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Efficient regeneration system for rapid multiplication of clean planting material of Ensete ventricosum (Welw.) Cheesman

Enset (Ensete ventricosum (Welw.) Cheesman) is an economically important staple food crop in Ethiopia, especially in the southern and southwestern regions. It is called “false banana” due to its resemblance to banana, but inability to produce any edible fruit. The crop is clonally propagated using f...

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Autores principales: Tripathi, Jaindra, Matheka, Jonathan, Merga, Ibsa, Gebre, Endale, Tripathi, Leena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735214/
https://www.ncbi.nlm.nih.gov/pubmed/29284987
http://dx.doi.org/10.1007/s11627-017-9867-9
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author Tripathi, Jaindra
Matheka, Jonathan
Merga, Ibsa
Gebre, Endale
Tripathi, Leena
author_facet Tripathi, Jaindra
Matheka, Jonathan
Merga, Ibsa
Gebre, Endale
Tripathi, Leena
author_sort Tripathi, Jaindra
collection PubMed
description Enset (Ensete ventricosum (Welw.) Cheesman) is an economically important staple food crop in Ethiopia, especially in the southern and southwestern regions. It is called “false banana” due to its resemblance to banana, but inability to produce any edible fruit. The crop is clonally propagated using field-grown suckers. This study reports the development of a robust regeneration technique to propagate large numbers of plantlets using corm discs containing intercalary meristematic tissues. Hundreds of shoot buds were induced from corm discs of enset cultivar ‘Bedadeti’ cultured on Murashige and Skoog (MS) medium supplemented with 1.5 mg L(−1) 2,4-dichlorophenoxyacetic acid, 0.216 mg L(−1) zeatin, and 2 g L(−1) activated charcoal. The shoot buds were regenerated into complete plantlets when transferred onto MS medium supplemented with 1 mg L(−1) 6-benzylaminopurine and 2 g L(−1) activated charcoal. More than 100 plantlets were generated in 4 mo from corm discs isolated from a single in vitro mother plantlet. Well-rooted plantlets were acclimatized in soil with 100% success, and did not show any apparent phenotypic abnormalities under glasshouse conditions. This efficient regeneration system could be very useful for the rapid multiplication of clean pathogen-free planting material.
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spelling pubmed-57352142017-12-26 Efficient regeneration system for rapid multiplication of clean planting material of Ensete ventricosum (Welw.) Cheesman Tripathi, Jaindra Matheka, Jonathan Merga, Ibsa Gebre, Endale Tripathi, Leena In Vitro Cell Dev Biol Plant Plant Tissue Culture Enset (Ensete ventricosum (Welw.) Cheesman) is an economically important staple food crop in Ethiopia, especially in the southern and southwestern regions. It is called “false banana” due to its resemblance to banana, but inability to produce any edible fruit. The crop is clonally propagated using field-grown suckers. This study reports the development of a robust regeneration technique to propagate large numbers of plantlets using corm discs containing intercalary meristematic tissues. Hundreds of shoot buds were induced from corm discs of enset cultivar ‘Bedadeti’ cultured on Murashige and Skoog (MS) medium supplemented with 1.5 mg L(−1) 2,4-dichlorophenoxyacetic acid, 0.216 mg L(−1) zeatin, and 2 g L(−1) activated charcoal. The shoot buds were regenerated into complete plantlets when transferred onto MS medium supplemented with 1 mg L(−1) 6-benzylaminopurine and 2 g L(−1) activated charcoal. More than 100 plantlets were generated in 4 mo from corm discs isolated from a single in vitro mother plantlet. Well-rooted plantlets were acclimatized in soil with 100% success, and did not show any apparent phenotypic abnormalities under glasshouse conditions. This efficient regeneration system could be very useful for the rapid multiplication of clean pathogen-free planting material. Springer US 2017-12-01 2017 /pmc/articles/PMC5735214/ /pubmed/29284987 http://dx.doi.org/10.1007/s11627-017-9867-9 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Plant Tissue Culture
Tripathi, Jaindra
Matheka, Jonathan
Merga, Ibsa
Gebre, Endale
Tripathi, Leena
Efficient regeneration system for rapid multiplication of clean planting material of Ensete ventricosum (Welw.) Cheesman
title Efficient regeneration system for rapid multiplication of clean planting material of Ensete ventricosum (Welw.) Cheesman
title_full Efficient regeneration system for rapid multiplication of clean planting material of Ensete ventricosum (Welw.) Cheesman
title_fullStr Efficient regeneration system for rapid multiplication of clean planting material of Ensete ventricosum (Welw.) Cheesman
title_full_unstemmed Efficient regeneration system for rapid multiplication of clean planting material of Ensete ventricosum (Welw.) Cheesman
title_short Efficient regeneration system for rapid multiplication of clean planting material of Ensete ventricosum (Welw.) Cheesman
title_sort efficient regeneration system for rapid multiplication of clean planting material of ensete ventricosum (welw.) cheesman
topic Plant Tissue Culture
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735214/
https://www.ncbi.nlm.nih.gov/pubmed/29284987
http://dx.doi.org/10.1007/s11627-017-9867-9
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