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Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy

The objective of this study was to investigate alterations in human first-trimester decidual immune cells (DICs) and relevant cytokines after treatment with prednisone. Decidual lymphocytes were treated with prednisone alone, cytokines alone or the combination of prednisone and cytokines. Levels of...

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Autores principales: Fu, Xiao-Qian, Cai, Jun-Ying, Huang, Qian-Yi, Li, Dong-Ju, Li, Ning, Li, Mu-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731945/
https://www.ncbi.nlm.nih.gov/pubmed/29254235
http://dx.doi.org/10.18632/oncotarget.22188
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author Fu, Xiao-Qian
Cai, Jun-Ying
Huang, Qian-Yi
Li, Dong-Ju
Li, Ning
Li, Mu-Jun
author_facet Fu, Xiao-Qian
Cai, Jun-Ying
Huang, Qian-Yi
Li, Dong-Ju
Li, Ning
Li, Mu-Jun
author_sort Fu, Xiao-Qian
collection PubMed
description The objective of this study was to investigate alterations in human first-trimester decidual immune cells (DICs) and relevant cytokines after treatment with prednisone. Decidual lymphocytes were treated with prednisone alone, cytokines alone or the combination of prednisone and cytokines. Levels of STAT3, STAT5, RORC and FOXP3 mRNA were assayed using quantitative real-time PCR, proportions of CD4(+) T helper 17 (Th17) and CD4(+) T regulatory (Treg) cells were measured using flow cytometry, and concentrations of interleukin (IL)-17A and IL-10 were determined using enzyme-linked immunosorbent assay. After treatment with prednisone alone, levels of STAT5 and FOXP3 mRNA were significantly higher than in untreated control cells (both P < 0.01), while levels of RORC mRNA were significantly lower than in controls (P < 0.05). Levels of STAT3 mRNA did not vary significantly with treatment. After treatment with prednisone alone, proportions of Th17/CD4(+) cells and levels of IL-17A were significantly lower than in control cells, and proportions of Treg/CD4(+) cells and levels of IL-10 significantly higher than in controls (all P < 0.01). Our results suggest that prednisone may improve pregnancy outcomes by restoring immunological homeostasis through up-regulation of STAT5 and FOXP3, induction of DIC differentiation into Treg cells, inhibition of DIC differentiation into Th17 cells, reduction of IL-17A secretion and induction of IL-10 secretion.
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spelling pubmed-57319452017-12-17 Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy Fu, Xiao-Qian Cai, Jun-Ying Huang, Qian-Yi Li, Dong-Ju Li, Ning Li, Mu-Jun Oncotarget Research Paper The objective of this study was to investigate alterations in human first-trimester decidual immune cells (DICs) and relevant cytokines after treatment with prednisone. Decidual lymphocytes were treated with prednisone alone, cytokines alone or the combination of prednisone and cytokines. Levels of STAT3, STAT5, RORC and FOXP3 mRNA were assayed using quantitative real-time PCR, proportions of CD4(+) T helper 17 (Th17) and CD4(+) T regulatory (Treg) cells were measured using flow cytometry, and concentrations of interleukin (IL)-17A and IL-10 were determined using enzyme-linked immunosorbent assay. After treatment with prednisone alone, levels of STAT5 and FOXP3 mRNA were significantly higher than in untreated control cells (both P < 0.01), while levels of RORC mRNA were significantly lower than in controls (P < 0.05). Levels of STAT3 mRNA did not vary significantly with treatment. After treatment with prednisone alone, proportions of Th17/CD4(+) cells and levels of IL-17A were significantly lower than in control cells, and proportions of Treg/CD4(+) cells and levels of IL-10 significantly higher than in controls (all P < 0.01). Our results suggest that prednisone may improve pregnancy outcomes by restoring immunological homeostasis through up-regulation of STAT5 and FOXP3, induction of DIC differentiation into Treg cells, inhibition of DIC differentiation into Th17 cells, reduction of IL-17A secretion and induction of IL-10 secretion. Impact Journals LLC 2017-10-31 /pmc/articles/PMC5731945/ /pubmed/29254235 http://dx.doi.org/10.18632/oncotarget.22188 Text en Copyright: © 2017 Fu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Fu, Xiao-Qian
Cai, Jun-Ying
Huang, Qian-Yi
Li, Dong-Ju
Li, Ning
Li, Mu-Jun
Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy
title Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy
title_full Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy
title_fullStr Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy
title_full_unstemmed Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy
title_short Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy
title_sort prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731945/
https://www.ncbi.nlm.nih.gov/pubmed/29254235
http://dx.doi.org/10.18632/oncotarget.22188
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