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Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells
Curcumin has protective effects against toxic agents and shows preventive properties for various diseases. Particulate material with an aerodynamic diameter of ≤10 μm (PM(10)) and titanium dioxide nanoparticles (TiO(2)-NPs) induce endothelial dysfunction and activation. We explored whether curcumin...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731739/ https://www.ncbi.nlm.nih.gov/pubmed/29244817 http://dx.doi.org/10.1371/journal.pone.0188169 |
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author | Montiel-Dávalos, Angélica Silva Sánchez, Guadalupe Jazmin Huerta-García, Elizabeth Rueda-Romero, Cristhiam Soca Chafre, Giovanny Mitre-Aguilar, Irma B. Alfaro-Moreno, Ernesto Pedraza-Chaverri, José López-Marure, Rebeca |
author_facet | Montiel-Dávalos, Angélica Silva Sánchez, Guadalupe Jazmin Huerta-García, Elizabeth Rueda-Romero, Cristhiam Soca Chafre, Giovanny Mitre-Aguilar, Irma B. Alfaro-Moreno, Ernesto Pedraza-Chaverri, José López-Marure, Rebeca |
author_sort | Montiel-Dávalos, Angélica |
collection | PubMed |
description | Curcumin has protective effects against toxic agents and shows preventive properties for various diseases. Particulate material with an aerodynamic diameter of ≤10 μm (PM(10)) and titanium dioxide nanoparticles (TiO(2)-NPs) induce endothelial dysfunction and activation. We explored whether curcumin is able to attenuate different events related to endothelial activation. This includes adhesion, expression of adhesion molecules and oxidative stress induced by PM(10) and TiO(2)-NPs. Human umbilical vein endothelial cells (HUVEC) were treated with 1, 10 and 100 μM curcumin for 1 h and then exposed to PM(10) at 3 μg/cm(2) or TiO(2)-NPs at 10 μg/cm(2). Cell adhesion was evaluated by co-culture with U937 human myelomonocytic cells. Adhesion molecules expression was measured by flow cytometry after 3 or 24 h of exposure. Oxidative stress was determined by 2,7-dichlorodihydrofluorescein (H(2)DCF) oxidation. PM(10) and TiO(2)-NPs induced the adhesion of U937 cells and the expression of E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and platelet-endothelial cell adhesion molecule-1 (PECAM-1). The expression of E- and P-selectins matched the adhesion of monocytes to HUVEC after 3 h. In HUVEC treated with 1 or 10 μM curcumin, the expression of adhesion molecules and monocytes adhesion was significantly diminished. Curcumin also partially reduced the H(2)DCF oxidation induced by PM(10) and TiO(2)-NPs. Our results suggest an anti-inflammatory and antioxidant role by curcumin attenuating the activation caused on endothelial cells by exposure to particles. Therefore, curcumin could be useful in the treatment of diseases where an inflammatory process and endothelial activation are involved. |
format | Online Article Text |
id | pubmed-5731739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57317392017-12-22 Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells Montiel-Dávalos, Angélica Silva Sánchez, Guadalupe Jazmin Huerta-García, Elizabeth Rueda-Romero, Cristhiam Soca Chafre, Giovanny Mitre-Aguilar, Irma B. Alfaro-Moreno, Ernesto Pedraza-Chaverri, José López-Marure, Rebeca PLoS One Research Article Curcumin has protective effects against toxic agents and shows preventive properties for various diseases. Particulate material with an aerodynamic diameter of ≤10 μm (PM(10)) and titanium dioxide nanoparticles (TiO(2)-NPs) induce endothelial dysfunction and activation. We explored whether curcumin is able to attenuate different events related to endothelial activation. This includes adhesion, expression of adhesion molecules and oxidative stress induced by PM(10) and TiO(2)-NPs. Human umbilical vein endothelial cells (HUVEC) were treated with 1, 10 and 100 μM curcumin for 1 h and then exposed to PM(10) at 3 μg/cm(2) or TiO(2)-NPs at 10 μg/cm(2). Cell adhesion was evaluated by co-culture with U937 human myelomonocytic cells. Adhesion molecules expression was measured by flow cytometry after 3 or 24 h of exposure. Oxidative stress was determined by 2,7-dichlorodihydrofluorescein (H(2)DCF) oxidation. PM(10) and TiO(2)-NPs induced the adhesion of U937 cells and the expression of E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and platelet-endothelial cell adhesion molecule-1 (PECAM-1). The expression of E- and P-selectins matched the adhesion of monocytes to HUVEC after 3 h. In HUVEC treated with 1 or 10 μM curcumin, the expression of adhesion molecules and monocytes adhesion was significantly diminished. Curcumin also partially reduced the H(2)DCF oxidation induced by PM(10) and TiO(2)-NPs. Our results suggest an anti-inflammatory and antioxidant role by curcumin attenuating the activation caused on endothelial cells by exposure to particles. Therefore, curcumin could be useful in the treatment of diseases where an inflammatory process and endothelial activation are involved. Public Library of Science 2017-12-15 /pmc/articles/PMC5731739/ /pubmed/29244817 http://dx.doi.org/10.1371/journal.pone.0188169 Text en © 2017 Montiel-Dávalos et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Montiel-Dávalos, Angélica Silva Sánchez, Guadalupe Jazmin Huerta-García, Elizabeth Rueda-Romero, Cristhiam Soca Chafre, Giovanny Mitre-Aguilar, Irma B. Alfaro-Moreno, Ernesto Pedraza-Chaverri, José López-Marure, Rebeca Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells |
title | Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells |
title_full | Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells |
title_fullStr | Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells |
title_full_unstemmed | Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells |
title_short | Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells |
title_sort | curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731739/ https://www.ncbi.nlm.nih.gov/pubmed/29244817 http://dx.doi.org/10.1371/journal.pone.0188169 |
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