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Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis

During vertebrate heart development, two progenitor populations, first and second heart fields (FHF, SHF), sequentially contribute to longitudinal subdivisions of the heart tube (HT), with the FHF contributing the left ventricle and part of the atria, and the SHF the rest of the heart. Here, we stud...

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Autores principales: Ivanovitch, Kenzo, Temiño, Susana, Torres, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731822/
https://www.ncbi.nlm.nih.gov/pubmed/29202929
http://dx.doi.org/10.7554/eLife.30668
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author Ivanovitch, Kenzo
Temiño, Susana
Torres, Miguel
author_facet Ivanovitch, Kenzo
Temiño, Susana
Torres, Miguel
author_sort Ivanovitch, Kenzo
collection PubMed
description During vertebrate heart development, two progenitor populations, first and second heart fields (FHF, SHF), sequentially contribute to longitudinal subdivisions of the heart tube (HT), with the FHF contributing the left ventricle and part of the atria, and the SHF the rest of the heart. Here, we study the dynamics of cardiac differentiation and morphogenesis by tracking individual cells in live analysis of mouse embryos. We report that during an initial phase, FHF precursors differentiate rapidly to form a cardiac crescent, while limited morphogenesis takes place. In a second phase, no differentiation occurs while extensive morphogenesis, including splanchnic mesoderm sliding over the endoderm, results in HT formation. In a third phase, cardiac precursor differentiation resumes and contributes to SHF-derived regions and the dorsal closure of the HT. These results reveal tissue-level coordination between morphogenesis and differentiation during HT formation and provide a new framework to understand heart development.
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spelling pubmed-57318222017-12-15 Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis Ivanovitch, Kenzo Temiño, Susana Torres, Miguel eLife Developmental Biology During vertebrate heart development, two progenitor populations, first and second heart fields (FHF, SHF), sequentially contribute to longitudinal subdivisions of the heart tube (HT), with the FHF contributing the left ventricle and part of the atria, and the SHF the rest of the heart. Here, we study the dynamics of cardiac differentiation and morphogenesis by tracking individual cells in live analysis of mouse embryos. We report that during an initial phase, FHF precursors differentiate rapidly to form a cardiac crescent, while limited morphogenesis takes place. In a second phase, no differentiation occurs while extensive morphogenesis, including splanchnic mesoderm sliding over the endoderm, results in HT formation. In a third phase, cardiac precursor differentiation resumes and contributes to SHF-derived regions and the dorsal closure of the HT. These results reveal tissue-level coordination between morphogenesis and differentiation during HT formation and provide a new framework to understand heart development. eLife Sciences Publications, Ltd 2017-12-05 /pmc/articles/PMC5731822/ /pubmed/29202929 http://dx.doi.org/10.7554/eLife.30668 Text en © 2017, Ivanovitch et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Ivanovitch, Kenzo
Temiño, Susana
Torres, Miguel
Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis
title Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis
title_full Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis
title_fullStr Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis
title_full_unstemmed Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis
title_short Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis
title_sort live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731822/
https://www.ncbi.nlm.nih.gov/pubmed/29202929
http://dx.doi.org/10.7554/eLife.30668
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