Plasma Cytochrome c Detection Using a Highly Sensitive Electrochemiluminescence Enzyme-Linked Immunosorbent Assay

BACKGROUND: Cytochrome c is an intermembrane mitochondrial protein that is released to the bloodstream following mitochondrial injury. METHODS AND RESULTS: We developed an electrochemiluminescence immunoassay to measure cytochrome c in human and rat plasma, which showed high sensitivity with broad d...

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Detalles Bibliográficos
Autores principales: Radhakrishnan, Jeejabai, Origenes, Rovi, Littlejohn, Gina, Nikolich, Sanja, Choi, Eunjung, Smite, Sharon, Lamoureux, Lorissa, Baetiong, Alvin, Shah, Manoj, Gazmuri, Raúl J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2017
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731609/
https://www.ncbi.nlm.nih.gov/pubmed/29276374
http://dx.doi.org/10.1177/1177271917746972
Descripción
Sumario:BACKGROUND: Cytochrome c is an intermembrane mitochondrial protein that is released to the bloodstream following mitochondrial injury. METHODS AND RESULTS: We developed an electrochemiluminescence immunoassay to measure cytochrome c in human and rat plasma, which showed high sensitivity with broad dynamic range (2-1200 ng/mL in humans and 5-500 ng/mL in rat) and high assay reproducibility (inter-assay coefficient <6% in humans and <10% in rat). In patients after blunt trauma, plasma cytochrome c directly correlated with injury severity. In rats after cardiac resuscitation, plasma cytochrome c inversely correlated with survival and responsiveness to mitochondrial protective interventions. CONCLUSIONS: The cytochrome c assays herein presented have high sensitivity, wide dynamic range, and high reproducibility well suited for biomarker of mitochondrial injury.

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