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Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei
CLC type anion transport proteins are homo-dimeric or hetero-dimeric with an integrated transport function in each subunit. We have identified and partially characterized three members of this family named TbVCL1, TbVCL2 and TbVCL3 in Trypanosoma brucei. Among the human CLC family members, the T. br...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731698/ https://www.ncbi.nlm.nih.gov/pubmed/29244877 http://dx.doi.org/10.1371/journal.pone.0188219 |
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author | Steinmann, Michael E. Schmidt, Remo S. Macêdo, Juan P. Kunz Renggli, Christina Bütikofer, Peter Rentsch, Doris Mäser, Pascal Sigel, Erwin |
author_facet | Steinmann, Michael E. Schmidt, Remo S. Macêdo, Juan P. Kunz Renggli, Christina Bütikofer, Peter Rentsch, Doris Mäser, Pascal Sigel, Erwin |
author_sort | Steinmann, Michael E. |
collection | PubMed |
description | CLC type anion transport proteins are homo-dimeric or hetero-dimeric with an integrated transport function in each subunit. We have identified and partially characterized three members of this family named TbVCL1, TbVCL2 and TbVCL3 in Trypanosoma brucei. Among the human CLC family members, the T. brucei proteins display highest similarity to CLC-6 and CLC-7. TbVCL1, but not TbVCL2 and TbVCL3 is able to complement growth of a CLC-deficient Saccharomyces cerevisiae mutant. All TbVCL-HA fusion proteins localize intracellulary in procyclic form trypanosomes. TbVCL1 localizes close to the Golgi apparatus and TbVCL2 and TbVCL3 to the endoplasmic reticulum. Upon expression in Xenopus oocytes, all three proteins induce similar outward rectifying chloride ion currents. Currents are sensitive to low concentrations of DIDS, insensitive to the pH in the range 5.4 to 8.4 and larger in nitrate than in chloride medium. |
format | Online Article Text |
id | pubmed-5731698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57316982017-12-22 Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei Steinmann, Michael E. Schmidt, Remo S. Macêdo, Juan P. Kunz Renggli, Christina Bütikofer, Peter Rentsch, Doris Mäser, Pascal Sigel, Erwin PLoS One Research Article CLC type anion transport proteins are homo-dimeric or hetero-dimeric with an integrated transport function in each subunit. We have identified and partially characterized three members of this family named TbVCL1, TbVCL2 and TbVCL3 in Trypanosoma brucei. Among the human CLC family members, the T. brucei proteins display highest similarity to CLC-6 and CLC-7. TbVCL1, but not TbVCL2 and TbVCL3 is able to complement growth of a CLC-deficient Saccharomyces cerevisiae mutant. All TbVCL-HA fusion proteins localize intracellulary in procyclic form trypanosomes. TbVCL1 localizes close to the Golgi apparatus and TbVCL2 and TbVCL3 to the endoplasmic reticulum. Upon expression in Xenopus oocytes, all three proteins induce similar outward rectifying chloride ion currents. Currents are sensitive to low concentrations of DIDS, insensitive to the pH in the range 5.4 to 8.4 and larger in nitrate than in chloride medium. Public Library of Science 2017-12-15 /pmc/articles/PMC5731698/ /pubmed/29244877 http://dx.doi.org/10.1371/journal.pone.0188219 Text en © 2017 Steinmann et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Steinmann, Michael E. Schmidt, Remo S. Macêdo, Juan P. Kunz Renggli, Christina Bütikofer, Peter Rentsch, Doris Mäser, Pascal Sigel, Erwin Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei |
title | Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei |
title_full | Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei |
title_fullStr | Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei |
title_full_unstemmed | Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei |
title_short | Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei |
title_sort | identification and characterization of the three members of the clc family of anion transport proteins in trypanosoma brucei |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731698/ https://www.ncbi.nlm.nih.gov/pubmed/29244877 http://dx.doi.org/10.1371/journal.pone.0188219 |
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