Levetiracetam Affects Differentially Presynaptic Proteins in Rat Cerebral Cortex

Presynaptic proteins are potential therapeutic targets for epilepsy and other neurological diseases. We tested the hypothesis that chronic treatment with the SV2A ligand levetiracetam affects the expression of other presynaptic proteins. Results showed that in rat neocortex no significant difference...

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Autores principales: Marcotulli, Daniele, Fattorini, Giorgia, Bragina, Luca, Perugini, Jessica, Conti, Fiorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732259/
https://www.ncbi.nlm.nih.gov/pubmed/29311825
http://dx.doi.org/10.3389/fncel.2017.00389
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author Marcotulli, Daniele
Fattorini, Giorgia
Bragina, Luca
Perugini, Jessica
Conti, Fiorenzo
author_facet Marcotulli, Daniele
Fattorini, Giorgia
Bragina, Luca
Perugini, Jessica
Conti, Fiorenzo
author_sort Marcotulli, Daniele
collection PubMed
description Presynaptic proteins are potential therapeutic targets for epilepsy and other neurological diseases. We tested the hypothesis that chronic treatment with the SV2A ligand levetiracetam affects the expression of other presynaptic proteins. Results showed that in rat neocortex no significant difference was detected in SV2A protein levels in levetiracetam treated animals compared to controls, whereas levetiracetam post-transcriptionally decreased several vesicular proteins and increased LRRK2, without any change in mRNA levels. Analysis of SV2A interactome indicates that the presynaptic proteins regulation induced by levetiracetam reported here is mediated by this interactome, and suggests that LRRK2 plays a role in forging the pattern of effects.
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spelling pubmed-57322592018-01-08 Levetiracetam Affects Differentially Presynaptic Proteins in Rat Cerebral Cortex Marcotulli, Daniele Fattorini, Giorgia Bragina, Luca Perugini, Jessica Conti, Fiorenzo Front Cell Neurosci Neuroscience Presynaptic proteins are potential therapeutic targets for epilepsy and other neurological diseases. We tested the hypothesis that chronic treatment with the SV2A ligand levetiracetam affects the expression of other presynaptic proteins. Results showed that in rat neocortex no significant difference was detected in SV2A protein levels in levetiracetam treated animals compared to controls, whereas levetiracetam post-transcriptionally decreased several vesicular proteins and increased LRRK2, without any change in mRNA levels. Analysis of SV2A interactome indicates that the presynaptic proteins regulation induced by levetiracetam reported here is mediated by this interactome, and suggests that LRRK2 plays a role in forging the pattern of effects. Frontiers Media S.A. 2017-12-11 /pmc/articles/PMC5732259/ /pubmed/29311825 http://dx.doi.org/10.3389/fncel.2017.00389 Text en Copyright © 2017 Marcotulli, Fattorini, Bragina, Perugini and Conti. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Marcotulli, Daniele
Fattorini, Giorgia
Bragina, Luca
Perugini, Jessica
Conti, Fiorenzo
Levetiracetam Affects Differentially Presynaptic Proteins in Rat Cerebral Cortex
title Levetiracetam Affects Differentially Presynaptic Proteins in Rat Cerebral Cortex
title_full Levetiracetam Affects Differentially Presynaptic Proteins in Rat Cerebral Cortex
title_fullStr Levetiracetam Affects Differentially Presynaptic Proteins in Rat Cerebral Cortex
title_full_unstemmed Levetiracetam Affects Differentially Presynaptic Proteins in Rat Cerebral Cortex
title_short Levetiracetam Affects Differentially Presynaptic Proteins in Rat Cerebral Cortex
title_sort levetiracetam affects differentially presynaptic proteins in rat cerebral cortex
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732259/
https://www.ncbi.nlm.nih.gov/pubmed/29311825
http://dx.doi.org/10.3389/fncel.2017.00389
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