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Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody
Non-human primates (NHP) are suitable models for studying different aspects of the human system, including pathogenesis and protective immunity to many diseases. However, the lack of specific immunological reagents for neo-tropical monkeys, such as Saimiri sciureus, is still a major factor limiting...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735064/ https://www.ncbi.nlm.nih.gov/pubmed/29312325 http://dx.doi.org/10.3389/fimmu.2017.01814 |
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author | de Souza, Hugo Amorim dos Santos Costa-Correa, Edmar Henrique Bianco-Junior, Cesare Andrade, Márcia Cristina Ribeiro Lima-Junior, Josué da Costa Pratt-Riccio, Lilian Rose Daniel-Ribeiro, Cláudio Tadeu Totino, Paulo Renato Rivas |
author_facet | de Souza, Hugo Amorim dos Santos Costa-Correa, Edmar Henrique Bianco-Junior, Cesare Andrade, Márcia Cristina Ribeiro Lima-Junior, Josué da Costa Pratt-Riccio, Lilian Rose Daniel-Ribeiro, Cláudio Tadeu Totino, Paulo Renato Rivas |
author_sort | de Souza, Hugo Amorim dos Santos |
collection | PubMed |
description | Non-human primates (NHP) are suitable models for studying different aspects of the human system, including pathogenesis and protective immunity to many diseases. However, the lack of specific immunological reagents for neo-tropical monkeys, such as Saimiri sciureus, is still a major factor limiting studies in these models. An alternative strategy to circumvent this obstacle has been the selection of immunological reagents directed to humans, which present cross-reactivity with NHP molecules. In this context and considering the key role of inhibitory immunoreceptors—such as the signal regulatory protein α (SIRPα)—in the regulation of immune responses, in the present study, we attempted to evaluate the ability of anti-human SIRPα monoclonal antibodies to recognize SIRPα in antigen-presenting S. sciureus peripheral blood mononuclear cells (PBMC). As shown by flow cytometry analysis, the profile of anti-SIRPα staining as well as the levels of SIRPα-positive cells in PBMC from S. sciureus were similar to those observed in human PBMC. Furthermore, using anti-SIRPα monoclonal antibody, it was possible to detect a decrease of the SIRPα levels on surface of S. sciureus cells after in vitro stimulation with lipopolysaccharides. Finally, using computed-based analysis, we observed a high degree of conservation of SIRPα across six species of primates and the presence of shared epitopes in the extracellular domain between humans and Saimiri genus that could be targeted by antibodies. In conclusion, we have identified a commercially available anti-human monoclonal antibody that is able to detect SIRPα of S. sciureus monkeys and that, therefore, can facilitate the study of the immunomodulatory role of SIRPα when S. sciureus is used as a model. |
format | Online Article Text |
id | pubmed-5735064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57350642018-01-08 Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody de Souza, Hugo Amorim dos Santos Costa-Correa, Edmar Henrique Bianco-Junior, Cesare Andrade, Márcia Cristina Ribeiro Lima-Junior, Josué da Costa Pratt-Riccio, Lilian Rose Daniel-Ribeiro, Cláudio Tadeu Totino, Paulo Renato Rivas Front Immunol Immunology Non-human primates (NHP) are suitable models for studying different aspects of the human system, including pathogenesis and protective immunity to many diseases. However, the lack of specific immunological reagents for neo-tropical monkeys, such as Saimiri sciureus, is still a major factor limiting studies in these models. An alternative strategy to circumvent this obstacle has been the selection of immunological reagents directed to humans, which present cross-reactivity with NHP molecules. In this context and considering the key role of inhibitory immunoreceptors—such as the signal regulatory protein α (SIRPα)—in the regulation of immune responses, in the present study, we attempted to evaluate the ability of anti-human SIRPα monoclonal antibodies to recognize SIRPα in antigen-presenting S. sciureus peripheral blood mononuclear cells (PBMC). As shown by flow cytometry analysis, the profile of anti-SIRPα staining as well as the levels of SIRPα-positive cells in PBMC from S. sciureus were similar to those observed in human PBMC. Furthermore, using anti-SIRPα monoclonal antibody, it was possible to detect a decrease of the SIRPα levels on surface of S. sciureus cells after in vitro stimulation with lipopolysaccharides. Finally, using computed-based analysis, we observed a high degree of conservation of SIRPα across six species of primates and the presence of shared epitopes in the extracellular domain between humans and Saimiri genus that could be targeted by antibodies. In conclusion, we have identified a commercially available anti-human monoclonal antibody that is able to detect SIRPα of S. sciureus monkeys and that, therefore, can facilitate the study of the immunomodulatory role of SIRPα when S. sciureus is used as a model. Frontiers Media S.A. 2017-12-14 /pmc/articles/PMC5735064/ /pubmed/29312325 http://dx.doi.org/10.3389/fimmu.2017.01814 Text en Copyright © 2017 Souza, Costa-Correa, Bianco-Junior, Andrade, Lima-Junior, Pratt-Riccio, Daniel-Ribeiro and Totino. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology de Souza, Hugo Amorim dos Santos Costa-Correa, Edmar Henrique Bianco-Junior, Cesare Andrade, Márcia Cristina Ribeiro Lima-Junior, Josué da Costa Pratt-Riccio, Lilian Rose Daniel-Ribeiro, Cláudio Tadeu Totino, Paulo Renato Rivas Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
title | Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
title_full | Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
title_fullStr | Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
title_full_unstemmed | Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
title_short | Detection of Signal Regulatory Protein α in Saimiri sciureus (Squirrel Monkey) by Anti-Human Monoclonal Antibody |
title_sort | detection of signal regulatory protein α in saimiri sciureus (squirrel monkey) by anti-human monoclonal antibody |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735064/ https://www.ncbi.nlm.nih.gov/pubmed/29312325 http://dx.doi.org/10.3389/fimmu.2017.01814 |
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