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Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostri...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5734229/ https://www.ncbi.nlm.nih.gov/pubmed/28899647 http://dx.doi.org/10.1016/j.anaerobe.2017.09.001 |