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Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy
The objective of this study was to investigate alterations in human first-trimester decidual immune cells (DICs) and relevant cytokines after treatment with prednisone. Decidual lymphocytes were treated with prednisone alone, cytokines alone or the combination of prednisone and cytokines. Levels of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731945/ https://www.ncbi.nlm.nih.gov/pubmed/29254235 http://dx.doi.org/10.18632/oncotarget.22188 |
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author | Fu, Xiao-Qian Cai, Jun-Ying Huang, Qian-Yi Li, Dong-Ju Li, Ning Li, Mu-Jun |
author_facet | Fu, Xiao-Qian Cai, Jun-Ying Huang, Qian-Yi Li, Dong-Ju Li, Ning Li, Mu-Jun |
author_sort | Fu, Xiao-Qian |
collection | PubMed |
description | The objective of this study was to investigate alterations in human first-trimester decidual immune cells (DICs) and relevant cytokines after treatment with prednisone. Decidual lymphocytes were treated with prednisone alone, cytokines alone or the combination of prednisone and cytokines. Levels of STAT3, STAT5, RORC and FOXP3 mRNA were assayed using quantitative real-time PCR, proportions of CD4(+) T helper 17 (Th17) and CD4(+) T regulatory (Treg) cells were measured using flow cytometry, and concentrations of interleukin (IL)-17A and IL-10 were determined using enzyme-linked immunosorbent assay. After treatment with prednisone alone, levels of STAT5 and FOXP3 mRNA were significantly higher than in untreated control cells (both P < 0.01), while levels of RORC mRNA were significantly lower than in controls (P < 0.05). Levels of STAT3 mRNA did not vary significantly with treatment. After treatment with prednisone alone, proportions of Th17/CD4(+) cells and levels of IL-17A were significantly lower than in control cells, and proportions of Treg/CD4(+) cells and levels of IL-10 significantly higher than in controls (all P < 0.01). Our results suggest that prednisone may improve pregnancy outcomes by restoring immunological homeostasis through up-regulation of STAT5 and FOXP3, induction of DIC differentiation into Treg cells, inhibition of DIC differentiation into Th17 cells, reduction of IL-17A secretion and induction of IL-10 secretion. |
format | Online Article Text |
id | pubmed-5731945 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57319452017-12-17 Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy Fu, Xiao-Qian Cai, Jun-Ying Huang, Qian-Yi Li, Dong-Ju Li, Ning Li, Mu-Jun Oncotarget Research Paper The objective of this study was to investigate alterations in human first-trimester decidual immune cells (DICs) and relevant cytokines after treatment with prednisone. Decidual lymphocytes were treated with prednisone alone, cytokines alone or the combination of prednisone and cytokines. Levels of STAT3, STAT5, RORC and FOXP3 mRNA were assayed using quantitative real-time PCR, proportions of CD4(+) T helper 17 (Th17) and CD4(+) T regulatory (Treg) cells were measured using flow cytometry, and concentrations of interleukin (IL)-17A and IL-10 were determined using enzyme-linked immunosorbent assay. After treatment with prednisone alone, levels of STAT5 and FOXP3 mRNA were significantly higher than in untreated control cells (both P < 0.01), while levels of RORC mRNA were significantly lower than in controls (P < 0.05). Levels of STAT3 mRNA did not vary significantly with treatment. After treatment with prednisone alone, proportions of Th17/CD4(+) cells and levels of IL-17A were significantly lower than in control cells, and proportions of Treg/CD4(+) cells and levels of IL-10 significantly higher than in controls (all P < 0.01). Our results suggest that prednisone may improve pregnancy outcomes by restoring immunological homeostasis through up-regulation of STAT5 and FOXP3, induction of DIC differentiation into Treg cells, inhibition of DIC differentiation into Th17 cells, reduction of IL-17A secretion and induction of IL-10 secretion. Impact Journals LLC 2017-10-31 /pmc/articles/PMC5731945/ /pubmed/29254235 http://dx.doi.org/10.18632/oncotarget.22188 Text en Copyright: © 2017 Fu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Fu, Xiao-Qian Cai, Jun-Ying Huang, Qian-Yi Li, Dong-Ju Li, Ning Li, Mu-Jun Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy |
title | Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy |
title_full | Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy |
title_fullStr | Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy |
title_full_unstemmed | Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy |
title_short | Prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy |
title_sort | prednisone may induce immunologic tolerance by activating the functions of decidual immune cells in early pregnancy |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731945/ https://www.ncbi.nlm.nih.gov/pubmed/29254235 http://dx.doi.org/10.18632/oncotarget.22188 |
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