomy due to GH-secreting pituitary adenoma from April 2007 to December 2014 was carried out. The medical records were reviewed to collect clinical data. Immediately after surgery, tumor samples were frozen in liquid nitrogen and stored in a biofreezer at –80°C for assessment of E-cadherin 1 (CDH1), SLUG (SNAI2), and NCAM (NCAM1) by real-time PCR. The samples were fixed in formalin and embedded in paraffin for immunohistochemical analysis of E-cadherin and NCAM. Thirty-five patients with acromegaly were included in the study. Of these, 65.7% had invasive tumors. Immunohistochemically, E-cadherin was expressed in 96.7% of patients, and NCAM in 80% of patients. There was no statistically significant relationship between tumor grade or invasiveness and immunohistochemical expression of these markers. Regarding gene expression, 50% of cases expressed CDH1, none expressed SNAI2, and 53.3% expressed NCAM1. There was no statistically significant relationship between tumor grade or invasiveness and gene expression of CDH1, SNAI2, and NCAM1. The absence of Slug overexpression and of E-cadherin and NCAM suppression suggests that expression of these markers is not associated with tumor invasiveness in GH-secreting pituitary adenomas. Associação Brasileira de Divulgação Científica 2017-12-11 /pmc/articles/PMC5731331/ /pubmed/29267504 http://dx.doi.org/10.1590/1414-431X20176808 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Mendes, G.A.
Haag, T.
Trott, G.
Rech, C.G.S.L.
Ferreira, N.P.
Oliveira, M.C.
Kohek, M.B.
Pereira-Lima, J.F.S.
Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly
title Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly
title_full Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly
title_fullStr Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly
title_full_unstemmed Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly
title_short Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly
title_sort expression of e-cadherin, slug and ncam and its relationship to tumor invasiveness in patients with acromegaly
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731331/
https://www.ncbi.nlm.nih.gov/pubmed/29267504
http://dx.doi.org/10.1590/1414-431X20176808
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