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HNRNPK inhibits gastric cancer cell proliferation through p53/p21/CCND1 pathway

Gastric cancer (GC) is one of the most common human cancers. The molecular mechanisms underlying GC carcinogenesis and progression are still not well understood. In this study, we showed that heterogeneous nuclear ribonucleoprotein K (HNRNPK) was an effective prognostic marker for GC patients especi...

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Autores principales: Huang, Hao, Han, Yong, Yang, Xingjiu, Li, Mengyuan, Zhu, Ruimin, Hu, Juanjuan, Zhang, Xiaowei, Wei, Rongfei, Li, Kejuan, Gao, Ran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732733/
https://www.ncbi.nlm.nih.gov/pubmed/29262567
http://dx.doi.org/10.18632/oncotarget.21873
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author Huang, Hao
Han, Yong
Yang, Xingjiu
Li, Mengyuan
Zhu, Ruimin
Hu, Juanjuan
Zhang, Xiaowei
Wei, Rongfei
Li, Kejuan
Gao, Ran
author_facet Huang, Hao
Han, Yong
Yang, Xingjiu
Li, Mengyuan
Zhu, Ruimin
Hu, Juanjuan
Zhang, Xiaowei
Wei, Rongfei
Li, Kejuan
Gao, Ran
author_sort Huang, Hao
collection PubMed
description Gastric cancer (GC) is one of the most common human cancers. The molecular mechanisms underlying GC carcinogenesis and progression are still not well understood. In this study, we showed that heterogeneous nuclear ribonucleoprotein K (HNRNPK) was an effective prognostic marker for GC patients especially in early stage. Overexpression of HNRNPK can retard tumor cell proliferation and colony formation in vitro and inhibit tumor growth in vivo through p53/p21/CCND1 axis. Bioinformatics analyses indicated that HNRNPK associated genes were enriched in cell cycle and DNA replication process. Protein-protein interaction network showed that HNRNPK was physically interacted with p53, p21 and other cancer related genes. Besides, GSEA showed that HNRNPK expression was positively correlated with GAMMA radiation response and DNA repair, while negatively correlated with angiogenesis, TGF-β and Hedgehog pathway activation. Finally, several chemicals including Glycine that may repress GC progression through upregulating HNRNPK are suggested. Our study demonstrated that HNRNPK may play as a tumor suppressor in gastric cancer and could be a potential therapeutic target for GC.
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spelling pubmed-57327332017-12-19 HNRNPK inhibits gastric cancer cell proliferation through p53/p21/CCND1 pathway Huang, Hao Han, Yong Yang, Xingjiu Li, Mengyuan Zhu, Ruimin Hu, Juanjuan Zhang, Xiaowei Wei, Rongfei Li, Kejuan Gao, Ran Oncotarget Research Paper Gastric cancer (GC) is one of the most common human cancers. The molecular mechanisms underlying GC carcinogenesis and progression are still not well understood. In this study, we showed that heterogeneous nuclear ribonucleoprotein K (HNRNPK) was an effective prognostic marker for GC patients especially in early stage. Overexpression of HNRNPK can retard tumor cell proliferation and colony formation in vitro and inhibit tumor growth in vivo through p53/p21/CCND1 axis. Bioinformatics analyses indicated that HNRNPK associated genes were enriched in cell cycle and DNA replication process. Protein-protein interaction network showed that HNRNPK was physically interacted with p53, p21 and other cancer related genes. Besides, GSEA showed that HNRNPK expression was positively correlated with GAMMA radiation response and DNA repair, while negatively correlated with angiogenesis, TGF-β and Hedgehog pathway activation. Finally, several chemicals including Glycine that may repress GC progression through upregulating HNRNPK are suggested. Our study demonstrated that HNRNPK may play as a tumor suppressor in gastric cancer and could be a potential therapeutic target for GC. Impact Journals LLC 2017-10-17 /pmc/articles/PMC5732733/ /pubmed/29262567 http://dx.doi.org/10.18632/oncotarget.21873 Text en Copyright: © 2017 Huang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Huang, Hao
Han, Yong
Yang, Xingjiu
Li, Mengyuan
Zhu, Ruimin
Hu, Juanjuan
Zhang, Xiaowei
Wei, Rongfei
Li, Kejuan
Gao, Ran
HNRNPK inhibits gastric cancer cell proliferation through p53/p21/CCND1 pathway
title HNRNPK inhibits gastric cancer cell proliferation through p53/p21/CCND1 pathway
title_full HNRNPK inhibits gastric cancer cell proliferation through p53/p21/CCND1 pathway
title_fullStr HNRNPK inhibits gastric cancer cell proliferation through p53/p21/CCND1 pathway
title_full_unstemmed HNRNPK inhibits gastric cancer cell proliferation through p53/p21/CCND1 pathway
title_short HNRNPK inhibits gastric cancer cell proliferation through p53/p21/CCND1 pathway
title_sort hnrnpk inhibits gastric cancer cell proliferation through p53/p21/ccnd1 pathway
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732733/
https://www.ncbi.nlm.nih.gov/pubmed/29262567
http://dx.doi.org/10.18632/oncotarget.21873
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