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The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells

AIM: The purpose of the current study was to investigate the effects of nephronectin (Npnt) in human dental pulp stem cells (hDPSCs). METHODOLOGY: Npnt was coated to nontissue culture-treated polystyrene (non-PS) plates. The presence of immobilized protein on the surface was detected by polyclonal r...

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Autores principales: Tang, Jia, Saito, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735320/
https://www.ncbi.nlm.nih.gov/pubmed/29358954
http://dx.doi.org/10.1155/2017/2546261
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author Tang, Jia
Saito, Takashi
author_facet Tang, Jia
Saito, Takashi
author_sort Tang, Jia
collection PubMed
description AIM: The purpose of the current study was to investigate the effects of nephronectin (Npnt) in human dental pulp stem cells (hDPSCs). METHODOLOGY: Npnt was coated to nontissue culture-treated polystyrene (non-PS) plates. The presence of immobilized protein on the surface was detected by polyclonal rabbit primary anti-Npnt antibody. Then the cell number was counted and compared with PBS-, bovine serum albumin- (BSA-), fish scale type I collagen- (FCOL1-), and human fibronectin- (Fn-) coated wells. Cell proliferation was assessed using CCK-8 assay. Cell morphology was observed under light microscopy and fluorescence microscopy. Lastly, the mRNA expression profiles of integrins, dentin sialophosphoprotein (DSPP), bone sialoprotein (BSP), and mineralization capacity of hDPSCs were investigated by real time RT-PCR and alizarin red staining, respectively. RESULTS: Npnt mediates hDPSC adhesion and spreading partially via the Arg-Gly-Asp (RGD) motif. Npnt enhanced the mRNA expression of ITGA1, ITGA4, ITGA7, and ITGB1 on day five. Npnt downregulated DSPP but significantly upregulated BSP mRNA expression at day 28. Further, Npnt and FCOL1 accelerated the matrix mineralization in hDPSCs. CONCLUSIONS: The current findings implicate that Npnt would be favorable to recruit hDPSCs and conducive to mineralization in hDPSCs. The combination of Npnt with hDPSCs may offer a promising approach for hard tissue regeneration.
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spelling pubmed-57353202018-01-22 The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells Tang, Jia Saito, Takashi Stem Cells Int Research Article AIM: The purpose of the current study was to investigate the effects of nephronectin (Npnt) in human dental pulp stem cells (hDPSCs). METHODOLOGY: Npnt was coated to nontissue culture-treated polystyrene (non-PS) plates. The presence of immobilized protein on the surface was detected by polyclonal rabbit primary anti-Npnt antibody. Then the cell number was counted and compared with PBS-, bovine serum albumin- (BSA-), fish scale type I collagen- (FCOL1-), and human fibronectin- (Fn-) coated wells. Cell proliferation was assessed using CCK-8 assay. Cell morphology was observed under light microscopy and fluorescence microscopy. Lastly, the mRNA expression profiles of integrins, dentin sialophosphoprotein (DSPP), bone sialoprotein (BSP), and mineralization capacity of hDPSCs were investigated by real time RT-PCR and alizarin red staining, respectively. RESULTS: Npnt mediates hDPSC adhesion and spreading partially via the Arg-Gly-Asp (RGD) motif. Npnt enhanced the mRNA expression of ITGA1, ITGA4, ITGA7, and ITGB1 on day five. Npnt downregulated DSPP but significantly upregulated BSP mRNA expression at day 28. Further, Npnt and FCOL1 accelerated the matrix mineralization in hDPSCs. CONCLUSIONS: The current findings implicate that Npnt would be favorable to recruit hDPSCs and conducive to mineralization in hDPSCs. The combination of Npnt with hDPSCs may offer a promising approach for hard tissue regeneration. Hindawi 2017 2017-11-19 /pmc/articles/PMC5735320/ /pubmed/29358954 http://dx.doi.org/10.1155/2017/2546261 Text en Copyright © 2017 Jia Tang and Takashi Saito. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tang, Jia
Saito, Takashi
The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells
title The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells
title_full The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells
title_fullStr The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells
title_full_unstemmed The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells
title_short The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells
title_sort role of nephronectin on proliferation and differentiation in human dental pulp stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735320/
https://www.ncbi.nlm.nih.gov/pubmed/29358954
http://dx.doi.org/10.1155/2017/2546261
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