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In vitro reconstitution of chaperone-mediated human RISC assembly

To silence target mRNAs, small RNAs and Argonaute (Ago) proteins need to be assembled into RNA-induced silencing complexes (RISCs). Although the assembly of Drosophila melanogaster RISC was recently reconstituted by Ago2, the Dicer-2/R2D2 heterodimer, and five chaperone proteins, the absence of a re...

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Detalles Bibliográficos
Autores principales:	Naruse, Ken, Matsuura-Suzuki, Eriko, Watanabe, Mariko, Iwasaki, Shintaro, Tomari, Yukihide
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2018
Materias:	Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733571/ https://www.ncbi.nlm.nih.gov/pubmed/28971854 http://dx.doi.org/10.1261/rna.063891.117

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