Cargando…

Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells

Curcumin has protective effects against toxic agents and shows preventive properties for various diseases. Particulate material with an aerodynamic diameter of ≤10 μm (PM(10)) and titanium dioxide nanoparticles (TiO(2)-NPs) induce endothelial dysfunction and activation. We explored whether curcumin...

Descripción completa

Detalles Bibliográficos
Autores principales: Montiel-Dávalos, Angélica, Silva Sánchez, Guadalupe Jazmin, Huerta-García, Elizabeth, Rueda-Romero, Cristhiam, Soca Chafre, Giovanny, Mitre-Aguilar, Irma B., Alfaro-Moreno, Ernesto, Pedraza-Chaverri, José, López-Marure, Rebeca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731739/
https://www.ncbi.nlm.nih.gov/pubmed/29244817
http://dx.doi.org/10.1371/journal.pone.0188169
_version_ 1783286559295930368
author Montiel-Dávalos, Angélica
Silva Sánchez, Guadalupe Jazmin
Huerta-García, Elizabeth
Rueda-Romero, Cristhiam
Soca Chafre, Giovanny
Mitre-Aguilar, Irma B.
Alfaro-Moreno, Ernesto
Pedraza-Chaverri, José
López-Marure, Rebeca
author_facet Montiel-Dávalos, Angélica
Silva Sánchez, Guadalupe Jazmin
Huerta-García, Elizabeth
Rueda-Romero, Cristhiam
Soca Chafre, Giovanny
Mitre-Aguilar, Irma B.
Alfaro-Moreno, Ernesto
Pedraza-Chaverri, José
López-Marure, Rebeca
author_sort Montiel-Dávalos, Angélica
collection PubMed
description Curcumin has protective effects against toxic agents and shows preventive properties for various diseases. Particulate material with an aerodynamic diameter of ≤10 μm (PM(10)) and titanium dioxide nanoparticles (TiO(2)-NPs) induce endothelial dysfunction and activation. We explored whether curcumin is able to attenuate different events related to endothelial activation. This includes adhesion, expression of adhesion molecules and oxidative stress induced by PM(10) and TiO(2)-NPs. Human umbilical vein endothelial cells (HUVEC) were treated with 1, 10 and 100 μM curcumin for 1 h and then exposed to PM(10) at 3 μg/cm(2) or TiO(2)-NPs at 10 μg/cm(2). Cell adhesion was evaluated by co-culture with U937 human myelomonocytic cells. Adhesion molecules expression was measured by flow cytometry after 3 or 24 h of exposure. Oxidative stress was determined by 2,7-dichlorodihydrofluorescein (H(2)DCF) oxidation. PM(10) and TiO(2)-NPs induced the adhesion of U937 cells and the expression of E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and platelet-endothelial cell adhesion molecule-1 (PECAM-1). The expression of E- and P-selectins matched the adhesion of monocytes to HUVEC after 3 h. In HUVEC treated with 1 or 10 μM curcumin, the expression of adhesion molecules and monocytes adhesion was significantly diminished. Curcumin also partially reduced the H(2)DCF oxidation induced by PM(10) and TiO(2)-NPs. Our results suggest an anti-inflammatory and antioxidant role by curcumin attenuating the activation caused on endothelial cells by exposure to particles. Therefore, curcumin could be useful in the treatment of diseases where an inflammatory process and endothelial activation are involved.
format Online
Article
Text
id pubmed-5731739
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-57317392017-12-22 Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells Montiel-Dávalos, Angélica Silva Sánchez, Guadalupe Jazmin Huerta-García, Elizabeth Rueda-Romero, Cristhiam Soca Chafre, Giovanny Mitre-Aguilar, Irma B. Alfaro-Moreno, Ernesto Pedraza-Chaverri, José López-Marure, Rebeca PLoS One Research Article Curcumin has protective effects against toxic agents and shows preventive properties for various diseases. Particulate material with an aerodynamic diameter of ≤10 μm (PM(10)) and titanium dioxide nanoparticles (TiO(2)-NPs) induce endothelial dysfunction and activation. We explored whether curcumin is able to attenuate different events related to endothelial activation. This includes adhesion, expression of adhesion molecules and oxidative stress induced by PM(10) and TiO(2)-NPs. Human umbilical vein endothelial cells (HUVEC) were treated with 1, 10 and 100 μM curcumin for 1 h and then exposed to PM(10) at 3 μg/cm(2) or TiO(2)-NPs at 10 μg/cm(2). Cell adhesion was evaluated by co-culture with U937 human myelomonocytic cells. Adhesion molecules expression was measured by flow cytometry after 3 or 24 h of exposure. Oxidative stress was determined by 2,7-dichlorodihydrofluorescein (H(2)DCF) oxidation. PM(10) and TiO(2)-NPs induced the adhesion of U937 cells and the expression of E- and P-selectins, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and platelet-endothelial cell adhesion molecule-1 (PECAM-1). The expression of E- and P-selectins matched the adhesion of monocytes to HUVEC after 3 h. In HUVEC treated with 1 or 10 μM curcumin, the expression of adhesion molecules and monocytes adhesion was significantly diminished. Curcumin also partially reduced the H(2)DCF oxidation induced by PM(10) and TiO(2)-NPs. Our results suggest an anti-inflammatory and antioxidant role by curcumin attenuating the activation caused on endothelial cells by exposure to particles. Therefore, curcumin could be useful in the treatment of diseases where an inflammatory process and endothelial activation are involved. Public Library of Science 2017-12-15 /pmc/articles/PMC5731739/ /pubmed/29244817 http://dx.doi.org/10.1371/journal.pone.0188169 Text en © 2017 Montiel-Dávalos et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Montiel-Dávalos, Angélica
Silva Sánchez, Guadalupe Jazmin
Huerta-García, Elizabeth
Rueda-Romero, Cristhiam
Soca Chafre, Giovanny
Mitre-Aguilar, Irma B.
Alfaro-Moreno, Ernesto
Pedraza-Chaverri, José
López-Marure, Rebeca
Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells
title Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells
title_full Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells
title_fullStr Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells
title_full_unstemmed Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells
title_short Curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells
title_sort curcumin inhibits activation induced by urban particulate material or titanium dioxide nanoparticles in primary human endothelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731739/
https://www.ncbi.nlm.nih.gov/pubmed/29244817
http://dx.doi.org/10.1371/journal.pone.0188169
work_keys_str_mv AT montieldavalosangelica curcumininhibitsactivationinducedbyurbanparticulatematerialortitaniumdioxidenanoparticlesinprimaryhumanendothelialcells
AT silvasanchezguadalupejazmin curcumininhibitsactivationinducedbyurbanparticulatematerialortitaniumdioxidenanoparticlesinprimaryhumanendothelialcells
AT huertagarciaelizabeth curcumininhibitsactivationinducedbyurbanparticulatematerialortitaniumdioxidenanoparticlesinprimaryhumanendothelialcells
AT ruedaromerocristhiam curcumininhibitsactivationinducedbyurbanparticulatematerialortitaniumdioxidenanoparticlesinprimaryhumanendothelialcells
AT socachafregiovanny curcumininhibitsactivationinducedbyurbanparticulatematerialortitaniumdioxidenanoparticlesinprimaryhumanendothelialcells
AT mitreaguilarirmab curcumininhibitsactivationinducedbyurbanparticulatematerialortitaniumdioxidenanoparticlesinprimaryhumanendothelialcells
AT alfaromorenoernesto curcumininhibitsactivationinducedbyurbanparticulatematerialortitaniumdioxidenanoparticlesinprimaryhumanendothelialcells
AT pedrazachaverrijose curcumininhibitsactivationinducedbyurbanparticulatematerialortitaniumdioxidenanoparticlesinprimaryhumanendothelialcells
AT lopezmarurerebeca curcumininhibitsactivationinducedbyurbanparticulatematerialortitaniumdioxidenanoparticlesinprimaryhumanendothelialcells