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Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis

To enhance nucleoside production in Hirsutella sinensis, the biosynthetic pathways of purine and pyrimidine nucleosides were constructed and verified. The differential expression analysis showed that purine nucleoside phosphorylase, inosine monophosphate dehydrogenase, and guanosine monophosphate sy...

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Autores principales: Liu, Zhi-Qiang, Zhang, Bo, Lin, Shan, Baker, Peter James, Chen, Mao-Sheng, Xue, Ya-Ping, Wu, Hui, Xu, Feng, Yuan, Shui-Jin, Teng, Yi, Wu, Ling-Fang, Zheng, Yu-Guo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733210/
https://www.ncbi.nlm.nih.gov/pubmed/29333435
http://dx.doi.org/10.1155/2017/2520347
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author Liu, Zhi-Qiang
Zhang, Bo
Lin, Shan
Baker, Peter James
Chen, Mao-Sheng
Xue, Ya-Ping
Wu, Hui
Xu, Feng
Yuan, Shui-Jin
Teng, Yi
Wu, Ling-Fang
Zheng, Yu-Guo
author_facet Liu, Zhi-Qiang
Zhang, Bo
Lin, Shan
Baker, Peter James
Chen, Mao-Sheng
Xue, Ya-Ping
Wu, Hui
Xu, Feng
Yuan, Shui-Jin
Teng, Yi
Wu, Ling-Fang
Zheng, Yu-Guo
author_sort Liu, Zhi-Qiang
collection PubMed
description To enhance nucleoside production in Hirsutella sinensis, the biosynthetic pathways of purine and pyrimidine nucleosides were constructed and verified. The differential expression analysis showed that purine nucleoside phosphorylase, inosine monophosphate dehydrogenase, and guanosine monophosphate synthase genes involved in purine nucleotide biosynthesis were significantly upregulated 16.56-fold, 8-fold, and 5.43-fold, respectively. Moreover, dihydroorotate dehydrogenase, uridine nucleosidase, uridine/cytidine monophosphate kinase, and inosine triphosphate pyrophosphatase genes participating in pyrimidine nucleoside biosynthesis were upregulated 4.53-fold, 10.63-fold, 4.26-fold, and 5.98-fold, respectively. To enhance the nucleoside production, precursors for synthesis of nucleosides were added based on the analysis of biosynthetic pathways. Uridine and cytidine contents, respectively, reached 5.04 mg/g and 3.54 mg/g when adding 2 mg/mL of ribose, resulting in an increase of 28.6% and 296% compared with the control, respectively. Meanwhile, uridine and cytidine contents, respectively, reached 10.83 mg/g 2.12 mg/g when adding 0.3 mg/mL of uracil, leading to an increase of 176.3% and 137.1%, respectively. This report indicated that fermentation regulation was an effective way to enhance the nucleoside production in H. sinensis based on biosynthetic pathway analysis.
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spelling pubmed-57332102018-01-14 Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis Liu, Zhi-Qiang Zhang, Bo Lin, Shan Baker, Peter James Chen, Mao-Sheng Xue, Ya-Ping Wu, Hui Xu, Feng Yuan, Shui-Jin Teng, Yi Wu, Ling-Fang Zheng, Yu-Guo Biomed Res Int Research Article To enhance nucleoside production in Hirsutella sinensis, the biosynthetic pathways of purine and pyrimidine nucleosides were constructed and verified. The differential expression analysis showed that purine nucleoside phosphorylase, inosine monophosphate dehydrogenase, and guanosine monophosphate synthase genes involved in purine nucleotide biosynthesis were significantly upregulated 16.56-fold, 8-fold, and 5.43-fold, respectively. Moreover, dihydroorotate dehydrogenase, uridine nucleosidase, uridine/cytidine monophosphate kinase, and inosine triphosphate pyrophosphatase genes participating in pyrimidine nucleoside biosynthesis were upregulated 4.53-fold, 10.63-fold, 4.26-fold, and 5.98-fold, respectively. To enhance the nucleoside production, precursors for synthesis of nucleosides were added based on the analysis of biosynthetic pathways. Uridine and cytidine contents, respectively, reached 5.04 mg/g and 3.54 mg/g when adding 2 mg/mL of ribose, resulting in an increase of 28.6% and 296% compared with the control, respectively. Meanwhile, uridine and cytidine contents, respectively, reached 10.83 mg/g 2.12 mg/g when adding 0.3 mg/mL of uracil, leading to an increase of 176.3% and 137.1%, respectively. This report indicated that fermentation regulation was an effective way to enhance the nucleoside production in H. sinensis based on biosynthetic pathway analysis. Hindawi 2017 2017-11-29 /pmc/articles/PMC5733210/ /pubmed/29333435 http://dx.doi.org/10.1155/2017/2520347 Text en Copyright © 2017 Zhi-Qiang Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Zhi-Qiang
Zhang, Bo
Lin, Shan
Baker, Peter James
Chen, Mao-Sheng
Xue, Ya-Ping
Wu, Hui
Xu, Feng
Yuan, Shui-Jin
Teng, Yi
Wu, Ling-Fang
Zheng, Yu-Guo
Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis
title Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis
title_full Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis
title_fullStr Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis
title_full_unstemmed Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis
title_short Enhancement of Nucleoside Production in Hirsutella sinensis Based on Biosynthetic Pathway Analysis
title_sort enhancement of nucleoside production in hirsutella sinensis based on biosynthetic pathway analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733210/
https://www.ncbi.nlm.nih.gov/pubmed/29333435
http://dx.doi.org/10.1155/2017/2520347
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