Cargando…

Ag-doping regulates the cytotoxicity of TiO(2) nanoparticles via oxidative stress in human cancer cells

We investigated the anticancer potential of Ag-doped (0.5–5%) anatase TiO(2) NPs. Characterization study showed that dopant Ag was well-distributed on the surface of host TiO(2) NPs. Size (15 nm to 9 nm) and band gap energy (3.32 eV to 3.15 eV) of TiO(2) NPs were decreases with increasing the concen...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahamed, Maqusood, Khan, M. A. Majeed, Akhtar, Mohd Javed, Alhadlaq, Hisham A., Alshamsan, Aws
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732217/
https://www.ncbi.nlm.nih.gov/pubmed/29247182
http://dx.doi.org/10.1038/s41598-017-17559-9
_version_ 1783286643898187776
author Ahamed, Maqusood
Khan, M. A. Majeed
Akhtar, Mohd Javed
Alhadlaq, Hisham A.
Alshamsan, Aws
author_facet Ahamed, Maqusood
Khan, M. A. Majeed
Akhtar, Mohd Javed
Alhadlaq, Hisham A.
Alshamsan, Aws
author_sort Ahamed, Maqusood
collection PubMed
description We investigated the anticancer potential of Ag-doped (0.5–5%) anatase TiO(2) NPs. Characterization study showed that dopant Ag was well-distributed on the surface of host TiO(2) NPs. Size (15 nm to 9 nm) and band gap energy (3.32 eV to 3.15 eV) of TiO(2) NPs were decreases with increasing the concentration of Ag dopant. Biological studies demonstrated that Ag-doped TiO(2) NP-induced cytotoxicity and apoptosis in human liver cancer (HepG2) cells. The toxic intensity of TiO(2) NPs was increases with increasing the amount of Ag-doping. The Ag-doped TiO(2) NPs further found to provoke reactive oxygen species (ROS) generation and antioxidants depletion. Toxicity induced by Ag-doped TiO(2) NPs in HepG2 cells was efficiently abrogated by antioxidant N-acetyl-cysteine (ROS scavenger). We also found that Ag-doped TiO(2) NPs induced cytotoxicity and oxidative stress in human lung (A549) and breast (MCF-7) cancer cells. Interestingly, Ag-doped TiO(2) NPs did not cause much toxicity to normal cells such as primary rat hepatocytes and human lung fibroblasts. Overall, we found that Ag-doped TiO(2) NPs have potential to selectively kill cancer cells while sparing normal cells. This study warranted further research on anticancer potential of Ag-doped TiO(2) NPs in various types of cancer cells and in vivo models.
format Online
Article
Text
id pubmed-5732217
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-57322172017-12-21 Ag-doping regulates the cytotoxicity of TiO(2) nanoparticles via oxidative stress in human cancer cells Ahamed, Maqusood Khan, M. A. Majeed Akhtar, Mohd Javed Alhadlaq, Hisham A. Alshamsan, Aws Sci Rep Article We investigated the anticancer potential of Ag-doped (0.5–5%) anatase TiO(2) NPs. Characterization study showed that dopant Ag was well-distributed on the surface of host TiO(2) NPs. Size (15 nm to 9 nm) and band gap energy (3.32 eV to 3.15 eV) of TiO(2) NPs were decreases with increasing the concentration of Ag dopant. Biological studies demonstrated that Ag-doped TiO(2) NP-induced cytotoxicity and apoptosis in human liver cancer (HepG2) cells. The toxic intensity of TiO(2) NPs was increases with increasing the amount of Ag-doping. The Ag-doped TiO(2) NPs further found to provoke reactive oxygen species (ROS) generation and antioxidants depletion. Toxicity induced by Ag-doped TiO(2) NPs in HepG2 cells was efficiently abrogated by antioxidant N-acetyl-cysteine (ROS scavenger). We also found that Ag-doped TiO(2) NPs induced cytotoxicity and oxidative stress in human lung (A549) and breast (MCF-7) cancer cells. Interestingly, Ag-doped TiO(2) NPs did not cause much toxicity to normal cells such as primary rat hepatocytes and human lung fibroblasts. Overall, we found that Ag-doped TiO(2) NPs have potential to selectively kill cancer cells while sparing normal cells. This study warranted further research on anticancer potential of Ag-doped TiO(2) NPs in various types of cancer cells and in vivo models. Nature Publishing Group UK 2017-12-15 /pmc/articles/PMC5732217/ /pubmed/29247182 http://dx.doi.org/10.1038/s41598-017-17559-9 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ahamed, Maqusood
Khan, M. A. Majeed
Akhtar, Mohd Javed
Alhadlaq, Hisham A.
Alshamsan, Aws
Ag-doping regulates the cytotoxicity of TiO(2) nanoparticles via oxidative stress in human cancer cells
title Ag-doping regulates the cytotoxicity of TiO(2) nanoparticles via oxidative stress in human cancer cells
title_full Ag-doping regulates the cytotoxicity of TiO(2) nanoparticles via oxidative stress in human cancer cells
title_fullStr Ag-doping regulates the cytotoxicity of TiO(2) nanoparticles via oxidative stress in human cancer cells
title_full_unstemmed Ag-doping regulates the cytotoxicity of TiO(2) nanoparticles via oxidative stress in human cancer cells
title_short Ag-doping regulates the cytotoxicity of TiO(2) nanoparticles via oxidative stress in human cancer cells
title_sort ag-doping regulates the cytotoxicity of tio(2) nanoparticles via oxidative stress in human cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732217/
https://www.ncbi.nlm.nih.gov/pubmed/29247182
http://dx.doi.org/10.1038/s41598-017-17559-9
work_keys_str_mv AT ahamedmaqusood agdopingregulatesthecytotoxicityoftio2nanoparticlesviaoxidativestressinhumancancercells
AT khanmamajeed agdopingregulatesthecytotoxicityoftio2nanoparticlesviaoxidativestressinhumancancercells
AT akhtarmohdjaved agdopingregulatesthecytotoxicityoftio2nanoparticlesviaoxidativestressinhumancancercells
AT alhadlaqhishama agdopingregulatesthecytotoxicityoftio2nanoparticlesviaoxidativestressinhumancancercells
AT alshamsanaws agdopingregulatesthecytotoxicityoftio2nanoparticlesviaoxidativestressinhumancancercells