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Isolation of equine peripheral blood stem cells from a Japanese native horse
The sizes of Japanese native horses have drastically decreased, and protection of these populations is important for Japanese horse culture. Social trials as well as scientific attempts are necessary for maintaining the breed. Mesenchymal stem cells (MSCs) have potential as a cell source for various...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Equine Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735313/ https://www.ncbi.nlm.nih.gov/pubmed/29270073 http://dx.doi.org/10.1294/jes.28.153 |
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author | ISHIKAWA, Shingo HORINOUCHI, Chie MIZOGUCHI, Ryugo SENOKUCHI, Asuka KAMIKAKIMOTO, Rie MURATA, Daiki HATAZOE, Takashi TOZAKI, Teruaki MISUMI, Kazuhiro HOBO, Seiji |
author_facet | ISHIKAWA, Shingo HORINOUCHI, Chie MIZOGUCHI, Ryugo SENOKUCHI, Asuka KAMIKAKIMOTO, Rie MURATA, Daiki HATAZOE, Takashi TOZAKI, Teruaki MISUMI, Kazuhiro HOBO, Seiji |
author_sort | ISHIKAWA, Shingo |
collection | PubMed |
description | The sizes of Japanese native horses have drastically decreased, and protection of these populations is important for Japanese horse culture. Social trials as well as scientific attempts are necessary for maintaining the breed. Mesenchymal stem cells (MSCs) have potential as a cell source for various cell therapies. However, there have been no reports on MSCs of Japanese native horses. We aimed to isolate and characterize MSCs from a Japanese native horse, the Noma horse. Plastic-adherent and self-replicating cells were isolated from a Noma horse’s peripheral blood (PB). The isolated cells had trilineage potential and a surface antigen of mesenchymal cells, so they fulfilled the minimal criteria of MSCs. Therefore, PB can be one source of MSCs for Japanese native horses. |
format | Online Article Text |
id | pubmed-5735313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Japanese Society of Equine Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57353132017-12-21 Isolation of equine peripheral blood stem cells from a Japanese native horse ISHIKAWA, Shingo HORINOUCHI, Chie MIZOGUCHI, Ryugo SENOKUCHI, Asuka KAMIKAKIMOTO, Rie MURATA, Daiki HATAZOE, Takashi TOZAKI, Teruaki MISUMI, Kazuhiro HOBO, Seiji J Equine Sci Note The sizes of Japanese native horses have drastically decreased, and protection of these populations is important for Japanese horse culture. Social trials as well as scientific attempts are necessary for maintaining the breed. Mesenchymal stem cells (MSCs) have potential as a cell source for various cell therapies. However, there have been no reports on MSCs of Japanese native horses. We aimed to isolate and characterize MSCs from a Japanese native horse, the Noma horse. Plastic-adherent and self-replicating cells were isolated from a Noma horse’s peripheral blood (PB). The isolated cells had trilineage potential and a surface antigen of mesenchymal cells, so they fulfilled the minimal criteria of MSCs. Therefore, PB can be one source of MSCs for Japanese native horses. The Japanese Society of Equine Science 2017-12-16 2017 /pmc/articles/PMC5735313/ /pubmed/29270073 http://dx.doi.org/10.1294/jes.28.153 Text en ©2017 The Japanese Society of Equine Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Note ISHIKAWA, Shingo HORINOUCHI, Chie MIZOGUCHI, Ryugo SENOKUCHI, Asuka KAMIKAKIMOTO, Rie MURATA, Daiki HATAZOE, Takashi TOZAKI, Teruaki MISUMI, Kazuhiro HOBO, Seiji Isolation of equine peripheral blood stem cells from a Japanese native horse |
title | Isolation of equine peripheral blood stem cells from a Japanese native horse |
title_full | Isolation of equine peripheral blood stem cells from a Japanese native horse |
title_fullStr | Isolation of equine peripheral blood stem cells from a Japanese native horse |
title_full_unstemmed | Isolation of equine peripheral blood stem cells from a Japanese native horse |
title_short | Isolation of equine peripheral blood stem cells from a Japanese native horse |
title_sort | isolation of equine peripheral blood stem cells from a japanese native horse |
topic | Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735313/ https://www.ncbi.nlm.nih.gov/pubmed/29270073 http://dx.doi.org/10.1294/jes.28.153 |
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