Cargando…

Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer

BACKGROUND: The significance of PLK1 (polo-like kinase 1) has become increasingly essential as both a biomarker and a target for cancer treatment. Here, we aimed to determine the downstream genes of PLK1 and their effects on the carcinogenesis and progression of bladder cancer. METHODS: Specific siR...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Zhe, Zhang, Guojun, Gao, Zhipeng, Li, Shiguang, Li, Zeliang, Bi, Jianbin, Liu, Xiankui, Li, Zhenhua, Kong, Chuize
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732388/
https://www.ncbi.nlm.nih.gov/pubmed/29246203
http://dx.doi.org/10.1186/s12885-017-3884-2
_version_ 1783286685356785664
author Zhang, Zhe
Zhang, Guojun
Gao, Zhipeng
Li, Shiguang
Li, Zeliang
Bi, Jianbin
Liu, Xiankui
Li, Zhenhua
Kong, Chuize
author_facet Zhang, Zhe
Zhang, Guojun
Gao, Zhipeng
Li, Shiguang
Li, Zeliang
Bi, Jianbin
Liu, Xiankui
Li, Zhenhua
Kong, Chuize
author_sort Zhang, Zhe
collection PubMed
description BACKGROUND: The significance of PLK1 (polo-like kinase 1) has become increasingly essential as both a biomarker and a target for cancer treatment. Here, we aimed to determine the downstream genes of PLK1 and their effects on the carcinogenesis and progression of bladder cancer. METHODS: Specific siRNA was utilized to silence the target gene expression. The cell proliferation, invasion and migration of bladder cancer cells by MTT assay, BrdU assay and transwell assay. The differential expression genes were identified using Affymetrix HTA2.0 Array. The KEGG, GO and STRING analysis were used to analyze the signaling pathway and protein-protein interaction. Spearman analysis was used to analyze the correlation between protein and protein, between protein and clincopathologic characteristics. RESULTS: PLK1 siRNA hindered the proliferation, invasion and migration of bladder cancer cells, as determined by the MTT, BrdU and transwell assays. A total of 561 differentially expressed genes were identified using an Affymetrix HTA2.0 Array in PLK1 knockdown T24 cells. According to KEGG, GO and STRING analysis, five key genes (BUB1B, CCNB1, CDC25A, FBXO5, NDC80) were determined to be involved in cell proliferation, invasion and migration. PLK1 knockdown decreased BUB1B, CCNB1, CDC25A and NDC80 expressions but increased FBXO5 expression. BUB1B, CCNB1, CDC25A and NDC80 were positively correlated with cell proliferation, invasion, migration and PLK1 expression in tissues, but FBXO5 was negatively correlated with each of those factors. The results showed that the five genes expressions were significantly correlation with the PLK1 expression in normal bladder tissues and bladder cancer tissues. Four of them (BUB1B, CCNB1, CDC25A, NDC80) were obviously positive correlations with pT stage and metastasis. But FBXO5 was negative correlated with pT stage and metastasis. Furthermore, significant correlations were found between CCNB1 or CDC25A or NDC80 and histological grade; between BUB1B or NDC80 and recurrence. CONCLUSION: Five downstream genes of PLK1 were associated with the regulation of cell proliferation, invasion and migration in bladder cancer. Furthermore, these genes may play important roles in bladder cancer and become important biomarkers and targets for cancer treatment. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-017-3884-2) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5732388
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-57323882017-12-21 Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer Zhang, Zhe Zhang, Guojun Gao, Zhipeng Li, Shiguang Li, Zeliang Bi, Jianbin Liu, Xiankui Li, Zhenhua Kong, Chuize BMC Cancer Research Article BACKGROUND: The significance of PLK1 (polo-like kinase 1) has become increasingly essential as both a biomarker and a target for cancer treatment. Here, we aimed to determine the downstream genes of PLK1 and their effects on the carcinogenesis and progression of bladder cancer. METHODS: Specific siRNA was utilized to silence the target gene expression. The cell proliferation, invasion and migration of bladder cancer cells by MTT assay, BrdU assay and transwell assay. The differential expression genes were identified using Affymetrix HTA2.0 Array. The KEGG, GO and STRING analysis were used to analyze the signaling pathway and protein-protein interaction. Spearman analysis was used to analyze the correlation between protein and protein, between protein and clincopathologic characteristics. RESULTS: PLK1 siRNA hindered the proliferation, invasion and migration of bladder cancer cells, as determined by the MTT, BrdU and transwell assays. A total of 561 differentially expressed genes were identified using an Affymetrix HTA2.0 Array in PLK1 knockdown T24 cells. According to KEGG, GO and STRING analysis, five key genes (BUB1B, CCNB1, CDC25A, FBXO5, NDC80) were determined to be involved in cell proliferation, invasion and migration. PLK1 knockdown decreased BUB1B, CCNB1, CDC25A and NDC80 expressions but increased FBXO5 expression. BUB1B, CCNB1, CDC25A and NDC80 were positively correlated with cell proliferation, invasion, migration and PLK1 expression in tissues, but FBXO5 was negatively correlated with each of those factors. The results showed that the five genes expressions were significantly correlation with the PLK1 expression in normal bladder tissues and bladder cancer tissues. Four of them (BUB1B, CCNB1, CDC25A, NDC80) were obviously positive correlations with pT stage and metastasis. But FBXO5 was negative correlated with pT stage and metastasis. Furthermore, significant correlations were found between CCNB1 or CDC25A or NDC80 and histological grade; between BUB1B or NDC80 and recurrence. CONCLUSION: Five downstream genes of PLK1 were associated with the regulation of cell proliferation, invasion and migration in bladder cancer. Furthermore, these genes may play important roles in bladder cancer and become important biomarkers and targets for cancer treatment. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-017-3884-2) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-16 /pmc/articles/PMC5732388/ /pubmed/29246203 http://dx.doi.org/10.1186/s12885-017-3884-2 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Zhe
Zhang, Guojun
Gao, Zhipeng
Li, Shiguang
Li, Zeliang
Bi, Jianbin
Liu, Xiankui
Li, Zhenhua
Kong, Chuize
Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer
title Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer
title_full Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer
title_fullStr Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer
title_full_unstemmed Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer
title_short Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer
title_sort comprehensive analysis of differentially expressed genes associated with plk1 in bladder cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732388/
https://www.ncbi.nlm.nih.gov/pubmed/29246203
http://dx.doi.org/10.1186/s12885-017-3884-2
work_keys_str_mv AT zhangzhe comprehensiveanalysisofdifferentiallyexpressedgenesassociatedwithplk1inbladdercancer
AT zhangguojun comprehensiveanalysisofdifferentiallyexpressedgenesassociatedwithplk1inbladdercancer
AT gaozhipeng comprehensiveanalysisofdifferentiallyexpressedgenesassociatedwithplk1inbladdercancer
AT lishiguang comprehensiveanalysisofdifferentiallyexpressedgenesassociatedwithplk1inbladdercancer
AT lizeliang comprehensiveanalysisofdifferentiallyexpressedgenesassociatedwithplk1inbladdercancer
AT bijianbin comprehensiveanalysisofdifferentiallyexpressedgenesassociatedwithplk1inbladdercancer
AT liuxiankui comprehensiveanalysisofdifferentiallyexpressedgenesassociatedwithplk1inbladdercancer
AT lizhenhua comprehensiveanalysisofdifferentiallyexpressedgenesassociatedwithplk1inbladdercancer
AT kongchuize comprehensiveanalysisofdifferentiallyexpressedgenesassociatedwithplk1inbladdercancer