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Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating miRNA-27b: In Vitro and In Vivo Studies

This research aimed to evaluate the potential therapeutic effects of Rg3 on endometriosis and identify target miRNAs. We designed an in vitro study using human endometrial stromal cells (HESCs) obtained from patients with endometriosis and an in vivo study using mouse models. HESCs were treated with...

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Autores principales: Kim, Min Kyoung, Lee, Seung Kyun, Park, Ji Hyun, Lee, Jae Hoon, Yun, Bo Hyon, Park, Joo Hyun, Seo, Seok Kyo, Cho, SiHyun, Choi, Young Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732249/
https://www.ncbi.nlm.nih.gov/pubmed/29247225
http://dx.doi.org/10.1038/s41598-017-17956-0
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author Kim, Min Kyoung
Lee, Seung Kyun
Park, Ji Hyun
Lee, Jae Hoon
Yun, Bo Hyon
Park, Joo Hyun
Seo, Seok Kyo
Cho, SiHyun
Choi, Young Sik
author_facet Kim, Min Kyoung
Lee, Seung Kyun
Park, Ji Hyun
Lee, Jae Hoon
Yun, Bo Hyon
Park, Joo Hyun
Seo, Seok Kyo
Cho, SiHyun
Choi, Young Sik
author_sort Kim, Min Kyoung
collection PubMed
description This research aimed to evaluate the potential therapeutic effects of Rg3 on endometriosis and identify target miRNAs. We designed an in vitro study using human endometrial stromal cells (HESCs) obtained from patients with endometriosis and an in vivo study using mouse models. HESCs were treated with Rg3-enhanced red ginseng extract (Rg3E); real-time PCR and microarray profiling, transfection, and western blot were performed. Mouse endometriosis models were developed and supplemented with Rg3E for 8 weeks. Gross lesion size and fibrotic character were analyzed in the mouse models. RNA levels of Ki-67, col-1, CTGF, fibronectin, TGF-β1, MMP2 and MMP9 significantly decreased in HESCs after Rg3E treatment. Microarray analysis revealed downregulation of miR-27b-3p, which is related to fibrosis modulation. Expression of miR-27b-3p was significantly higher in HESCs from patients with endometriosis than that of controls, and Rg3E treatment significantly decreased its expression; the contraction and migration assay revealed significant reductions in both fibrosis and migration potential in Rg3E-treated HESCs from endometriosis patients. A decrease in size and fibrotic character of endometrial lesions from the Rg3E groups was observed in vivo. In conclusion, Rg3 effectively altered fibrotic properties of HESCs from patients with endometriosis, which is likely associated with miR-27b-3p modulation.
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spelling pubmed-57322492017-12-21 Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating miRNA-27b: In Vitro and In Vivo Studies Kim, Min Kyoung Lee, Seung Kyun Park, Ji Hyun Lee, Jae Hoon Yun, Bo Hyon Park, Joo Hyun Seo, Seok Kyo Cho, SiHyun Choi, Young Sik Sci Rep Article This research aimed to evaluate the potential therapeutic effects of Rg3 on endometriosis and identify target miRNAs. We designed an in vitro study using human endometrial stromal cells (HESCs) obtained from patients with endometriosis and an in vivo study using mouse models. HESCs were treated with Rg3-enhanced red ginseng extract (Rg3E); real-time PCR and microarray profiling, transfection, and western blot were performed. Mouse endometriosis models were developed and supplemented with Rg3E for 8 weeks. Gross lesion size and fibrotic character were analyzed in the mouse models. RNA levels of Ki-67, col-1, CTGF, fibronectin, TGF-β1, MMP2 and MMP9 significantly decreased in HESCs after Rg3E treatment. Microarray analysis revealed downregulation of miR-27b-3p, which is related to fibrosis modulation. Expression of miR-27b-3p was significantly higher in HESCs from patients with endometriosis than that of controls, and Rg3E treatment significantly decreased its expression; the contraction and migration assay revealed significant reductions in both fibrosis and migration potential in Rg3E-treated HESCs from endometriosis patients. A decrease in size and fibrotic character of endometrial lesions from the Rg3E groups was observed in vivo. In conclusion, Rg3 effectively altered fibrotic properties of HESCs from patients with endometriosis, which is likely associated with miR-27b-3p modulation. Nature Publishing Group UK 2017-12-15 /pmc/articles/PMC5732249/ /pubmed/29247225 http://dx.doi.org/10.1038/s41598-017-17956-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kim, Min Kyoung
Lee, Seung Kyun
Park, Ji Hyun
Lee, Jae Hoon
Yun, Bo Hyon
Park, Joo Hyun
Seo, Seok Kyo
Cho, SiHyun
Choi, Young Sik
Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating miRNA-27b: In Vitro and In Vivo Studies
title Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating miRNA-27b: In Vitro and In Vivo Studies
title_full Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating miRNA-27b: In Vitro and In Vivo Studies
title_fullStr Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating miRNA-27b: In Vitro and In Vivo Studies
title_full_unstemmed Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating miRNA-27b: In Vitro and In Vivo Studies
title_short Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating miRNA-27b: In Vitro and In Vivo Studies
title_sort ginsenoside rg3 decreases fibrotic and invasive nature of endometriosis by modulating mirna-27b: in vitro and in vivo studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732249/
https://www.ncbi.nlm.nih.gov/pubmed/29247225
http://dx.doi.org/10.1038/s41598-017-17956-0
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