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Genetic Analysis of Mu and Kappa Opioid Receptor and COMT Enzyme in Cancer Pain Tunisian Patients Under Opioid Treatment

BACKGROUND: Pain and its opioid treatments are complex measurable traits. Responses to morphine in terms of pain control is likely to be determined by many factors, including the underlying pain sensitivity of the patient, along with nature and extent of the painful process, concomitant medications,...

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Autores principales: CHATTI, Imen, WOILLARD, Jean-Baptiste, MILI, Amira, CREVEAUX, Isabelle, BEN CHARFEDDINE, Ilhem, FEKI, Jihène, LANGLAIS, Sarah, BEN FATMA, Leila, SAAD, Ali, GRIBAA, Moez, LIBERT, Frédéric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5734971/
https://www.ncbi.nlm.nih.gov/pubmed/29259946
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author CHATTI, Imen
WOILLARD, Jean-Baptiste
MILI, Amira
CREVEAUX, Isabelle
BEN CHARFEDDINE, Ilhem
FEKI, Jihène
LANGLAIS, Sarah
BEN FATMA, Leila
SAAD, Ali
GRIBAA, Moez
LIBERT, Frédéric
author_facet CHATTI, Imen
WOILLARD, Jean-Baptiste
MILI, Amira
CREVEAUX, Isabelle
BEN CHARFEDDINE, Ilhem
FEKI, Jihène
LANGLAIS, Sarah
BEN FATMA, Leila
SAAD, Ali
GRIBAA, Moez
LIBERT, Frédéric
author_sort CHATTI, Imen
collection PubMed
description BACKGROUND: Pain and its opioid treatments are complex measurable traits. Responses to morphine in terms of pain control is likely to be determined by many factors, including the underlying pain sensitivity of the patient, along with nature and extent of the painful process, concomitant medications, genetic and other clinical and environmental factors. This study investigated genetic polymorphisms implicated in the inter-individual pain response variability to opioid treatment in the Tunisian population. METHODS: This prospective association study investigated seven variations in the OPRM1, OPRK1 and COMT gene, which encode Mu and KAPPA opioid receptors, and Catechol-O-methyltransferase enzyme respectively, in a cohort of 129 Tunisian cancer pain patients under oral morphine treatment. Genotyping was performed by simple probe probes on Light Cyler for rs17174629, rs1799972, rs1799971, rs1051659, rs1051660 and rs4680 and by PCR assay for the indel in the promoter region of OPRK1 (rs35566036). A statistical associations study between dose (continuous), dose escalation (yes/no) and SNP or haplotypes were investigated using linear multiple regressions and logistic regressions respectively adjusted on metastases and pain covariates in the R software. RESULTS: We detected significant association of the rs1051660 adjusted on metastasis and pain (P=0.02), no other association has been detected between the 7 polymorphisms screened and the dose of morphine needed for pain relief. CONCLUSION: This can be explained by the strong genetic heterogeneity in the cosmopolitan areas where our patients were recruited for this study, compared to more homegenous population recruited in other studies.
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spelling pubmed-57349712017-12-19 Genetic Analysis of Mu and Kappa Opioid Receptor and COMT Enzyme in Cancer Pain Tunisian Patients Under Opioid Treatment CHATTI, Imen WOILLARD, Jean-Baptiste MILI, Amira CREVEAUX, Isabelle BEN CHARFEDDINE, Ilhem FEKI, Jihène LANGLAIS, Sarah BEN FATMA, Leila SAAD, Ali GRIBAA, Moez LIBERT, Frédéric Iran J Public Health Original Article BACKGROUND: Pain and its opioid treatments are complex measurable traits. Responses to morphine in terms of pain control is likely to be determined by many factors, including the underlying pain sensitivity of the patient, along with nature and extent of the painful process, concomitant medications, genetic and other clinical and environmental factors. This study investigated genetic polymorphisms implicated in the inter-individual pain response variability to opioid treatment in the Tunisian population. METHODS: This prospective association study investigated seven variations in the OPRM1, OPRK1 and COMT gene, which encode Mu and KAPPA opioid receptors, and Catechol-O-methyltransferase enzyme respectively, in a cohort of 129 Tunisian cancer pain patients under oral morphine treatment. Genotyping was performed by simple probe probes on Light Cyler for rs17174629, rs1799972, rs1799971, rs1051659, rs1051660 and rs4680 and by PCR assay for the indel in the promoter region of OPRK1 (rs35566036). A statistical associations study between dose (continuous), dose escalation (yes/no) and SNP or haplotypes were investigated using linear multiple regressions and logistic regressions respectively adjusted on metastases and pain covariates in the R software. RESULTS: We detected significant association of the rs1051660 adjusted on metastasis and pain (P=0.02), no other association has been detected between the 7 polymorphisms screened and the dose of morphine needed for pain relief. CONCLUSION: This can be explained by the strong genetic heterogeneity in the cosmopolitan areas where our patients were recruited for this study, compared to more homegenous population recruited in other studies. Tehran University of Medical Sciences 2017-12 /pmc/articles/PMC5734971/ /pubmed/29259946 Text en Copyright© Iranian Public Health Association & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
CHATTI, Imen
WOILLARD, Jean-Baptiste
MILI, Amira
CREVEAUX, Isabelle
BEN CHARFEDDINE, Ilhem
FEKI, Jihène
LANGLAIS, Sarah
BEN FATMA, Leila
SAAD, Ali
GRIBAA, Moez
LIBERT, Frédéric
Genetic Analysis of Mu and Kappa Opioid Receptor and COMT Enzyme in Cancer Pain Tunisian Patients Under Opioid Treatment
title Genetic Analysis of Mu and Kappa Opioid Receptor and COMT Enzyme in Cancer Pain Tunisian Patients Under Opioid Treatment
title_full Genetic Analysis of Mu and Kappa Opioid Receptor and COMT Enzyme in Cancer Pain Tunisian Patients Under Opioid Treatment
title_fullStr Genetic Analysis of Mu and Kappa Opioid Receptor and COMT Enzyme in Cancer Pain Tunisian Patients Under Opioid Treatment
title_full_unstemmed Genetic Analysis of Mu and Kappa Opioid Receptor and COMT Enzyme in Cancer Pain Tunisian Patients Under Opioid Treatment
title_short Genetic Analysis of Mu and Kappa Opioid Receptor and COMT Enzyme in Cancer Pain Tunisian Patients Under Opioid Treatment
title_sort genetic analysis of mu and kappa opioid receptor and comt enzyme in cancer pain tunisian patients under opioid treatment
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5734971/
https://www.ncbi.nlm.nih.gov/pubmed/29259946
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