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Short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages

Diabetic status is characterized by chronic low-grade inflammation and an increased burden of senescent cells. Recently, the senescence-associated secretory phenotype (SASP) has been suggested as a possible source of inflammatory factors in obesity-induced type 2 diabetes. However, while senescence...

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Autores principales: Prattichizzo, Francesco, De Nigris, Valeria, Mancuso, Elettra, Spiga, Rosangela, Giuliani, Angelica, Matacchione, Giulia, Lazzarini, Raffaella, Marcheselli, Fiorella, Recchioni, Rina, Testa, Roberto, La Sala, Lucia, Rippo, Maria Rita, Procopio, Antonio Domenico, Olivieri, Fabiola, Ceriello, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735298/
https://www.ncbi.nlm.nih.gov/pubmed/29253812
http://dx.doi.org/10.1016/j.redox.2017.12.001
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author Prattichizzo, Francesco
De Nigris, Valeria
Mancuso, Elettra
Spiga, Rosangela
Giuliani, Angelica
Matacchione, Giulia
Lazzarini, Raffaella
Marcheselli, Fiorella
Recchioni, Rina
Testa, Roberto
La Sala, Lucia
Rippo, Maria Rita
Procopio, Antonio Domenico
Olivieri, Fabiola
Ceriello, Antonio
author_facet Prattichizzo, Francesco
De Nigris, Valeria
Mancuso, Elettra
Spiga, Rosangela
Giuliani, Angelica
Matacchione, Giulia
Lazzarini, Raffaella
Marcheselli, Fiorella
Recchioni, Rina
Testa, Roberto
La Sala, Lucia
Rippo, Maria Rita
Procopio, Antonio Domenico
Olivieri, Fabiola
Ceriello, Antonio
author_sort Prattichizzo, Francesco
collection PubMed
description Diabetic status is characterized by chronic low-grade inflammation and an increased burden of senescent cells. Recently, the senescence-associated secretory phenotype (SASP) has been suggested as a possible source of inflammatory factors in obesity-induced type 2 diabetes. However, while senescence is a known consequence of hyperglycaemia, evidences of SASP as a result of the glycaemic insult are missing. In addition, few data are available regarding which cell types are the main SASP-spreading cells in vivo. Adopting a four-pronged approach we demonstrated that: i) an archetypal SASP response that was at least partly attributable to endothelial cells and macrophages is induced in mouse kidney after in vivo exposure to sustained hyperglycaemia; ii) reproducing a similar condition in vitro in endothelial cells and macrophages, hyperglycaemic stimulus largely phenocopies the SASP acquired during replicative senescence; iii) in endothelial cells, hyperglycaemia-induced senescence and SASP could be prevented by SOD-1 overexpression; and iiii) ex vivo circulating angiogenic cells derived from peripheral blood mononuclear cells from diabetic patients displayed features consistent with the SASP. Overall, the present findings document a direct link between hyperglycaemia and the SASP in endothelial cells and macrophages, making the SASP a highly likely contributor to the fuelling of low-grade inflammation in diabetes.
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spelling pubmed-57352982017-12-21 Short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages Prattichizzo, Francesco De Nigris, Valeria Mancuso, Elettra Spiga, Rosangela Giuliani, Angelica Matacchione, Giulia Lazzarini, Raffaella Marcheselli, Fiorella Recchioni, Rina Testa, Roberto La Sala, Lucia Rippo, Maria Rita Procopio, Antonio Domenico Olivieri, Fabiola Ceriello, Antonio Redox Biol Research Paper Diabetic status is characterized by chronic low-grade inflammation and an increased burden of senescent cells. Recently, the senescence-associated secretory phenotype (SASP) has been suggested as a possible source of inflammatory factors in obesity-induced type 2 diabetes. However, while senescence is a known consequence of hyperglycaemia, evidences of SASP as a result of the glycaemic insult are missing. In addition, few data are available regarding which cell types are the main SASP-spreading cells in vivo. Adopting a four-pronged approach we demonstrated that: i) an archetypal SASP response that was at least partly attributable to endothelial cells and macrophages is induced in mouse kidney after in vivo exposure to sustained hyperglycaemia; ii) reproducing a similar condition in vitro in endothelial cells and macrophages, hyperglycaemic stimulus largely phenocopies the SASP acquired during replicative senescence; iii) in endothelial cells, hyperglycaemia-induced senescence and SASP could be prevented by SOD-1 overexpression; and iiii) ex vivo circulating angiogenic cells derived from peripheral blood mononuclear cells from diabetic patients displayed features consistent with the SASP. Overall, the present findings document a direct link between hyperglycaemia and the SASP in endothelial cells and macrophages, making the SASP a highly likely contributor to the fuelling of low-grade inflammation in diabetes. Elsevier 2017-12-06 /pmc/articles/PMC5735298/ /pubmed/29253812 http://dx.doi.org/10.1016/j.redox.2017.12.001 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Prattichizzo, Francesco
De Nigris, Valeria
Mancuso, Elettra
Spiga, Rosangela
Giuliani, Angelica
Matacchione, Giulia
Lazzarini, Raffaella
Marcheselli, Fiorella
Recchioni, Rina
Testa, Roberto
La Sala, Lucia
Rippo, Maria Rita
Procopio, Antonio Domenico
Olivieri, Fabiola
Ceriello, Antonio
Short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages
title Short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages
title_full Short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages
title_fullStr Short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages
title_full_unstemmed Short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages
title_short Short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages
title_sort short-term sustained hyperglycaemia fosters an archetypal senescence-associated secretory phenotype in endothelial cells and macrophages
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735298/
https://www.ncbi.nlm.nih.gov/pubmed/29253812
http://dx.doi.org/10.1016/j.redox.2017.12.001
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