MicroRNA-363 inhibits ovarian cancer progression by inhibiting NOB1

In this study, we investigated the role of microRNA-363(miR-363) in ovarian cancer (OC) progression. MiR-363expression was downregulated in OC patient tissues and four OC cell lines (SKOV3, A2780, OVCAR and HO-8910). Low miR-363 levels were associated with advanced stage, lymph node metastasis, and...

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Detalles Bibliográficos
Autores principales: Lin, Yang, Xu, Tianmin, Zhou, Shunqing, Cui, Manhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731903/
https://www.ncbi.nlm.nih.gov/pubmed/29254193
http://dx.doi.org/10.18632/oncotarget.21417
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author Lin, Yang
Xu, Tianmin
Zhou, Shunqing
Cui, Manhua
author_facet Lin, Yang
Xu, Tianmin
Zhou, Shunqing
Cui, Manhua
author_sort Lin, Yang
collection PubMed
description In this study, we investigated the role of microRNA-363(miR-363) in ovarian cancer (OC) progression. MiR-363expression was downregulated in OC patient tissues and four OC cell lines (SKOV3, A2780, OVCAR and HO-8910). Low miR-363 levels were associated with advanced stage, lymph node metastasis, and poor prognosis in OC. MiR-363 overexpression decreased growth, colony formation, migration and invasiveness of SKOV3 cells. In addition, miR-363 overexpression in SKOV3 cells also decreased xenograft tumor size and weight in nude mice. Bioinformatics and dual luciferase reporter assays revealed that miR-363 suppresses expression of NIN1/RPN12 binding protein 1 homolog (NOB1) by binding to the 3’-UTR of its transcript. NOB1 expression inversely correlated with miR-363 levels in OC tissues. Thus miR-363 appears to play a tumor suppressor role in OC by inhibiting NOB1.
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spelling pubmed-57319032017-12-17 MicroRNA-363 inhibits ovarian cancer progression by inhibiting NOB1 Lin, Yang Xu, Tianmin Zhou, Shunqing Cui, Manhua Oncotarget Research Paper In this study, we investigated the role of microRNA-363(miR-363) in ovarian cancer (OC) progression. MiR-363expression was downregulated in OC patient tissues and four OC cell lines (SKOV3, A2780, OVCAR and HO-8910). Low miR-363 levels were associated with advanced stage, lymph node metastasis, and poor prognosis in OC. MiR-363 overexpression decreased growth, colony formation, migration and invasiveness of SKOV3 cells. In addition, miR-363 overexpression in SKOV3 cells also decreased xenograft tumor size and weight in nude mice. Bioinformatics and dual luciferase reporter assays revealed that miR-363 suppresses expression of NIN1/RPN12 binding protein 1 homolog (NOB1) by binding to the 3’-UTR of its transcript. NOB1 expression inversely correlated with miR-363 levels in OC tissues. Thus miR-363 appears to play a tumor suppressor role in OC by inhibiting NOB1. Impact Journals LLC 2017-09-30 /pmc/articles/PMC5731903/ /pubmed/29254193 http://dx.doi.org/10.18632/oncotarget.21417 Text en Copyright: © 2017 Lin et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Lin, Yang
Xu, Tianmin
Zhou, Shunqing
Cui, Manhua
MicroRNA-363 inhibits ovarian cancer progression by inhibiting NOB1
title MicroRNA-363 inhibits ovarian cancer progression by inhibiting NOB1
title_full MicroRNA-363 inhibits ovarian cancer progression by inhibiting NOB1
title_fullStr MicroRNA-363 inhibits ovarian cancer progression by inhibiting NOB1
title_full_unstemmed MicroRNA-363 inhibits ovarian cancer progression by inhibiting NOB1
title_short MicroRNA-363 inhibits ovarian cancer progression by inhibiting NOB1
title_sort microrna-363 inhibits ovarian cancer progression by inhibiting nob1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731903/
https://www.ncbi.nlm.nih.gov/pubmed/29254193
http://dx.doi.org/10.18632/oncotarget.21417
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