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DNA methyltransferase 3A gene polymorphism contributes to daily life stress susceptibility
Daily life stress markedly affects the response toward stressful stimuli. DNA methy-lation is one of the factors that regulate this response, and is a normal mechanism of somatic cell growth, but its regulatory gene variations may cause alterations in the stress response. The aim of the present stud...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735991/ https://www.ncbi.nlm.nih.gov/pubmed/29290696 http://dx.doi.org/10.2147/PRBM.S152451 |
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author | Barliana, Melisa I Amalya, Shintya N Pradipta, Ivan S Alfian, Sofa D Kusuma, Arif SW Milanda, Tiana Abdulah, Rizky |
author_facet | Barliana, Melisa I Amalya, Shintya N Pradipta, Ivan S Alfian, Sofa D Kusuma, Arif SW Milanda, Tiana Abdulah, Rizky |
author_sort | Barliana, Melisa I |
collection | PubMed |
description | Daily life stress markedly affects the response toward stressful stimuli. DNA methy-lation is one of the factors that regulate this response, and is a normal mechanism of somatic cell growth, but its regulatory gene variations may cause alterations in the stress response. The aim of the present study was to investigate genotypic variants of the DNA methyltransferase 3A (DNMT3A) gene in 129 healthy subjects and evaluate its association with daily life stress. Blood samples were collected, and genomic DNA was isolated. DNA was amplified using specific tetra primers for DNMT3A (C/T) rs11683424 and visualized following 2% agarose gel electrophoresis. The association of DNMT3A genetic variants with daily life stress was analyzed using the Kessler Psychological Distress Scale (K10). We observed that the distribution of subjects with genotype CC (wild type), CT (heteromutant), and TT (homomutant) was 13.95%, 81.4%, and 4.65%, respectively. Genetic variations significantly affected the daily life stress condition (p=0.04) in Indonesian healthy subjects, but most of the subjects with the CT phenotype were classified in a stress condition. |
format | Online Article Text |
id | pubmed-5735991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-57359912017-12-29 DNA methyltransferase 3A gene polymorphism contributes to daily life stress susceptibility Barliana, Melisa I Amalya, Shintya N Pradipta, Ivan S Alfian, Sofa D Kusuma, Arif SW Milanda, Tiana Abdulah, Rizky Psychol Res Behav Manag Original Research Daily life stress markedly affects the response toward stressful stimuli. DNA methy-lation is one of the factors that regulate this response, and is a normal mechanism of somatic cell growth, but its regulatory gene variations may cause alterations in the stress response. The aim of the present study was to investigate genotypic variants of the DNA methyltransferase 3A (DNMT3A) gene in 129 healthy subjects and evaluate its association with daily life stress. Blood samples were collected, and genomic DNA was isolated. DNA was amplified using specific tetra primers for DNMT3A (C/T) rs11683424 and visualized following 2% agarose gel electrophoresis. The association of DNMT3A genetic variants with daily life stress was analyzed using the Kessler Psychological Distress Scale (K10). We observed that the distribution of subjects with genotype CC (wild type), CT (heteromutant), and TT (homomutant) was 13.95%, 81.4%, and 4.65%, respectively. Genetic variations significantly affected the daily life stress condition (p=0.04) in Indonesian healthy subjects, but most of the subjects with the CT phenotype were classified in a stress condition. Dove Medical Press 2017-12-15 /pmc/articles/PMC5735991/ /pubmed/29290696 http://dx.doi.org/10.2147/PRBM.S152451 Text en © 2017 Barliana et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Barliana, Melisa I Amalya, Shintya N Pradipta, Ivan S Alfian, Sofa D Kusuma, Arif SW Milanda, Tiana Abdulah, Rizky DNA methyltransferase 3A gene polymorphism contributes to daily life stress susceptibility |
title | DNA methyltransferase 3A gene polymorphism contributes to daily life stress susceptibility |
title_full | DNA methyltransferase 3A gene polymorphism contributes to daily life stress susceptibility |
title_fullStr | DNA methyltransferase 3A gene polymorphism contributes to daily life stress susceptibility |
title_full_unstemmed | DNA methyltransferase 3A gene polymorphism contributes to daily life stress susceptibility |
title_short | DNA methyltransferase 3A gene polymorphism contributes to daily life stress susceptibility |
title_sort | dna methyltransferase 3a gene polymorphism contributes to daily life stress susceptibility |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735991/ https://www.ncbi.nlm.nih.gov/pubmed/29290696 http://dx.doi.org/10.2147/PRBM.S152451 |
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