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Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease

European common ash, Fraxinus excelsior, is currently threatened by Ash dieback (ADB) caused by the fungus, Hymenoscyphus fraxineus. To detect and identify metabolites that may be products of pathways important in contributing to resistance against H. fraxineus, we performed untargeted metabolomic p...

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Autores principales: Sambles, Christine M., Salmon, Deborah L., Florance, Hannah, Howard, Thomas P., Smirnoff, Nicholas, Nielsen, Lene R., McKinney, Lea V., Kjær, Erik D., Buggs, Richard J. A., Studholme, David J., Grant, Murray
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735976/
https://www.ncbi.nlm.nih.gov/pubmed/29257137
http://dx.doi.org/10.1038/sdata.2017.190
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author Sambles, Christine M.
Salmon, Deborah L.
Florance, Hannah
Howard, Thomas P.
Smirnoff, Nicholas
Nielsen, Lene R.
McKinney, Lea V.
Kjær, Erik D.
Buggs, Richard J. A.
Studholme, David J.
Grant, Murray
author_facet Sambles, Christine M.
Salmon, Deborah L.
Florance, Hannah
Howard, Thomas P.
Smirnoff, Nicholas
Nielsen, Lene R.
McKinney, Lea V.
Kjær, Erik D.
Buggs, Richard J. A.
Studholme, David J.
Grant, Murray
author_sort Sambles, Christine M.
collection PubMed
description European common ash, Fraxinus excelsior, is currently threatened by Ash dieback (ADB) caused by the fungus, Hymenoscyphus fraxineus. To detect and identify metabolites that may be products of pathways important in contributing to resistance against H. fraxineus, we performed untargeted metabolomic profiling on leaves from five high-susceptibility and five low-susceptibility F. excelsior individuals identified during Danish field trials. We describe in this study, two datasets. The first is untargeted LC-MS metabolomics raw data from ash leaves with high-susceptibility and low-susceptibility to ADB in positive and negative mode. These data allow the application of peak picking, alignment, gap-filling and retention-time correlation analyses to be performed in alternative ways. The second, a processed dataset containing abundances of aligned features across all samples enables further mining of the data. Here we illustrate the utility of this dataset which has previously been used to identify putative iridoid glycosides, well known anti-herbivory terpenoid derivatives, and show differential abundance in tolerant and susceptible ash samples.
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spelling pubmed-57359762017-12-21 Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease Sambles, Christine M. Salmon, Deborah L. Florance, Hannah Howard, Thomas P. Smirnoff, Nicholas Nielsen, Lene R. McKinney, Lea V. Kjær, Erik D. Buggs, Richard J. A. Studholme, David J. Grant, Murray Sci Data Data Descriptor European common ash, Fraxinus excelsior, is currently threatened by Ash dieback (ADB) caused by the fungus, Hymenoscyphus fraxineus. To detect and identify metabolites that may be products of pathways important in contributing to resistance against H. fraxineus, we performed untargeted metabolomic profiling on leaves from five high-susceptibility and five low-susceptibility F. excelsior individuals identified during Danish field trials. We describe in this study, two datasets. The first is untargeted LC-MS metabolomics raw data from ash leaves with high-susceptibility and low-susceptibility to ADB in positive and negative mode. These data allow the application of peak picking, alignment, gap-filling and retention-time correlation analyses to be performed in alternative ways. The second, a processed dataset containing abundances of aligned features across all samples enables further mining of the data. Here we illustrate the utility of this dataset which has previously been used to identify putative iridoid glycosides, well known anti-herbivory terpenoid derivatives, and show differential abundance in tolerant and susceptible ash samples. Nature Publishing Group 2017-12-19 /pmc/articles/PMC5735976/ /pubmed/29257137 http://dx.doi.org/10.1038/sdata.2017.190 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.
spellingShingle Data Descriptor
Sambles, Christine M.
Salmon, Deborah L.
Florance, Hannah
Howard, Thomas P.
Smirnoff, Nicholas
Nielsen, Lene R.
McKinney, Lea V.
Kjær, Erik D.
Buggs, Richard J. A.
Studholme, David J.
Grant, Murray
Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease
title Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease
title_full Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease
title_fullStr Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease
title_full_unstemmed Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease
title_short Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease
title_sort ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735976/
https://www.ncbi.nlm.nih.gov/pubmed/29257137
http://dx.doi.org/10.1038/sdata.2017.190
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