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Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer
BACKGROUND: Immune checkpoint inhibitors are reported to be effective in patients with brain metastases. However, detailed characteristics of the brain metastasis immune microenvironment remain unexplored. RESULTS: The median tumor-infiltrating lymphocyte (TIL) category in brain metastases was 5% (1...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732758/ https://www.ncbi.nlm.nih.gov/pubmed/29262592 http://dx.doi.org/10.18632/oncotarget.22110 |
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author | Ogiya, Rin Niikura, Naoki Kumaki, Nobue Yasojima, Hiroyuki Iwasa, Tsutomu Kanbayashi, Chizuko Oshitanai, Risa Tsuneizumi, Michiko Watanabe, Ken-ichi Matsui, Akira Fujisawa, Tomomi Saji, Shigehira Masuda, Norikazu Tokuda, Yutaka Iwata, Hiroji |
author_facet | Ogiya, Rin Niikura, Naoki Kumaki, Nobue Yasojima, Hiroyuki Iwasa, Tsutomu Kanbayashi, Chizuko Oshitanai, Risa Tsuneizumi, Michiko Watanabe, Ken-ichi Matsui, Akira Fujisawa, Tomomi Saji, Shigehira Masuda, Norikazu Tokuda, Yutaka Iwata, Hiroji |
author_sort | Ogiya, Rin |
collection | PubMed |
description | BACKGROUND: Immune checkpoint inhibitors are reported to be effective in patients with brain metastases. However, detailed characteristics of the brain metastasis immune microenvironment remain unexplored. RESULTS: The median tumor-infiltrating lymphocyte (TIL) category in brain metastases was 5% (1–70%). In 46 pair-matched samples, the percentages of TILs were significantly higher in primary breast tumors than in brain metastases (paired t-test, P < 0.01). The numbers of CD4/CD8/Foxp3-positive cells were significantly higher in primary breast tumors than in brain metastases (paired t-test, P < 0.05 for all antibodies). In patients with triple-negative breast cancer specifically, low TIL numbers were associated with significantly shorter overall survival compared to high TIL numbers (log-rank test, P = 0.04). MATERIALS AND METHODS: We retrospectively identified 107 patients with breast cancer and brain metastases who had undergone surgery between 2001 and 2012 at 8 institutions, and collected 191 samples including brain metastases alone and primary tumors with pair-matched brain metastasis samples. Hematoxylin and eosin-stained slides were evaluated for TILs and categorized according to the extent of staining. Immunohistochemistry for CD4, CD8, Foxp3, PD-L1, PD-L2, and HLA class I was also performed. CONCLUSIONS: There are significantly fewer TILs in brain metastases than in primary breast tumors. |
format | Online Article Text |
id | pubmed-5732758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57327582017-12-19 Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer Ogiya, Rin Niikura, Naoki Kumaki, Nobue Yasojima, Hiroyuki Iwasa, Tsutomu Kanbayashi, Chizuko Oshitanai, Risa Tsuneizumi, Michiko Watanabe, Ken-ichi Matsui, Akira Fujisawa, Tomomi Saji, Shigehira Masuda, Norikazu Tokuda, Yutaka Iwata, Hiroji Oncotarget Research Paper BACKGROUND: Immune checkpoint inhibitors are reported to be effective in patients with brain metastases. However, detailed characteristics of the brain metastasis immune microenvironment remain unexplored. RESULTS: The median tumor-infiltrating lymphocyte (TIL) category in brain metastases was 5% (1–70%). In 46 pair-matched samples, the percentages of TILs were significantly higher in primary breast tumors than in brain metastases (paired t-test, P < 0.01). The numbers of CD4/CD8/Foxp3-positive cells were significantly higher in primary breast tumors than in brain metastases (paired t-test, P < 0.05 for all antibodies). In patients with triple-negative breast cancer specifically, low TIL numbers were associated with significantly shorter overall survival compared to high TIL numbers (log-rank test, P = 0.04). MATERIALS AND METHODS: We retrospectively identified 107 patients with breast cancer and brain metastases who had undergone surgery between 2001 and 2012 at 8 institutions, and collected 191 samples including brain metastases alone and primary tumors with pair-matched brain metastasis samples. Hematoxylin and eosin-stained slides were evaluated for TILs and categorized according to the extent of staining. Immunohistochemistry for CD4, CD8, Foxp3, PD-L1, PD-L2, and HLA class I was also performed. CONCLUSIONS: There are significantly fewer TILs in brain metastases than in primary breast tumors. Impact Journals LLC 2017-10-27 /pmc/articles/PMC5732758/ /pubmed/29262592 http://dx.doi.org/10.18632/oncotarget.22110 Text en Copyright: © 2017 Ogiya et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Ogiya, Rin Niikura, Naoki Kumaki, Nobue Yasojima, Hiroyuki Iwasa, Tsutomu Kanbayashi, Chizuko Oshitanai, Risa Tsuneizumi, Michiko Watanabe, Ken-ichi Matsui, Akira Fujisawa, Tomomi Saji, Shigehira Masuda, Norikazu Tokuda, Yutaka Iwata, Hiroji Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer |
title | Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer |
title_full | Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer |
title_fullStr | Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer |
title_full_unstemmed | Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer |
title_short | Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer |
title_sort | comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732758/ https://www.ncbi.nlm.nih.gov/pubmed/29262592 http://dx.doi.org/10.18632/oncotarget.22110 |
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