Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants

Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostri...

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Detalles Bibliográficos
Autores principales: Grosse-Honebrink, Alexander, Schwarz, Katrin M., Wang, Hengzheng, Minton, Nigel P., Zhang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5734229/
https://www.ncbi.nlm.nih.gov/pubmed/28899647
http://dx.doi.org/10.1016/j.anaerobe.2017.09.001
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author Grosse-Honebrink, Alexander
Schwarz, Katrin M.
Wang, Hengzheng
Minton, Nigel P.
Zhang, Ying
author_facet Grosse-Honebrink, Alexander
Schwarz, Katrin M.
Wang, Hengzheng
Minton, Nigel P.
Zhang, Ying
author_sort Grosse-Honebrink, Alexander
collection PubMed
description Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostridium pasteurianum by three to four orders of magnitude.
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spelling pubmed-57342292017-12-21 Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants Grosse-Honebrink, Alexander Schwarz, Katrin M. Wang, Hengzheng Minton, Nigel P. Zhang, Ying Anaerobe Article Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostridium pasteurianum by three to four orders of magnitude. Academic Press 2017-12 /pmc/articles/PMC5734229/ /pubmed/28899647 http://dx.doi.org/10.1016/j.anaerobe.2017.09.001 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grosse-Honebrink, Alexander
Schwarz, Katrin M.
Wang, Hengzheng
Minton, Nigel P.
Zhang, Ying
Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
title Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
title_full Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
title_fullStr Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
title_full_unstemmed Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
title_short Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
title_sort improving gene transfer in clostridium pasteurianum through the isolation of rare hypertransformable variants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5734229/
https://www.ncbi.nlm.nih.gov/pubmed/28899647
http://dx.doi.org/10.1016/j.anaerobe.2017.09.001
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