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Plastination of macroparasites: An eco-friendly method of long-term preservation

AIM: Preservation of macroparasites by infiltrating the polymer in the tissues can defy the inherited shortcoming of classical wet preservation method. MATERIALS AND METHODS: Preservation was done by infiltrating the melamine alone or with xylene (MX)/chloroform (MC)/turpentine oil (MT) in 1:1 and h...

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Autores principales: Kumar, Niranjan, Das, Bhupamani, Solanki, Jayesh B., Jadav, Mehul M., Menaka, Ramasamy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732349/
https://www.ncbi.nlm.nih.gov/pubmed/29263605
http://dx.doi.org/10.14202/vetworld.2017.1394-1400
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author Kumar, Niranjan
Das, Bhupamani
Solanki, Jayesh B.
Jadav, Mehul M.
Menaka, Ramasamy
author_facet Kumar, Niranjan
Das, Bhupamani
Solanki, Jayesh B.
Jadav, Mehul M.
Menaka, Ramasamy
author_sort Kumar, Niranjan
collection PubMed
description AIM: Preservation of macroparasites by infiltrating the polymer in the tissues can defy the inherited shortcoming of classical wet preservation method. MATERIALS AND METHODS: Preservation was done by infiltrating the melamine alone or with xylene (MX)/chloroform (MC)/turpentine oil (MT) in 1:1 and hardener (MH) in 9:1 ratio in the tissues of the gross specimen of the animal parasites. RESULTS: The plastinated models withstand the process of microbial decomposition, and remain intact in the environmental conditions. The polymer mixture resists the entry of the water molecule, and model dried just after taking out it from the water tank. Overall, the plastinated parasites were dry, non-sticky, glossy, odorless, chemical free, and harmless, to some extent flexible, with detectable morphological structure, and retain their natural form but lost their natural color. Full marks were assigned to the degree of dryness, non-stickiness, and odorlessness to the model plastinated in different solutions on a five-point scale. For flexibility, the score was 1.2, 2.2, and 2.4 for the plastinated model in melamine/MH, MX/MC, and MT solutions, respectively. The average score of glossiness was 4.6 and 5 for the specimen plastinated in melamine/MH and MX/MC/MT solutions, respectively. The degree of dryness, glossiness, stickiness, and flexibility varies non-significantly, with the polymer mixtures used. CONCLUSION: The prepared model can be used to educate the students/general mass population.
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spelling pubmed-57323492017-12-20 Plastination of macroparasites: An eco-friendly method of long-term preservation Kumar, Niranjan Das, Bhupamani Solanki, Jayesh B. Jadav, Mehul M. Menaka, Ramasamy Vet World Research Article AIM: Preservation of macroparasites by infiltrating the polymer in the tissues can defy the inherited shortcoming of classical wet preservation method. MATERIALS AND METHODS: Preservation was done by infiltrating the melamine alone or with xylene (MX)/chloroform (MC)/turpentine oil (MT) in 1:1 and hardener (MH) in 9:1 ratio in the tissues of the gross specimen of the animal parasites. RESULTS: The plastinated models withstand the process of microbial decomposition, and remain intact in the environmental conditions. The polymer mixture resists the entry of the water molecule, and model dried just after taking out it from the water tank. Overall, the plastinated parasites were dry, non-sticky, glossy, odorless, chemical free, and harmless, to some extent flexible, with detectable morphological structure, and retain their natural form but lost their natural color. Full marks were assigned to the degree of dryness, non-stickiness, and odorlessness to the model plastinated in different solutions on a five-point scale. For flexibility, the score was 1.2, 2.2, and 2.4 for the plastinated model in melamine/MH, MX/MC, and MT solutions, respectively. The average score of glossiness was 4.6 and 5 for the specimen plastinated in melamine/MH and MX/MC/MT solutions, respectively. The degree of dryness, glossiness, stickiness, and flexibility varies non-significantly, with the polymer mixtures used. CONCLUSION: The prepared model can be used to educate the students/general mass population. Veterinary World 2017-11 2017-11-29 /pmc/articles/PMC5732349/ /pubmed/29263605 http://dx.doi.org/10.14202/vetworld.2017.1394-1400 Text en Copyright: © Kumar, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kumar, Niranjan
Das, Bhupamani
Solanki, Jayesh B.
Jadav, Mehul M.
Menaka, Ramasamy
Plastination of macroparasites: An eco-friendly method of long-term preservation
title Plastination of macroparasites: An eco-friendly method of long-term preservation
title_full Plastination of macroparasites: An eco-friendly method of long-term preservation
title_fullStr Plastination of macroparasites: An eco-friendly method of long-term preservation
title_full_unstemmed Plastination of macroparasites: An eco-friendly method of long-term preservation
title_short Plastination of macroparasites: An eco-friendly method of long-term preservation
title_sort plastination of macroparasites: an eco-friendly method of long-term preservation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732349/
https://www.ncbi.nlm.nih.gov/pubmed/29263605
http://dx.doi.org/10.14202/vetworld.2017.1394-1400
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