Cargando…

Assembly of an early-matured japonica (Geng) rice genome, Suijing18, based on PacBio and Illumina sequencing

The early-matured japonica (Geng) rice variety, Suijing18 (SJ18), carries multiple elite traits including durable blast resistance, good grain quality, and high yield. Using PacBio SMRT technology, we produced over 25 Gb of long-read sequencing raw data from SJ18 with a coverage of 62×. Using Illumi...

Descripción completa

Detalles Bibliográficos
Autores principales: Nie, Shou-Jun, Liu, Yu-Qiang, Wang, Chun-Chao, Gao, Shi-Wei, Xu, Tian-Tian, Liu, Qing, Chang, Hui-Lin, Chen, Yu-Bao, Yan, Peng-Cheng, Peng, Wei, Zheng, Tian-Qing, Xu, Jian-Long, Li, Zhi-Kang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735919/
https://www.ncbi.nlm.nih.gov/pubmed/29257136
http://dx.doi.org/10.1038/sdata.2017.195
Descripción
Sumario:The early-matured japonica (Geng) rice variety, Suijing18 (SJ18), carries multiple elite traits including durable blast resistance, good grain quality, and high yield. Using PacBio SMRT technology, we produced over 25 Gb of long-read sequencing raw data from SJ18 with a coverage of 62×. Using Illumina paired-end whole-genome shotgun sequencing technology, we generated 59 Gb of short-read sequencing data from SJ18 (23.6 Gb from a 200 bp library with a coverage of 59× and 35.4 Gb from an 800 bp library with a coverage of 88×). With these data, we assembled a single SJ18 genome and then generated a set of annotation data. These data sets can be used to test new programs for variation deep mining, and will provide new insights into the genome structure, function, and evolution of SJ18, and will provide essential support for biological research in general.