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Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells

The aim of this study was to investigate the chemical constituents of Lindera erythrocarpa essential oil (LEO) by gas chromatography-mass spectrometry and evaluate their inhibitory effect on the expression of pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Fifteen c...

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Autores principales: Ko, Yeong-Jong, Ahn, Ginnae, Ham, Young-Min, Song, Sang-Mock, Ko, Eun-Yi, Cho, Su-Hyeon, Yoon, Weon-Jong, Kim, Kil-Nam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Leibniz Research Centre for Working Environment and Human Factors 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735349/
https://www.ncbi.nlm.nih.gov/pubmed/29285007
http://dx.doi.org/10.17179/excli2017-596
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author Ko, Yeong-Jong
Ahn, Ginnae
Ham, Young-Min
Song, Sang-Mock
Ko, Eun-Yi
Cho, Su-Hyeon
Yoon, Weon-Jong
Kim, Kil-Nam
author_facet Ko, Yeong-Jong
Ahn, Ginnae
Ham, Young-Min
Song, Sang-Mock
Ko, Eun-Yi
Cho, Su-Hyeon
Yoon, Weon-Jong
Kim, Kil-Nam
author_sort Ko, Yeong-Jong
collection PubMed
description The aim of this study was to investigate the chemical constituents of Lindera erythrocarpa essential oil (LEO) by gas chromatography-mass spectrometry and evaluate their inhibitory effect on the expression of pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Fifteen compounds, accounting for 63.7 % of the composition of LEO, were identified. The main compounds were nerolidol (18.73 %), caryophyllene (14.41 %), α-humulene (7.73 %), germacrene-D (4.82 %), and α-pinene (4.47 %). LEO significantly inhibited the expression of inducible nitric oxide (NO) synthase and cyclooxygenase-2, and subsequent production of NO and prostaglandin E(2). In addition, it reduced the release of pro-inflammatory cytokines in LPS-activated RAW264.7 cells. The molecular mechanism underlying the effect of LEO was associated with inhibition of the phosphorylation of mitogen-activated protein kinase (MAPK). Furthermore, LEO inhibited LPS-induced phosphorylation and degradation of inhibitor of kappa B-α, which is required for the activation of the p50 and p65 nuclear factor (NF)-κB subunits in RAW264.7 cells. Taken together, these data suggest that LEO exerted its anti-inflammatory effect by downregulating LPS-induced production of pro-inflammatory mediators through the inhibition of NF-κB and MAPK signaling in RAW264.7 cells.
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spelling pubmed-57353492017-12-28 Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells Ko, Yeong-Jong Ahn, Ginnae Ham, Young-Min Song, Sang-Mock Ko, Eun-Yi Cho, Su-Hyeon Yoon, Weon-Jong Kim, Kil-Nam EXCLI J Original Article The aim of this study was to investigate the chemical constituents of Lindera erythrocarpa essential oil (LEO) by gas chromatography-mass spectrometry and evaluate their inhibitory effect on the expression of pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Fifteen compounds, accounting for 63.7 % of the composition of LEO, were identified. The main compounds were nerolidol (18.73 %), caryophyllene (14.41 %), α-humulene (7.73 %), germacrene-D (4.82 %), and α-pinene (4.47 %). LEO significantly inhibited the expression of inducible nitric oxide (NO) synthase and cyclooxygenase-2, and subsequent production of NO and prostaglandin E(2). In addition, it reduced the release of pro-inflammatory cytokines in LPS-activated RAW264.7 cells. The molecular mechanism underlying the effect of LEO was associated with inhibition of the phosphorylation of mitogen-activated protein kinase (MAPK). Furthermore, LEO inhibited LPS-induced phosphorylation and degradation of inhibitor of kappa B-α, which is required for the activation of the p50 and p65 nuclear factor (NF)-κB subunits in RAW264.7 cells. Taken together, these data suggest that LEO exerted its anti-inflammatory effect by downregulating LPS-induced production of pro-inflammatory mediators through the inhibition of NF-κB and MAPK signaling in RAW264.7 cells. Leibniz Research Centre for Working Environment and Human Factors 2017-08-29 /pmc/articles/PMC5735349/ /pubmed/29285007 http://dx.doi.org/10.17179/excli2017-596 Text en Copyright © 2017 Ko et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited.
spellingShingle Original Article
Ko, Yeong-Jong
Ahn, Ginnae
Ham, Young-Min
Song, Sang-Mock
Ko, Eun-Yi
Cho, Su-Hyeon
Yoon, Weon-Jong
Kim, Kil-Nam
Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells
title Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells
title_full Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells
title_fullStr Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells
title_full_unstemmed Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells
title_short Anti-inflammatory effect and mechanism of action of Lindera erythrocarpa essential oil in lipopolysaccharide-stimulated RAW264.7 cells
title_sort anti-inflammatory effect and mechanism of action of lindera erythrocarpa essential oil in lipopolysaccharide-stimulated raw264.7 cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735349/
https://www.ncbi.nlm.nih.gov/pubmed/29285007
http://dx.doi.org/10.17179/excli2017-596
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